High-simulation tissue engineering nerve repairing scaffold and making method thereof

A technology for simulating tissue and nerve repair, applied in the field of nerve repair tissue engineering, can solve problems such as the inability to precisely control pore size, spatial distribution and spatial structure, achieve good biocompatibility and biological activity, mild reaction conditions, and process technology. mature effect

Inactive Publication Date: 2017-04-26
SHANGXIAN MINIMAL INVASIVE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that none of the previous preparation methods of scaffold materials can accurately control the size, spatial distribution and spatial structure of pores, and provide a highly simulated tissue engineering nerve repair scaffold and its preparation method

Method used

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  • High-simulation tissue engineering nerve repairing scaffold and making method thereof
  • High-simulation tissue engineering nerve repairing scaffold and making method thereof
  • High-simulation tissue engineering nerve repairing scaffold and making method thereof

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preparation example Construction

[0023] A preparation method of a highly simulated tissue engineering nerve repair scaffold provided by the present invention comprises:

[0024] Preparation of Type I Collagen-Chitosan Suspension: Weigh Type I Collagen and Chitosan respectively according to the mass ratio of 1-10:1. Dissolve type I collagen in 0-4 mg / ml acetic acid or malonic acid solution for 2 hours, then stir in a constant temperature environment at 4°C to make a suspension; add chitosan to 0-4 mg / ml acetic acid Or fully dissolved in malonic acid solution, mix the two suspensions and keep stirring at a constant temperature of 4°C, fully mix to make type Ι collagen-chitosan suspension, and vacuumize and stand for 12 hours.

[0025] Injection molding and cold shower treatment: inject type Ι collagen-chitosan suspension into the silicone tube and seal the two ends, then slowly immerse the molded sample in the deep-low temperature cold shower (liquid nitrogen), enter the speed control 1×10 -6 m / s~1×10 -4 m / s...

Embodiment 1

[0038] Embodiment 1 provides a kind of preparation method of high-simulation tissue engineering nerve repair support, carries out according to the following steps:

[0039] 1. Preparation of Type I Collagen-Chitosan Suspension: Weigh Type I Collagen and Chitosan in a mass ratio of 4:1. Dissolve type I collagen in 1.5mg / ml malonic acid solution for 2 hours, then stir in a constant temperature environment at 4°C to make a suspension; add chitosan to 1.5mg / ml malonic acid solution Fully dissolve, mix the two suspensions and keep stirring at a constant temperature of 4°C, fully mix to form a type Ι collagen-chitosan gel-like suspension, vacuumize and stand for 12 hours;

[0040] 2. Injection molding and improved cold leaching treatment: Inject the suspension into the silicone tube and seal both ends, then slowly immerse the molded sample in the deep low temperature cold leaching agent (liquid nitrogen), and the entry speed is controlled to 2×10 -5 m / s, carry out gradient cold sho...

Embodiment 2

[0045] Embodiment 2 provides a kind of preparation method of highly simulated tissue engineered nerve repair scaffold, according to the method of embodiment 1, difference is: according to type I collagen protein and chitosan mass ratio is 10:1, adopts malonic acid The solution concentration is 0.5mg / mL, and the gradient cold shower speed is controlled at 1×10 -6 m / s, the raw material composition of the protective film is 100% polycaprolactone, the loading voltage used for electrospinning is 5kV, the feed rate of electrospinning is 0.5mL / h, and the mass concentration of genipin solution used is 0.5%. Nepin cross-linking time is set to 12h. All the other are identical with embodiment 1.

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Abstract

The invention discloses a making method of a high-simulation tissue engineering nerve repairing scaffold. The method comprises the following steps: carrying out injection molding on an I-type collagen-chitosan suspension, carrying out cold sprinkling, carrying out freeze drying molding, coating the obtained molded product with a protection film, and crosslinking the coated product and genipin to obtain the high-simulation tissue engineering nerve repairing scaffold. The method has the advantages of no special devices in the making process, mild reaction conditions, manure technology, and suitableness for large-scale production of modified h high-simulation tissue engineering nerve repairing scaffolds, and the produced repairing scaffold is in favor of stitching notches in operations, and has good biocompatibility and bioactivity.

Description

technical field [0001] The invention relates to the technical field of nerve repair tissue engineering, in particular to a scaffold for tissue engineering nerve repair and a preparation method thereof. Background technique [0002] Peripheral nerve regeneration and functional restoration are unresolved clinical problems. For short-segment nerve injuries, the severed nerve can be directly anastomosed end-to-end, so that the proximal regenerated nerve fibers can grow into the distal end. However, for long-segment nerve defects, tension-free suturing cannot be performed clinically. The current gold standard of treatment is to transplant autologous nerves from undamaged areas to damaged areas to bridge the damaged nerve stumps. However, the application of autologous nerve transplantation is limited by many factors: second surgery is required to obtain the donor nerve, insufficient source of nerve in the donor area, secondary loss of nerve function in the donor area, and mismatc...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/26A61L27/50D04H1/728D04H1/4382C08J7/12
CPCA61L27/34A61L27/26A61L27/50A61L2300/412A61L2430/32C08J7/12C08J2367/04C08J2389/00C08J2405/08C08J2467/04C08J2489/00D04H1/4382D04H1/728C08L67/04C08L5/08
Inventor 程玮璐蔡盼盼李洪谊郭凯陈玉龙王耀涓李贵阳高健赵志平
Owner SHANGXIAN MINIMAL INVASIVE INC
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