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Bacillus cereus and application thereof in oxidizing inorganic sulfide

A technology of Bacillus cereus and inorganic sulfide, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of autolysis and reduce the rate of sulfur removal

Active Publication Date: 2017-05-17
湖北宜水环保科技有限公司
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Problems solved by technology

[0008] The purpose of the present invention is to solve the problem in the prior art that sulfur oxidizing bacteria produce sulfuric acid while oxidizing sulfide in the application of removing inorganic sulfide, which inhibits the activity of sulfur oxidizing bacteria, leads to autolysis of bacteria and reduces the rate of sulfur removal, and provides One strain can tolerate the highest S 2- Bacillus cereus with a concentration of 300mg / L and its application in the removal of reduced inorganic sulfides. The strain has the characteristics of high-efficiency desulfurization in the range of pH 4.5-9.1

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  • Bacillus cereus and application thereof in oxidizing inorganic sulfide
  • Bacillus cereus and application thereof in oxidizing inorganic sulfide
  • Bacillus cereus and application thereof in oxidizing inorganic sulfide

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specific Embodiment 1

[0020] Now refer to Figure 1 to Figure 5 And specific embodiment 1 sets forth the present invention:

[0021] Specific Example 1 Source of mutant strain Bacillus cereus ZJNB-B3.

[0022] In 2015, the applicant took 250g of wet sludge deposited at the sewage discharge place of the fishmeal processing plant, and added Na 2 The method of S enriched and isolated a bacterium that can utilize inorganic sulfide, and the identification result was Bacillus cereus XJ-2 strain, with a desulfurization rate of 87.1%. The research paper on the isolation and identification of XJ-2 strain "A strain of fish meal Isolation and Identification of Strains for Removing Odor Gas from Processing Hydrogen Sulfide" was published in the Journal of Environmental Engineering in September 2015, 2015, 9(9): 4465-4470. Because the desulfurization rate of strain XJ-2 is not very high, the conditions under which XJ-2 can grow at pH 5.0 to 8.5 described in this paper are all in the natural growth state of th...

specific Embodiment 3

[0031] Specific Example 3 Study on the Physiological Oxidative Metabolism of Bacillus cereus ZJNB-B3 Strain to Different Concentrations of Sulfide.

[0032] Pick the slant culture of the two-ring test tube into 100ml of nutrient broth medium, shake and culture at 30°C for 18 hours to obtain the seed solution in the logarithmic growth phase. Inoculate the seeds in a nutrient broth medium with a pH of 7 and a total amount of 100ml at a volume ratio of 5 to 10%, and add Na 2 S aqueous solution, make the reaction initial S 2- The concentration is 100, 200, 250, 300, 400m g / L, placed at 30°C, 150r / min shaking culture for 48 hours, sampling and analyzing the content of sulfide and sulfate in the bottle every 12 hours, and the change of pH value, the results are shown in figure 2 . When the initial S 2- When the concentration is 400mg / L, the initial pH of 0h under this condition is as high as 9.3. Under strong alkaline conditions, the bacteria are partially killed and the growt...

specific Embodiment 4

[0033] Specific Example 4 Bacillus cereus ZJNB-B3 bacterial strain will S 2- Optimum initial pH value and optimum culture temperature for physiological metabolism of sulfate radicals produced by oxidation.

[0034] (1) The ZJNB-B3 strain oxidized the initial S 2- Concentration is 250mg / L can accumulate the sulfate radical physiological metabolism change that produces see image 3 , the accumulated sulfate concentration from high to low is pH7>pH6>pH5, therefore, the optimal initial pH value for the strain to oxidize sulfide to produce sulfate is 7.

[0035] (2) ZJNB-B3 strain oxidized the initial S 2- Concentration is 250mg / L can accumulate the sulfate radical physiological metabolism change that produces see Figure 4 , the accumulated sulfate concentration from high to low is 30°C > 25°C > 35°C, and the optimum culture temperature for the strain to oxidize sulfide to produce sulfate is 30°C.

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Abstract

The invention relates to bacillus cereus and application thereof in oxidizing inorganic sulfide, which belong to the technical fields of biology and environment protection. The invention discloses an inorganic sulfide-oxidizing bacterium, which is classified and named as Bacillus cereus ZJNB-B3, and preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2016337. The bacterium has a higher oxidizing ability on reduced state inorganic sulfide. The bacillus cereus provided by the invention can tolerate a natural environment with the pH ranging from 4.5 to 9.1, can be applied in desulfuration innocent treatment of natural biological desulfurization systems with mild conditions, such as malodorous hydrogen sulfide gas generated by livestock slaughter processing and feed fish meal processing.

Description

technical field [0001] The invention relates to a strain of bacillus cereus, which can be applied to the biological removal of reduced inorganic sulfides under the slightly acidic to alkaline range of pH 4.5-9.1 and aerobic conditions, and belongs to the technical field of biology and environmental protection. Background technique [0002] In the production of some food processing, a large amount of malodorous hydrogen sulfide waste gas (H 2 S is a reduced inorganic sulfide), leading to environmental pollution. For example, in my country's livestock slaughtering industry, the main component of the malodorous exhaust gas produced by the slaughtering and processing workshop is hydrogen sulfide, and the concentration can be as high as 8.0mg / m 3 . The processing of animal protein feed fish meal, because the raw materials are mostly fish with low freshness, rotten smelly fish, rotten shrimp, and leftovers from aquatic product processing plants, a large amount of hydrogen sulfid...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00C12R1/085
CPCC12P3/00C12N1/205C12R2001/085
Inventor 严小军庄荣玉娄永江沈青青何小蝶潜丽珍任雨婷
Owner 湖北宜水环保科技有限公司
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