Preparation method of collagen-hydroxyapatite scaffold containing nano silicon nitride

Abstract

The invention relates to a preparation method of a collagen-hydroxyapatite scaffold containing nano silicon nitride. The preparation method includes the steps of: performing a liquid-phase reduction reaction to prepare the nano silicon nitride, uniformly mixing the nano silicon nitride with a collagen-hydroxyapatite solution, and performing one-step freeze-drying and crosslinking to produce the scaffold, wherein concentration range of the nano silicon nitride is 10-50 [mu]g / ml and particle size of the nano silicon nitride is 10-30 nm. The raw materials of the scaffold are simple. The method is free of complex technology and high-end equipment, is low in cost and is easy to promote. A reasonable nano silicon nitride slow release system is provided for a wound surface, so that long-acting effective slow releasing of a medicine is provided for the wound surface, thereby maintaining the medicine concentration of the wound surface to be balanced and effective without excess.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a collagen-hydroxyapatite scaffold containing nanometer silicon nitride. Background technique [0002] Hydroxyapatite and collagen are the most important inorganic and organic components in natural bone, and both have good biological properties, but they have different defects when used alone, and it is difficult to meet the performance requirements of tissue engineering for biomaterials. The combination of collagen and hydroxyapatite makes better use of the cohesiveness of collagen and overcomes the fluidity of hydroxyapatite particles. [0003] Nano-silicon nitride has excellent heat transfer, electrical conductivity, surface activity and catalytic properties, and has great application value in the fields of optics, catalysis, microelectronics, biosensing, and antibacterial. Nano-silicon nitride powders with different shapes have differ...

AI Technical Summary

Problems solved by technology

[0004] Due to the high biological activity of nano-silicon nitride, when it is applied to tissue engineering, it will be released to the wound surface or target organ, and it will be preferentially captured by related proteins, chloride ions and groups in the wound exudate or tissue, resulting in nano-nitrogen Silicon nitride particles are abnormally aggregated at high concentrations in certain parts. Although they can effectively participate in antibacterial activities, these excess nano-silicon nitride will accumulate in epidermal cells, macrophages, and fibroblasts, leading to cell apoptosis and eventually leading to wound damage. non-union or delayed healing

In general, unrestrained use or uncontrolled release of nano-silicon nitride is bound to be counterproductive and not beneficial to wound repair