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Sodium alginate and chitosan skin tissue engineering scaffold material and preparation method thereof

A technology of skin tissue engineering and sodium alginate, which is applied in the field of sodium alginate-chitosan skin tissue engineering scaffold material and its preparation, which can solve the problems of unfavorable cell adhesion, lack of cell recognition signal and poor mechanical properties of synthetic scaffold materials

Inactive Publication Date: 2013-01-23
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although synthetic polymer materials have high tensile strength and controllable degradation rate, they are far inferior to natural polymer materials in terms of biocompatibility because they lack cell recognition signals and are not conducive to cell adhesion.
Among natural polymer materials, collagen gel and collagen sponge are classic artificial skin scaffold materials, but their disadvantages are poor mechanical properties, and the tensile strength can only reach 1-4 MPa.

Method used

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  • Sodium alginate and chitosan skin tissue engineering scaffold material and preparation method thereof
  • Sodium alginate and chitosan skin tissue engineering scaffold material and preparation method thereof
  • Sodium alginate and chitosan skin tissue engineering scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A. Prepare a scaffold matrix with a total solid content of 4 wt%. The mass ratio of sodium alginate powder to chitosan powder is 5:3, and the pH value of the system is adjusted to 4-5 with dilute acetic acid (pH=1-2).

[0022] B. Preparation of interlayer by RP technology: 2 wt% sodium alginate solution was used to obtain a dense mesh interlayer with a mesh size of 1 × 1 mm and a thickness of about 0.5 mm. In 4% CaCl 2 The solution was cross-linked for 30 min to obtain alginic acid mesh interlayer.

[0023] C. Pour the above-prepared scaffold matrix solution into the petri dish with the interlayer so that it covers the interlayer of the grid. Let stand at -7°C for 12 hours, then transfer to 2°C for 4 hours. Finally, the stents were moved to a -7°C environment and left to freeze for 12 hours.

[0024] D. Put it into a freeze dryer to freeze dry, take it out and soak it in ethanol, and vacuum dry the ethanol. with 4% CaCl 2 The solution was cross-linked for 40 min, an...

Embodiment 2

[0026] A. Prepare a scaffold matrix with a total solid content of 4 wt%. The mass ratio of sodium alginate powder to chitosan powder is 5:3, and the pH value of the system is adjusted to 4-5 with dilute acetic acid (pH=1-2).

[0027] B. Preparation of interlayer by RP technology: 4 wt% sodium alginate solution was used to obtain a dense mesh interlayer with a mesh size of 1 × 1 mm and a thickness of about 0.5 mm. In 4% CaCl 2 The solution was cross-linked for 30 min to obtain alginic acid mesh interlayer.

[0028] C. Pour the above-prepared scaffold matrix solution into the petri dish with the interlayer so that it covers the interlayer of the grid. Let stand at -7°C for 12 hours, then transfer to 2°C for 4 hours. Finally, the stents were moved to a -7°C environment and left to freeze for 12 hours.

[0029] D. Put it into a freeze dryer to freeze dry, take it out and soak it in ethanol, and vacuum dry the ethanol. with 4% CaCl 2 The solution was cross-linked for 40 min, an...

Embodiment 3

[0031] A. Prepare a scaffold matrix with a total solid content of 4 wt%. The mass ratio of sodium alginate powder to chitosan powder is 5:3, and the pH value of the system is adjusted to 4-5 with dilute acetic acid (pH=1-2).

[0032] B. Preparation of interlayer by RP technology: 4 wt% sodium alginate solution was used to obtain a dense mesh interlayer with a mesh size of 2 × 2 mm and a thickness of about 0.5 mm. In 4% CaCl 2 The solution was cross-linked for 30 min to obtain alginic acid mesh interlayer.

[0033] C. Pour the above-prepared scaffold matrix solution into the petri dish with the interlayer so that it covers the interlayer of the grid. Let stand at -7°C for 12 hours, then transfer to 2°C for 4 hours. Finally, the stents were moved to a -7°C environment and left to freeze for 12 hours.

[0034] D. Put it into a freeze dryer to freeze dry, take it out and soak it in ethanol, and vacuum dry the ethanol. with 4% CaCl 2 The solution was cross-linked for 40 min, an...

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Abstract

The invention relates to a sodium alginate and chitosan skin tissue engineering scaffold material and a preparation method thereof. According to the scaffold material, sodium alginate and chitosan take CaCl2 as a crosslinking agent to form a scaffold base, and the mass ratio of the sodium alginate to the chitosan is 5:3-3:5; and a grid structure formed by the sodium alginate is crosslinked by the CaCl2 and put on a sodium alginate and chitosan scaffold base in an interlayer mode, the grid size of the sodium alginate is (1-2)*(1-2)mm<2>, and the height is 0.2 to 0.5mm. By the scaffold material, the sodium alginate and the chitosan base which can be completely degraded are taken as base materials; by combining an interlayer manufactured by a rapid prototyping (RP) technology, the mechanical strength of the sodium alginate and chitosan scaffold material can be improved; and the pore diameter of a scaffold is 100 to 150mu m, the porosity is more than 90 percent, and the tensile strength reaches 1 to 8 MPa. The scaffold material has high biocompatibility and is suitable for growth and proliferation of cells.

Description

technical field [0001] The invention relates to a scaffold material for skin tissue engineering and a preparation method thereof, in particular to a sodium alginate-chitosan skin tissue engineering scaffold material and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body and is the barrier between the human body and the external environment. It has important functions such as excretion, sensation, prevention of water evaporation, temperature regulation, and immunity. The importance of the skin to the human body is self-evident. However, in real life, skin defects are often caused by burns, trauma, diabetic chronic ulcers and other reasons. For any full-thickness skin defect larger than 4 cm in diameter, the human body will not be able to fully heal itself. In these cases, a suitable wound repair material is required to promote wound healing. Tissue-engineered skin has been used clinically for 25 years, and it has brou...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/20A61L27/60A61L27/56C08J3/24C08L5/04C08L5/08C08K3/16
Inventor 尹静波胡玎玎吴振飞刘小琨颜世峰
Owner SHANGHAI UNIV
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