Preparation method of antibacterial peptide of cyclina sinensis

A technology of antimicrobial peptides and green clams, applied in anti-infective drugs, fermentation, drug combination, etc., can solve the problems of antimicrobial peptide extraction rate to be improved, absorption effect is not ideal, etc., to achieve significant enzymatic hydrolysis effect and easy control of the reaction process , a wide range of effects

Inactive Publication Date: 2017-05-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The antimicrobial peptides extracted by the preparation methods provided by the prior art can be used as nutritional and functional additives in food or developed as antibiotics for drug-resistant bacteria, but the extraction rate of antimicrobial peptides needs to be improved and the absorption effect is not ideal

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of clam antibacterial peptide, the preparation steps are as follows:

[0025] 1) Pretreatment: shell the green clams and take the meat, chop them, soak them in the treatment solution, wash them, homogenize them, add deionized water and hydrochloric acid solution to adjust the pH; there are certain impurities and Bacteria, partly adsorbed on the surface of the clam shell, and a small part enters the shell, the impurities and harmful bacteria need to be eliminated. The treatment liquid used can quickly and effectively kill the harmful bacteria and dissolve the impurities in the clam meat, avoiding the impact of impurities on the extraction The influence of the extraction purity of antimicrobial peptides.

[0026] 2) Enzymolysis: adding 0.5-1% by weight of clam compound enzyme, hydrolyzing and inactivating the enzyme; the enzymolysis method adopted has the advantages of mild reaction conditions and easy control of the reaction process, and the enzymati...

Embodiment 2

[0039] A preparation method of clam antibacterial peptide, the preferred preparation steps are as follows:

[0040] 1) Pretreatment: Shell the green clams and take the meat, chop them, soak them in the treatment solution for preferably 50 minutes, wash them, homogenate them, add deionized water and hydrochloric acid solution to adjust the pH, preferably pH = 7, add deionized water It is preferably 5 times of the volume of green clam meat;

[0041] After the green clam meat is chopped, soaking time in the treatment solution is preferably 50 minutes, and the removal effect of impurities and harmful bacteria in the green clam meat is excellent. The added deionized water is preferably 5 times the volume of green clam meat, which can fully soak the green clam meat in deionized water to achieve the purpose of removing impurities and killing harmful bacteria. Add hydrochloric acid to adjust to the preferred pH=7, which can Quickly and effectively adjust the pH value to the specified...

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PUM

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Abstract

The invention provides a preparation method of an antibacterial peptide of cyclina sinensis. The preparation method of the antibacterial peptide of the cyclina sinensis comprises the following steps: pretreatment: decladding the cyclina sinensis to take cyclina sinensis flesh, chopping the flesh, putting the flesh into treatment liquid for soaking, then carrying out washing and homogenating, and adding deionized water and a hydrochloric acid solution to adjust the pH value; enzymolysis: adding 0.5 to 1 percent of compound enzyme by weight into the cyclina sinensis for hydrolysis and carrying out enzyme deactivation; centrifugation: centrifugating cyclina sinensis enzymatic hydrolysate, taking supernate, adding 0.1 to 0.3 percent of a precipitation agent by weight into the supernate, and carrying out stewing and filtering; cryopreservation: the compound enzyme is prepared from the following components in parts by weight: 5 to 10 parts of pepsase, 2 to 3 parts of neutral protease, 1 to 3 parts of papain and 0.5 to 2 parts of caspase. The extracting step is simple, and the extracting rate is high; the extracted antibacterial peptide is excellent in effect, has no immunogenicity, is good in absorption effect and has a wide use range.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a preparation method of clam antibacterial peptide. Background technique [0002] At present, conventional antibiotics have corresponding drug-resistant pathogenic strains, and the problem of drug resistance of pathogenic bacteria has become an increasingly serious threat to people's health. Studies have found that antimicrobial peptides have the potential to replace traditional antibiotics, and their advantages are: high antibacterial activity, wide antibacterial spectrum, many types, wide range of options, no immunogenicity, no toxic side effects on humans and animals; good dissolution and thermal stability; it has good tolerance to high ionic strength or large pH changes, and even has the ability to resist the hydrolysis of digestive tract polypeptide enzymes. [0003] The existing technology provides a variety of methods for extracting antimicrobial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/30A61P31/00
CPCC12P21/06C07K1/30
Inventor 余方苗叶盛旺唐云平黄芳芳杨最素丁国芳
Owner ZHEJIANG OCEAN UNIV
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