Slow-release antibacterial composite film capable of guiding bone tissue regeneration and preparation method thereof

A bone tissue regeneration and composite membrane technology, which is applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as osseointegration and bone regeneration without considering the effect of bone regeneration, and achieve a firm combination

Active Publication Date: 2017-05-31
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strategies or studies are usually only used to evaluate the anti-infection ability of orthopedic implants, without considering the impact on osseointegration and bone regeneration; or orthopedic implant materials only consider the promotion of tissue cell adhesion, proliferation, differentiation, and mineralization And improve the interface osseointegration rate, bone healing speed, osseointegration strength, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]A method for preparing a slow-release antibacterial composite membrane that can guide bone tissue regeneration, comprising the following steps:

[0033] (1) Preparation of the base bionic layer: Weigh 0.5 g of nanometer γ-polyglutamic acid / hydroxyapatite powder and add it to 1 L of 0.5% cetyltrimethylammonium bromide aqueous solution, and ultrasonically disperse it for 15 minutes , to prepare a dispersion solution containing nano-calcium phosphate; the bacterial cellulose membrane is first subjected to low-temperature plasma treatment to obtain a bacterial cellulose membrane with a surface rich in oxygen polar functional groups, and then the bacterial cellulose membrane with a surface rich in oxygen polar functional groups The membrane is suspended in a dispersion solution containing nano-calcium phosphate, stirred by 100r / min magnetic force for 8h, left standing for 12h, and washed with distilled water to obtain a nano-calcium phosphate / bacterial cellulose membrane, whic...

Embodiment 2

[0043] A method for preparing a slow-release antibacterial composite membrane that can guide bone tissue regeneration, comprising the following steps:

[0044] (1) Preparation of the base biomimetic layer: Weigh 2.0 g of γ-polyglutamic acid / β-tricalcium phosphate powder and add it to 1 L of 5.0% sodium pyrophosphate aqueous solution, and ultrasonically disperse for 60 minutes to obtain nano-phosphate-containing Dispersion solution of calcium; the bacterial cellulose membrane is first subjected to low-temperature plasma treatment to obtain a bacterial cellulose membrane rich in oxygen polar functional groups on the surface, and then the bacterial cellulose membrane rich in oxygen polar functional groups on the surface is suspended on a surface containing nano-phosphoric acid In the calcium dispersion solution, 200r / min magnetic stirring for 30h, standing still for 36h, washing with distilled water to obtain nano-calcium phosphate / bacterial cellulose film, which is the base bioni...

Embodiment 3

[0054] A method for preparing a slow-release antibacterial composite membrane that can guide bone tissue regeneration, comprising the following steps:

[0055] (1) Preparation of the base bionic layer: Weigh 1.0 g of γ-polyglutamic acid / hydroxyapatite and γ-polyglutamic acid / β-tricalcium phosphate mixture (γ-polyglutamic acid / hydroxyapatite The mass ratio of limestone and γ-polyglutamic acid / β-tricalcium phosphate is 1:1) was added in 1L of 2.0% sodium orthophosphate aqueous solution with a mass fraction of 2.0%, and ultrasonically dispersed for 30 minutes to prepare a dispersion solution containing nano-calcium phosphate; The bacterial cellulose membrane is first subjected to low-temperature plasma treatment to obtain a bacterial cellulose membrane with a surface rich in oxygen polar functional groups, and then the bacterial cellulose membrane with a surface rich in oxygen polar functional groups is suspended in a dispersion solution containing nano-calcium phosphate , 150r / m...

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Abstract

The invention discloses a slow-release antibacterial composite film capable of guiding bone tissue regeneration and a preparation method thereof. The preparation method comprises the following steps: by using a bacterial cellulose membrane as a substrate, supporting nano calcium phosphate on the bacterial cellulose membrane to obtain a nano calcium phosphate / bacterial cellulose membrane, and modifying the nano calcium phosphate / bacterial cellulose membrane by using dopamine, thereby obtaining the dopamine-modified bacterial cellulose membrane; preparing gamma-polyglutamic acid / levodopa gel microspheres, and supporting an antibacterial drug to obtain a functionalized gel; and pouring the functionalized gel onto the surface of the dopamine-modified bacterial cellulose membrane, and drying to obtain the slow-release antibacterial composite film capable of guiding bone tissue regeneration. The slow-release antibacterial composite film capable of guiding bone tissue regeneration has the firmly combined interface, has certain mechanical properties, bone guiding activity and bone conduction activity, and can slowly release the antibacterial drug to prevent or treat local infection.

Description

[0001] Technical field: [0002] The invention belongs to the field of biomedical materials, and in particular relates to a slow-release antibacterial composite film capable of guiding bone tissue regeneration and a preparation method thereof. [0003] Background technique: [0004] Guided Bone Regeneration (GBR) is to cover the surface of the bone tissue in the bone defect area with a barrier membrane, separate the soft tissue from the bone tissue, and prevent the growth of epithelial cells and fibroblasts from connective tissue into the defect area. The content of osteoinductive factors in the local bone defect is relatively increased, which induces bone growth and fills the bone defect. Among them, the barrier membrane is the key to GBR technology, and its structure and performance directly affect the effect of bone regeneration. Membrane material is the core of this technology. An ideal guiding regeneration membrane should have the following characteristics: ① biocompatibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/12A61L27/18A61L27/50A61L27/54
CPCA61L27/12A61L27/18A61L27/20A61L27/50A61L27/54A61L2300/216A61L2300/252A61L2300/406A61L2430/02C08L1/02C08L77/00
Inventor 疏秀林施庆珊陈铭杰冯静黄小茉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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