Strain for generating pullulan and method for producing pullulan by fermenting strain

A technology of pullulan polysaccharide and bacterial strain, applied in the field of fermentation and production of pullulan polysaccharide, can solve problems such as difficulty, difficult separation, increase of separation steps and cost, etc., and achieve simple raw materials, simple and easy method, and simplified post-processing technology Effect

Inactive Publication Date: 2017-05-31
SHIHEZI UNIVERSITY
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Aureobasidium pullulan produces melanin during the synthesis of pullulan, the melanin will be adsorbed on the pullulan molecule, whic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] 1) Take a loop of Aureobasidium pullulans HL-1 slant strain cultured at 26℃ for 48h, inoculate it into liquid seed culture medium, the filling volume is 60ml culture medium in 500ml Erlenmeyer flask, rotating speed is 220rpm, 25℃ culture for 48h to obtain seeds liquid.

[0025] 2) Inoculate the prepared seed liquid into the fermentation medium at a ratio of 10%, and fill in 60ml of medium in a 500ml triangular shaker at a speed of 220rpm, 28°C, and cultivate for 60h;

[0026] The above seed medium is: 50g / l sucrose, 5g / l yeast extract, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O;

[0027] The above fermentation medium: 74g / l glucose, 2g / l sodium nitrate, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O.

[0028] 3) Centrifuge the fermentation broth obtained in step 2) to remove the bacteria, add 2 times the volume of methanol to the filtered supernatant, stir gently, leave it for 1 hour, centrifuge to remove the supernatant, and was...

Example Embodiment

[0029] Example 2:

[0030] 1) Take a loop of Aureobasidium pullulans HL-1 slant strain cultured at 25℃ for 48h, inoculate it in liquid seed culture medium, the filling volume is 50ml culture medium in 500ml Erlenmeyer flask, rotation speed is 220rpm, 26℃ cultivate 55h to obtain seeds liquid.

[0031] 2) Inoculate the prepared seed liquid into the fermentation medium at a ratio of 10%, and fill in 60ml of medium in a 500ml triangular shaker at a speed of 220rpm, 27°C, and cultivate for 60h;

[0032] The above seed medium is: 50g / l sucrose, 5g / l yeast extract, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O;

[0033] The above fermentation medium: 100g / l sucrose, 2g / l sodium nitrate, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O.

[0034] 3) Centrifuge the fermentation broth obtained in step 2) to remove the bacteria, add 2 times the volume of methanol to the filtered supernatant, stir gently, leave it for 1 hour, centrifuge to remove the supernatant, and wash t...

Example Embodiment

[0035] Example 3:

[0036] 1) Same as Example 1.

[0037] 2) Same as Example 1, the difference is: fermentation medium: 70g / l glucose, 1.5g / l NaNO 3 , 0.5g / l sodium chloride, 4g / l KH 2 PO 4 , 0.1g / l MgSO 4 ·7H 2 O.

[0038] 3) Same as Example 1.

[0039] After the product is dried, the yield can reach 59.03g / l.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a strain for generating pullulan. The strain is a strain which is obtained from plant leaves and does not generate melanin. The classification and nomenclature of the strain is Aureobasidium pullulans HL-1 as a Latin name. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.10772. A method for producing the pullulan by fermenting the strain is simple and feasible; a product is high in conversion rate so as to facilitate later-stage separation and purification.

Description

technical field [0001] The invention relates to a high-yielding pullulan strain and a method for fermenting and producing pullulan using the strain. Background technique [0002] As an extracellular polymer synthesized by microorganisms, pullulan is easily soluble in water, safe, non-toxic, edible, and degradable. It is a multifunctional green polymer material. Its structure is composed of glucose combined into maltotriose by α-1, 4 glycosidic bonds, and the two ends are combined with other maltotriose by α-1, 6 glycosidic bonds, and so on. This unique way of connecting monomers makes it have material properties that other polysaccharides do not have: excellent plasticity, film-forming and fiber-forming capabilities, and gas and oxygen barrier capabilities. The degradable packaging material has a wide range of uses in the fields of medicine, food, cosmetics, and environmental protection. Especially in the field of pharmaceutical excipients, the hollow capsule made of pullu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12P19/10C12R1/645
CPCC12P19/10C12N1/145C12R2001/645
Inventor 张慧莉韩蓓佳吕仁杰徐志南
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap