S-100 chemiluminescence immunoassay kit and preparation method thereof

Abstract

The invention discloses an S-100 chemiluminescence immunoassay kit and a preparation method thereof. The S-100 chemiluminescence immunoassay kit comprises an S-100 monoclonal antibody coated carboxylated magnetic particles and a statin monoclonal antibody marked chemiluminescent marker. The kit can be used for completing S-100 detection by using a full-automatic chemiluminescence immunity analyzer as a detecting tool. Experiments prove that the S-100 chemiluminescence immunoassay kit has a detection sensitivity of 0.005mu g / L, which is at least improved by 10 times in comparison with a traditional S-100 detection method. The S-100 chemiluminescence immunoassay kit has a relatively high detection accuracy.

Description

technical field [0001] The invention relates to the field of in vitro detection, in particular to an S-100 chemiluminescence immunoassay kit and a preparation method thereof. Background technique [0002] Human S-100 protein is mainly concentrated in the astrocytes of the central nervous system and corresponding tumor cells. It has a low molecular weight and belongs to a group of acidic calcium-binding proteins. Because this substance can be dissolved in saturated ammonium sulfate at pH=7 solution, hence the name S-100 protein. S-100 is the most active member in the central nervous system, mainly in Schwann cells and glial cells of the nervous system, but also in Langerhans cells, chondrocytes, adrenal satellite cells and melanin cells and other non-nervous systems. [0003] At present, S-100 protein monoclonal antibody has been used clinically to detect clinical specimens to identify the origin of tumors and improve the accuracy of glioma diagnosis. S-100 protein is wide...

AI Technical Summary

Problems solved by technology

[0007] (1) Use 12×8 type, 6×8 type, 8×12 type or whole plate type 96-well special microwell plate as antigen coating equipment and reaction container, which can only be divided into 12 batches and 6 batches when used , 8 batches or the whole board can be used at one time, and independent and single-person testing cannot be carried out;

[0008] (2) There are many types of reagents used in quantitative determination, and each detection reagent must be contained in a reagent bottle, and each time a reagent is used, it is necessary to replace the suction nozzle to fill it into the microwells of the microwell plate , not only there are many types of reagent bottles, but also the operation of filling reagents is extremely cumbersome;

[0009] (3) There is a lack of corresponding labeling of the testing information. The production batch number and expiry date information of the testing reagent can only be known or known by checking the label on the outer packaging box of the kit, and the known information is not controlled during the testing process, which has great potential. large randomness;

[0010] (4) The detection reagents are in an open space during the detection process, which may easily cause cross-contamination among various reagents and affect the accuracy of the detection results;

[0011] (5) Manual operation is mostly used in the detection process, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur, and the accuracy and precision of the detection results are poor;

[0012] (6) The quantity configuration and use of the complete set of reagents for the test items are the number of items × 48 / 96 persons. If 10 items need to be tested, the configuration and use of reagents must be 10×48 / 96 persons. If Only one sample needs to detect 10 different items, and it also needs to configure reagents for 10×48 / 96 people, which has the disadvantage of not being economical and reasonable

[0017] Enzymatic chemiluminescence mainly includes horseradish peroxidase (HRP) and alkaline phosphatase, but both have certain limitations. The main disadvantage of horseradish peroxidase is: In the presence of biomolecules, it will also be blocked by H 2 o 2 Oxidation is self-luminescent, the background is relatively high, which affects the signal-to-noise ratio, the reaction kinetics is complex, there are many influencing factors, the result is not stable enough, and it is not easy to obtain a substrate with high sensitivity and long plateau period

The main disadvantages of alkaline phosphatase are: it takes a long time for the substrate to reach the plateau, and the cost of the substrate is high, resulting in high detection cost and heavy burden on patients

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