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DNA (Deoxyribonucleic Acid) hybridized micro-fluidic detector based on optical fiber micro-cavity echo wall

A detector and whispering gallery technology, applied in the field of sensing and detection, can solve the problems of harsh ultra-clean experimental environment, achieve the effects of enhancing evanescent field strength, improving robustness, and reducing consumption

Active Publication Date: 2017-05-10
NANKAI UNIV
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Problems solved by technology

However, optical fibers with a diameter of less than 2 microns are very fragile, air dust pollution and disturbances have a great impact on thin tapered optical fibers, and the ultra-clean experimental environment is harsh

Method used

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  • DNA (Deoxyribonucleic Acid) hybridized micro-fluidic detector based on optical fiber micro-cavity echo wall
  • DNA (Deoxyribonucleic Acid) hybridized micro-fluidic detector based on optical fiber micro-cavity echo wall
  • DNA (Deoxyribonucleic Acid) hybridized micro-fluidic detector based on optical fiber micro-cavity echo wall

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Embodiment 1

[0021] Such as figure 1 As shown, the DNA hybridization microfluidic detector based on the fiber optic microcavity whispering gallery of the present invention includes: 5 single-mode optical fibers, 7 tapered micro-fibers and 8 hollow-core silica fibers, wherein two of the tapered micro-fibers 7 The two ends of the overall air "U" microcavity structure pass through the single-mode fiber 5 and the stable narrow-band laser respectively. A light source 1 is connected to a high-resolution spectrometer 2 . The hollow-core silica fiber 8 and the tapered micro-fiber 7 are vertically placed in the "U"-shaped air microcavity, and the two ends of the hollow-core silica fiber are respectively connected to the peristaltic syringe pump 3 and the waste liquid cup 4 through the microfluidic tube 6 .

[0022] Schematic diagram of DNA hybridization detection in the hollow-core optical fiber microfluidic channel, such as figure 2 As shown, the inner surface of the hollow-core silica fiber is...

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Abstract

The invention provides a DNA (Deoxyribonucleic Acid) hybridized micro-fluidic detector based on an optical fiber micro-cavity echo wall. The DNA hybridized micro-fluidic detector comprises a tapered micro-optical fiber assisted U-shaped micro-cavity and a hollow-core quartz optical fiber, wherein the hollow-core quartz optical fiber and a micro-optical fiber are vertically arranged in the U-shaped micro-cavity. Large evanescent field coupling can be provided and an echo wall mode in a quartz optical fiber wall can be stimulated, without the need of utilizing the very thin micro-optical fiber. A polylysine molecule and a single-chain DNA probe molecule are modified on the surface of the inner wall of the hollow-core quartz optical fiber by utilizing an ion adsorption effect; and the refractive index in the quartz optical fiber is changed by hybridization between a target ssDNA molecule and an ssDNA probe molecule, so that the effect of the DNA hybridized micro-fluidic detector is realized. The DNA hybridized micro-fluidic detector has the advantages of high robustness, high sensitivity, low detection limit, trace sample consumption, high integration, high degree of automation and the like, and has great meaning on establishment of a high-integration biochip laboratory.

Description

technical field [0001] The invention relates to an optical fiber whispering gallery DNA hybrid microfluidic detector, in particular to a microcavity whispering gallery DNA hybrid microfluidic detector based on micro-nano optical fiber assistance, and belongs to the technical field of sensing and detection. [0002] Compared with traditional DNA detection methods, its performance solves the problem of trace DNA sample detection in terms of detection sensitivity and miniaturized size. Background technique [0003] Since the implementation of the Human Genome Project, more and more biological gene sequences have been determined, but once a certain sequence is confused, the genetic detection technology of rapid sequencing and accurate identification of disordered sequences will become the research goal of scientific researchers. Therefore, it is particularly important to explore new, efficient and accurate DNA detection techniques for the analysis of a large amount of genetic in...

Claims

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Application Information

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IPC IPC(8): C12M1/34
CPCC12Q1/6834C12Q2565/519C12Q2565/607C12Q2565/629
Inventor 刘波宋彬彬张昊刘海锋刘艳格王志
Owner NANKAI UNIV
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