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Polysaccharide derivative containing polypeptide targeting factor and preparation method thereof

A polysaccharide derivative and peptide targeting technology, applied in the field of biomedical materials, can solve the problems of destroying the activity of polypeptides and polysaccharides, destroying the activity of polypeptides and polysaccharides, and failing to achieve the expected goals, so as to achieve cheap equipment and raw materials, reduce toxic and side effects, The effect of easy operation

Active Publication Date: 2017-05-31
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since both polypeptides and polysaccharides are biologically active substances, and the properties of polypeptides and polysaccharides are different, after synthesizing polysaccharide derivatives containing polypeptide targeting factors, the activity of polypeptides and polysaccharides will often be destroyed, and the expected goals cannot be achieved. Therefore, It is of great significance to find a special and suitable method to synthesize polysaccharide derivatives containing polypeptide targeting factors, so that the activities of polypeptides and polysaccharides are not destroyed.
However, there are no relevant research reports in the prior art

Method used

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  • Polysaccharide derivative containing polypeptide targeting factor and preparation method thereof
  • Polysaccharide derivative containing polypeptide targeting factor and preparation method thereof
  • Polysaccharide derivative containing polypeptide targeting factor and preparation method thereof

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Embodiment 1

[0052] 1. Preparation method

[0053] The process flow diagram and synthetic route diagram for preparing polysaccharide derivatives containing polypeptide targeting factors in the present invention are respectively as follows figure 1 and figure 2 shown.

[0054] The specific preparation method is as follows:

[0055] (1) Weigh 0.1g of 3-dimethylaminopropylaminoglycogen derivative (DMAPA-Gly) into a dry flask, add 10mL of dehydrated dimethylformamide, and stir at room temperature at 200r / min under nitrogen protection 12 hours to dissolve.

[0056] (2) Slowly add 0.5 mL of pyridine dropwise to the solution obtained in step (1) with a syringe, stir at room temperature at 200 r / min for 10 minutes, then slowly add 0.5 mL of acrylic acid with a syringe, and stir at room temperature at 200 r / min under the protection of nitrogen to react 24 Hour.

[0057] (3) The reaction solution was dialyzed against deionized water for 1 day, and then freeze-dried to obtain a 3-dimethylaminop...

Embodiment 2

[0070] 1. Preparation method

[0071] (1) Weigh 0.4g of 3-dimethylaminopropylamino chitosan derivative into a dry flask, add 5mL of dehydrated dichloromethane, and stir at room temperature at 300r / min under nitrogen protection for 24 hours to dissolve.

[0072] (2) Slowly add 0.1 mL of triethylamine dropwise to the solution obtained in step (1) with a syringe, stir at room temperature at 300 r / min for 20 minutes, then slowly add 0.1 mL of acrylic anhydride with a syringe, under nitrogen protection, stir at room temperature at 300 r / min The reaction was stirred for 1 hour.

[0073] (3) The reaction solution was dialyzed against deionized water for 2 days, and then freeze-dried to obtain 3-dimethylaminopropylamino chitosan derivatives containing acrylic acid groups.

[0074] (4) Weigh 0.1 g of 3-dimethylaminopropylamino chitosan derivative containing acrylic acid group into a dry flask, add 10 mL of pure water, stir at 300 r / min at room temperature for 1 hour to dissolve.

[0...

Embodiment 3

[0080] 1. Preparation method

[0081] (1) Weigh 1 g of 3-dimethylaminopropylamino pullulan derivative into a dry flask, add 10 mL of dehydrated dimethylformamide, and stir at room temperature at 500 r / min for 2 hours under the protection of argon to dissolve.

[0082](2) Slowly add 1.0 mL of pyridine dropwise to the solution obtained in step (1) with a syringe, stir at room temperature at 500 r / min for 30 minutes, then slowly add 1.0 mL of methacrylic acid with a syringe, under the protection of argon, stir at room temperature at 500 r / min The reaction was stirred for 12 hours.

[0083] (3) The reaction solution was dialyzed against deionized water for 3 days, and then freeze-dried to obtain 3-dimethylaminopropylamino pullulan derivatives containing methacrylic acid groups.

[0084] (4) Weigh 0.2 g of 3-dimethylaminopropylamino pullulan derivative containing methacrylic acid group into a dry flask, add 15 mL of pure water, stir at 500 r / min at room temperature for 12 hours to...

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Abstract

The invention discloses a polysaccharide derivative containing a polypeptide targeting factor and a preparation method thereof. The preparation method comprises that a polypeptide targeting factor and a polysaccharide derivative containing a 3-dimethylaminopropylamine group as a raw material undergo a mercapto-alkene click chemical reaction at the room temperature. The preparation method has mild reaction conditions, is free of strong acid and strong alkali reagents and maximally keeps the biological activity of the polypeptide and the polysaccharide. The preparation method has simple processes, is easy to operate and needs cheap equipment and raw materials. The polysaccharide derivative containing a polypeptide targeting factor can be used as a drug or a gene carrier having performances of targeted transport to a lesion location and thus the polysaccharide derivative has a certain application prospect in the field of biomedicine.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a polysaccharide derivative containing a polypeptide targeting factor and a preparation method thereof. Background technique [0002] Polysaccharide is a kind of natural polymer composed of different sugar units connected by glycosidic bonds. It exists widely in nature, is safe and non-toxic, and has very good biocompatibility and biodegradability. Therefore, polysaccharides are used in biomedicine The field of materials has attracted much attention, showing good application prospects and research value. Polysaccharides that have been widely studied include starch glucan, cellulose, chitosan, sodium alginate, hyaluronic acid, etc. The polysaccharide chain contains active functional groups such as hydroxyl, amino, and carboxyl groups. Polysaccharide derivatives with different structures and functions can be synthesized through chemical reactions, which ca...

Claims

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Application Information

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IPC IPC(8): C07K5/09C07K14/00C07K14/655C07K1/02A61K47/36A61K47/42C12N15/87
CPCA61K47/36A61K47/42C12N15/87C07K5/0817C07K14/00C07K14/655
Inventor 吴燕琳罗嘉浩白冰杨立群张黎明
Owner SUN YAT SEN UNIV
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