Pig haemoglobin glycopeptide with anti-oxidizing activity and preparation method and application thereof

A technology of porcine hemoglobin and antioxidant activity, which is applied to the preparation method of peptides, hemoglobin/myoglobin, chemical instruments and methods, etc., can solve the problems of deep color and harm, achieve low reaction temperature, improve sensitivity, and operate process simple effect

Active Publication Date: 2017-05-31
北京鸿顺养源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Yet the reaction temperature in this technical scheme reaches 80-110 ℃, and the sugar selected is xylose, ribose, glucose, fructose, galactose, if the reaction temperature is above 100 ℃, although the reaction product has good seasoning effect and certain Antioxidant effect, but the color of the product is darker, and it is easier to produce some harmful factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 porcine hemoglobin glycopeptide

[0033] Proceed as follows:

[0034] (1) Dissolve the blood cell powder in 12 times of water at 30°C, and homogenize it at 8000-10000 rpm for 1-3 minutes with a dispersing homogenizer to obtain a hemoglobin solution, which is treated with ultrasonic waves (frequency 60kH) in an ultrasonic generator After 25 minutes, a broken-wall hemoglobin solution was obtained.

[0035] (2) The resulting broken wall hemoglobin liquid is adjusted to a temperature of 55° C.; then the mass ratio of the compound protease (alkaline protease: bromelain: neutral protease: flavor protease=2:1:2:1) to blood cell powder is 1: Add complex protease at a ratio of 100, stir, enzymatically hydrolyze at 55°C for 3.0h, then incubate at 95°C for 5min, cool to room temperature; then centrifuge at 4000g for 10min, and collect the supernatant.

[0036] (3) Using a membrane with a pore size of 10,000 Daltons to separate protein peptides wit...

Embodiment 2

[0038] The preparation of embodiment 2 porcine hemoglobin glycopeptide

[0039] (1) The blood cell powder is dissolved in 12 times of water at 30°C, homogenized with a dispersing homogenizer at 10,000 rpm for 3 minutes to obtain a hemoglobin solution, and the hemoglobin solution is treated with ultrasonic waves (frequency 50kH) in an ultrasonic generator for 25 minutes to obtain Broken hemoglobin solution.

[0040] (2) The temperature of the obtained broken-wall hemoglobin solution is adjusted to 50° C.; then the mass ratio of compound protease (alkaline protease: bromelain: neutral protease: flavor protease=1:1:2:1) to blood cell powder is 1: Add complex protease at a ratio of 150, stir, enzymatically hydrolyze at 50°C for 4.0h, then incubate at 95°C for 5min, cool to room temperature; then centrifuge at 6000g for 10min, and collect the supernatant.

[0041] (3) Using a membrane with a pore size of 10,000 Daltons to separate protein peptides with a molecular weight of less t...

Embodiment 3

[0043] The preparation of embodiment 3 porcine hemoglobin glycopeptide

[0044] Proceed as follows:

[0045] (1) The blood cell powder is dissolved in 10 times of water at 30°C, homogenized at 10,000 rpm for 3 minutes with a dispersing homogenizer to obtain a hemoglobin solution, and the hemoglobin solution is treated with ultrasonic waves (frequency 60kH) in an ultrasonic generator for 20 minutes to obtain Broken hemoglobin solution.

[0046] (2) The temperature of the obtained broken-wall hemoglobin solution is adjusted to 50° C.; then the mass ratio of compound protease (alkaline protease: bromelain: neutral protease: flavor protease=2:1:2:1) to blood cell powder is 1: Add complex protease at a ratio of 150, stir, enzymatically hydrolyze at 60°C for 4.0h, then incubate at 98°C for 3min, cool to room temperature; then centrifuge at 5000g for 15min, and collect the supernatant.

[0047] (3) Treat the supernatant obtained in step (2) by ultrafiltration, use a membrane with a p...

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Abstract

The invention relates to the field of animal protein processing, and particularly discloses pig haemoglobin glycopeptide with anti-oxidizing activity and a preparation method and application thereof. The pig hemoglobin glycopeptide has the advantages that the pig hemoglobin is used as raw material, and is pretreated by ultrasonic waves and the like, the multiple proteases are used for composite enzymatic hydrolysis under the condition of no adding of any acid and alkaline, and the membrane separating technique is adopted, so as to obtain a certain molecular weight of hemoglobin; the hemoglobin and lactose generate Maillard reaction under the milder condition, so as to develop the pig hemoglobin glycopeptide with anti-oxidizing function and better color and flavor. A simple and high-efficiency preparation method of the pig haemoglobin glycopeptide is established.

Description

technical field [0001] The invention relates to the field of animal protein processing, in particular to porcine hemoglobin glycopeptides with antioxidant activity. Background technique [0002] The protein is enzymatically hydrolyzed to obtain a polypeptide, which changes the structure of the protein, and the active functional groups in the hydrophobic region are exposed. With the cleavage of the peptide bond, the small molecule protein peptide and amino acid increase, which can provide a source of protons or electrons and maintain a high Oxidation-reduction potential, so that it has the ability to scavenge active free radicals. Oxidation is an important metabolic process for aerobic organisms, especially vertebrates and humans, but it leads to the formation of free radicals. Reactive oxygen radicals (ROS) are thought to cause oxidative stress. In food systems, lipids or proteins may be attacked by ROS and undergo oxidation processes, resulting in unpleasant taste, dark c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/805C12P21/06C07K1/34C07K1/107A23L33/18A23L27/20A23K20/147
CPCA23V2002/00C07K14/805C12P21/06A23V2200/30A23V2250/55
Inventor 徐玲罗永康孙茜胜廖国礼冯超
Owner 北京鸿顺养源生物科技有限公司
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