Preparation method of antibacterial peptide of cyclina sinensis

Abstract

The invention provides a preparation method of an antibacterial peptide of cyclina sinensis. The preparation method of the antibacterial peptide of the cyclina sinensis comprises the following steps: pretreatment: decladding the cyclina sinensis to take cyclina sinensis flesh, chopping the flesh, putting the flesh into treatment liquid for soaking, then carrying out washing and homogenating, and adding deionized water and a hydrochloric acid solution to adjust the pH value; enzymolysis: adding 0.5 to 1 percent of compound enzyme by weight into the cyclina sinensis for hydrolysis and carrying out enzyme deactivation; centrifugation: centrifugating cyclina sinensis enzymatic hydrolysate, taking supernate, adding 0.1 to 0.3 percent of a precipitation agent by weight into the supernate, and carrying out stewing and filtering; cryopreservation: the compound enzyme is prepared from the following components in parts by weight: 5 to 10 parts of pepsase, 2 to 3 parts of neutral protease, 1 to 3 parts of papain and 0.5 to 2 parts of caspase. The extracting step is simple, and the extracting rate is high; the extracted antibacterial peptide is excellent in effect, has no immunogenicity, is good in absorption effect and has a wide use range.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a preparation method of clam antibacterial peptide. Background technique [0002] At present, conventional antibiotics have corresponding drug-resistant pathogenic strains, and the problem of drug resistance of pathogenic bacteria has become an increasingly serious threat to people's health. Studies have found that antimicrobial peptides have the potential to replace traditional antibiotics, and their advantages are: high antibacterial activity, wide antibacterial spectrum, many types, wide range of options, no immunogenicity, no toxic side effects on humans and animals; good dissolution and thermal stability; it has good tolerance to high ionic strength or large pH changes, and even has the ability to resist the hydrolysis of digestive tract polypeptide enzymes. [0003] The existing technology provides a variety of methods for extracting antimicrobial...

AI Technical Summary

Problems solved by technology

[0004] The antimicrobial peptides extracted by the preparation methods provided by the prior art can be used as nutritional and functional additives in food or developed as antibiotics for drug-resistant bacteria, but the extraction rate of antimicrobial peptides needs to be improved and the absorption effect is not ideal