Method for simultaneously detecting trifloxystrobin and metabolite trifloxystrobin acid residual in rice by LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)

Abstract

The invention discloses a method for simultaneously detecting trifloxystrobin and metabolite trifloxystrobin acid residual in rice by LC-MS / MS (Liquid Chromatography-Tandem Mass Spectrometry). The method comprises the following steps: extracting a to-be-detected rice sample; salting out a sample extracting solution; adding sodium chloride in the sample extracting solution for salting out, oscillating and centrifuging; purifying the sample subjected to salting out and diluting with acetonitrile; simultaneously detecting the trifloxystrobin and the metabolite o trifloxystrobin acid in the sample analysis solution by using a UPLC-MS / MS (Ultra High Performance LC-MS / MS) method. According to the method disclosed by the invention, a rice test sample is extracted with water and an acetonitrile mixed solution containing formic acid, then is purified by a matrix solid-phase extraction technology, and is subsequently and simultaneously detected under a multi-reaction monitoring (MRM) mode by using the UPLC-MS / MS, so that the simultaneous detection efficiency is improved, and the simultaneous detection cost is reduced; the method has the advantages of low detection limit, high sensitivity, good repeatability and high addition standard recovery rate.

Description

Technical field [0001] The present invention relates to a method for simultaneously detecting trifloxystrobin and metabolite trifloxylic acid residues in rice by LC-MS / MS, and particularly relates to the simultaneous detection of oxime in rice using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). The method of mycostrobin and its metabolite trifuric acid belongs to the field of simultaneous detection of food safety. Background technique [0002] Trifloxystrobin broad-spectrum fungicides are a new class of fluorinated fungicides successfully developed from the natural product Strobilurins as lead fungicide compounds. It has the characteristics of high efficiency, broad spectrum, protection, treatment, eradication, penetration, systemic activity, resistance to rain erosion, and long lasting effect. It is effective against strains resistant to 1,4-demethylase inhibitors, benzamides, dicarboxylamines and benzimidazole, and has no cross-resis...

AI Technical Summary

Problems solved by technology

However, the matrix of rice is complex, especially when the purification of the sample matrix is ​​not complete, the target compound cannot be effectively separated on the chromatographic column, and the target compound cannot be accurately quantified, and there is currently no method that can simultaneously detect trifloxystrobin and metabolites in rice. Effective method of oxalic acid

Images