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Gel dressing based on G-quadruplex/collagen, as well as preparation and application of gel dressing

A collagen and quadruplet technology, applied in bandages, absorbent pads, medical science and other directions, can solve the problems of complicated preparation of gel dressings, poor biocompatibility, long healing time, etc., and achieve accelerated wound healing and strong adhesion. , the effect of non-toxic side effects

Active Publication Date: 2017-05-10
EAST CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies of the prior art, the object of the present invention is to solve the complicated problem of preparation of the gel dressing, overcome the deficiencies of the gel dressing such as poor air permeability, long healing time, and poor biocompatibility; select degradable, biocompatible Using G-quadruplex and collagen as raw materials, the prepared G-quadruplex / collagen-based gel dressing has good mechanical properties, keeps the wound moisturized, adheres and avoids infection, effectively, Promotes rapid wound healing

Method used

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  • Gel dressing based on G-quadruplex/collagen, as well as preparation and application of gel dressing
  • Gel dressing based on G-quadruplex/collagen, as well as preparation and application of gel dressing
  • Gel dressing based on G-quadruplex/collagen, as well as preparation and application of gel dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Add guanosine, boric acid and potassium hydroxide into deionized water at a molar ratio of 1:1:1, shake for 5 minutes, heat the water bath to 90°C, and keep the temperature for 20 minutes until the guanosine is completely dissolved .

[0048] (2) Slowly cool the above solution to 37°C, keep the water bath at a constant temperature, add collagen to make the concentration of collagen in the solution 5mg / mL, shake it to make it disperse quickly, cool to room temperature, and prepare the G-quadruple Body / collagen gel dressing.

Embodiment 2

[0050] (1) Add guanosine, boric acid and potassium hydroxide into deionized water at a molar ratio of 2:1:1, shake for 10 minutes, heat the water bath to 95°C, and keep the constant temperature for 15 minutes until the guanosine is completely dissolve.

[0051] (2) Slowly cool the above solution to 40°C, keep the water bath at a constant temperature, add collagen so that the concentration of collagen in the solution is 7 mg / mL, shake it to disperse it quickly, and cool it to room temperature to prepare the G-based - Tetrad / collagen gel dressing. Prepared gel see figure 2 shown. When the prepared gel is placed upside down, the gel will stick to the bottle wall, unlike the aqueous solution that will flow down; and after 15 days, the gel will still stick to the bottle wall when placed upside down. This illustrates the good mechanical properties of the G-tetrad / collagen based gel dressing.

Embodiment 3

[0053] The gel prepared in Experimental Example 2 was used for wound healing of mice as a treatment group.

[0054]In this experiment, a traumatic scar model was made on the back of ICR mice by surgical method. By applying the gel on the surface of the wound, observe the healing of the surface scars every day, and evaluate the repairing effect of the product on the scars.

[0055] (1) modeling

[0056] Under anesthesia by intraperitoneal injection of 1% pentobarbital sodium 5mL / kg, the back of the mouse was sheared, depilated with 4% sodium sulfide, and a circular incision with a diameter of about 1.5 cm was made on both sides of the parallel spine. If there is slight bleeding, wipe the mouse with a sterile cotton ball to stop the bleeding, and return it to the mouse box.

[0057] (2) Animal grouping

[0058] The successfully modeled ICR mice were randomly divided into groups a, b, and c, with 3 mice in each group.

[0059] A control group: the wound on the right side of t...

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Abstract

The invention discloses a gel dressing based on G-quadruplex / collagen and a preparation method of the gel dressing. The preparation method comprises the following steps: adding guanine nucleoside, boric acid and potassium hydroxide to deionized water according to the proportion of the guanine nucleoside, to the boric acid to the potassium hydroxide being (1-3):1:1 and performing heating to 90-98 DEG C to obtain a G-quadruplex solution; cooling the G-quadruplex solution to 35-40 DEG C, adding collagen to disperse the solution till the concentration of the collagen in the solution is 2-10mg / mL, and performing cooling to room temperature so as to obtain the gel dressing based on G-quadruplex / collagen. The preparation method has the advantages of simple reaction conditions, few synthesis steps, short time and low production cost. When being used for wound healing, the gel dressing has good biocompatibility, good air permeability, strong adhesion properties, and high water content, and maintains humid environment, so that the wound is not liable to scab. The gel dressing disclosed by the invention does not contain any irritating ingredients, and is free from toxic and side effects. The gel dressing is used for accelerating wound healing and has a potential application value.

Description

technical field [0001] The invention belongs to the field of medical dressings, and relates to a gel dressing and a preparation method thereof, in particular to a G-quadruplex / collagen-based gel dressing capable of promoting wound healing and a preparation method thereof. Background technique [0002] Wound is the damage of normal skin caused by external factors such as surgery, heat, external force, electric current, low temperature, etc. If left untreated, the wound may become infected or worsen. Traditionally used dressings are gauze, cotton and other materials, but the effects of these dressings on hemostasis, moisturizing, preventing wound infection and promoting wound healing are not satisfactory. [0003] Collagen is the main component of animal connective tissue and the most abundant functional protein in mammals. Collagen has been widely used as a biomaterial due to its low immunogenicity, degradability, biocompatibility, and coagulation properties. Compared with...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/20A61L15/42
CPCA61L15/20A61L15/325A61L15/42A61L15/425A61L2300/23A61L2300/252A61L2300/412C08L89/00
Inventor 裴昊肖明书李丽瞿祥猛
Owner EAST CHINA NORMAL UNIVERSITY
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