Multielement liquid compound microorganism fertilizer and preparation method thereof

A technology for compounding microorganisms and fertilizers is applied in the field of organic fertilizers containing bacterial cultures, mycelium or other similar substances and their preparation, which can solve the problems of unbalanced nutrient content, limited application effects, and confusion in the microbial fertilizer market. , to achieve the effect of convenient management and control, saving labor costs, and saving batching time

Inactive Publication Date: 2017-05-24
胡世洋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main problems that exist are also the key to restricting the development of microbial fertilizers.
There are mainly the following points: First, the strains used in microbial fertilizers are single, and their application effects are limited
Second, the content of nutrient elements in microbial fertilizers is unbalanced, which r...

Method used

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  • Multielement liquid compound microorganism fertilizer and preparation method thereof
  • Multielement liquid compound microorganism fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Such as figure 1 As shown, the multi-element liquid composite microbial fertilizer of this embodiment is prepared according to the following steps:

[0061] (1) Steps for high-density cultivation of Bacillus megaterium:

[0062] Under aseptic conditions, use the inoculation loop to pick 5 loops of Bacillus megaterium and inoculate it into 100mL medium (300mL Erlenmeyer flask), and then perform shake flask fermentation culture (first-level seed culture). The culture condition is: culture temperature 30℃ , Fermentation time 20h, rotation speed 180r / min, pH value 6.5. The above-mentioned cultivated first-level seeds were inoculated into a 2L medium (3.7L fermentor) at a ratio of 5% to carry out the fermentation culture of Bacillus megaterium. The culture conditions were: fermentation temperature 30℃, aeration volume 1.0vvm, rotation speed 180r / min, pH value is 6.5, fermentation time is 20h, after fermentation, the fermentation broth is stored in a refrigerator at 4°C for lat...

Embodiment 2

[0077] The difference between the multi-element liquid composite microbial fertilizer of this embodiment and the embodiment 1 is:

[0078] The composition of seed and fermentation medium in step (1) includes: potassium dihydrogen phosphate (chemically pure) 4.9g, magnesium sulfate (chemically pure) 0.45g, sodium chloride (chemically pure) 4.9g, glucose (chemically pure) 9.9 g. Peptone (biochemical reagent) 9.9g, yeast extract powder (biochemical reagent) 3.9g and Tween-80 (chemically pure) 2.9g, then add 990mL purified water, stir well, sterilize at 110°C for 40min .

[0079] The composition of seed and fermentation medium in step (2) includes: potassium dihydrogen phosphate (chemically pure) 5.1g, magnesium sulfate (chemically pure) 0.55g, sodium chloride (chemically pure) 5.1g, glucose (chemically pure) 10.1 g. Peptone (biochemical reagent) 10.1g, yeast extract powder (biochemical reagent) 4.1g and Tween-80 (chemically pure) 3.1g, then add 1010mL purified water, stir well, steri...

Embodiment 3

[0082] The difference between the multi-element liquid composite microbial fertilizer of this embodiment and the embodiment 2 is:

[0083] The deployment of NKP macronutrient elements in step (4) is carried out as follows:

[0084] Weigh 85g ammonium sulfate (analytical pure), 90g potassium dihydrogen phosphate (analytical pure), 25g potassium sulfate (analytical pure), 85g diammonium hydrogen phosphate (analytical pure) and 120g urea (analytical pure), and then add 650mL for purification Water, water temperature at 60℃, stirring at 300r / min for 15min, all reagents are dissolved, pH value is 5.0~6.0, (N+P 2 O 5 +K 2 O)≥200g / L, N+P 2 O 5 +K 2 O: 8-8-4(S), the deployment is completed and ready for use.

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Abstract

The invention discloses a multielement liquid compound microorganism fertilizer and a preparation method thereof and relates to an organic fertilizer containing bacterial culture, mycelia or other analogues and a preparation method thereof. The objective of the invention is to provide the method for preparing the novel multielement liquid compound microorganism fertilizer. According to the method, a high and new synthetic technology is adopted to composite beneficial microorganisms, humic acid (organic substances) and inorganic nutritive elements to form a novel fertilizer. The number of effective living bacteria in the multielement liquid compound microorganism fertilizer is larger than or equal to 0.1 billion /ml. The multielement liquid compound microorganism fertilizer and the preparation method thereof are used for the technical field of agriculture.

Description

Technical field [0001] The present invention relates to the field of agricultural technology, in particular to an organic fertilizer containing added bacterial culture, hyphae or other similar substances and a preparation method thereof. Background technique [0002] The growth and development of crops requires the supply of various nutrients, and the source of the nutrients is mainly fertilizer. Fertilizer is the guarantee of sustainable agricultural development and the basis for increasing crop production. In the 1950s and 1960s, the use of chemical fertilizers and pesticides made great progress in the development of agriculture in our country, and at the same time brought huge benefits to farmers. However, long-term large-scale use of chemical fertilizers and pesticides will cause soil compaction; soil water holding capacity will decrease; soil fertility is imbalanced and fertility will decrease; and the ratio of input to output will decrease. What's more serious is that thes...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C12N1/20C12R1/11C12R1/10C12R1/465C05G3/80
CPCC05B7/00C05G3/80C05G5/23C12N1/20C05C3/00C05C9/00C05D1/02C05D9/02C05F11/02C05F11/08
Inventor 胡世洋王丹
Owner 胡世洋
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