Degradation strain of fungicide pyraclostrobin, microbial inoculum prepared from degradation strain and application of degradation strain
A technology of pyraclostrobin and degrading bacteria, which is applied in the field of microbial degradation of pollutants, can solve the problems of pyraclostrobin pesticide residues exceeding the standard and environmental pollution, and achieve the problem of excessive pesticide residues and environmental pollution. The effect of biodegradation effect
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Embodiment 1
[0041] Example 1 Isolation, screening and identification of degrading strain BA01
[0042] 1. Isolation and screening of strains
[0043] (1) Samples: collected from activated sludge in sewage treatment tanks in Singapore.
[0044] (2) The separation and screening method is:
[0045] Take 5g of activated sludge sample and add it to 50mL basal medium, and add pyraclostrobin technical mother liquor (DMSO as solvent) at the same time, so that the final mass concentration of pyraclostrobin is 25mg / L. The enrichment culture of degrading microorganisms was carried out under the shaking table. After culturing for 7 days, transfer them to the second batch of basal medium containing 50 mg / L pyraclostrobin at an inoculum size of 10%. After culturing under the same conditions for 7 days, transfer them to the basal medium containing 100 mg / L pyraclostrobin at an inoculum size of 10%, and continue culturing for 7 days. By analogy, the mass concentration of pyraclostrobin was continuous...
Embodiment 2
[0054] Example 2 Preparation of bacterial strain BA01 degrading bacterial agent
[0055] The production process of using the above-mentioned strain BA01 to prepare the degrading bacterial agent is as follows: slant seed — shake flask seed solution — seed tank cultivation — production tank fermentation — product (the packaging dosage form is liquid bacterial agent).
[0056] The specific method is as follows:
[0057] Activate the bacteria of the degrading strain BA01 on the LB solid plate and inoculate it on the inclined surface of the test tube for later use.
[0058] Inoculate the test tube seed of degrading strain BA01 into a 1000 mL shake flask containing 250 mL of LB medium (the formula of LB medium is: 0.5% yeast extract, 1% peptone, 1% NaCl, pH value 7.2-7.5), 30°C Shake at a constant temperature until it reaches the logarithmic phase, and prepare to inoculate the seed tank. The seed tank is filled with seed medium, and the filling volume is 70%. At 1.1 Kg / cm2 after f...
Embodiment 3
[0061] Example 3 Laboratory Degradation Experiment
[0062] 1. Add the fungicide pyraclostrobin to the basal medium (same as Example 1) to make the final concentration 50mg / L; the inoculation concentration is 1.0×10 7 CFU / mL of BA01 bacterial agent, and the culture medium without inoculation was used as the control, cultured at 30°C for 5 days, samples were taken regularly, the residual amount of pesticide was determined by HPLC method, and the degradation rate was calculated.
[0063] The calculation method of degradation rate is as follows:
[0064]
[0065] 2. The results are shown in Table 1.
[0066] Table 1 Degradation effect of pyraclostrobin by degrading strain BA01
[0067]
[0068] The results showed that the degrading strain BA01 could effectively degrade pyraclostrobin in a short period of time, the degradation rate of pyraclostrobin reached 85.7% within 3 days, and the degradation rate of pyraclostrobin reached 92.3% within 5 days. Better biodegradation ...
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