Bacterial strain capable of rapidly degrading nitrogen in sewage, and applications of bacterial strain

A bacterial strain and sewage treatment technology, applied in the field of environmental engineering, can solve problems such as difficult nitrogen treatment, achieve broad application prospects, and shorten the start-up time

Active Publication Date: 2017-05-31
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to solve the problem that nitrogen in sewage is difficult to treat, the present invention screened and obtained a bacterial

Method used

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  • Bacterial strain capable of rapidly degrading nitrogen in sewage, and applications of bacterial strain
  • Bacterial strain capable of rapidly degrading nitrogen in sewage, and applications of bacterial strain
  • Bacterial strain capable of rapidly degrading nitrogen in sewage, and applications of bacterial strain

Examples

Experimental program
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Example Embodiment

[0039] Example 1: Screening of strains

[0040] The strain of the present invention adopts a plate dilution separation method to separate and screen strains that can degrade nitrogen under aerobic conditions from the sludge flora.

[0041] The separation and screening methods are as follows:

[0042] 1. Dilution of mixed strains: take the bottom sludge from Zibo Boshan Sewage Treatment Plant, dissolve the sludge in deionized water (1g sludge is dissolved in 200ml deionized water), and perform gradient dilution of strains .

[0043] 2. Preparation of industrial sewage samples: Take industrial sewage from Boshan Sewage Treatment Plant in Zibo City (total nitrogen content is about 100mg / L), and adjust its pH to about 7 after filtering impurities. Fill it in a triangular flask (specification: 250ml) 150ml, add 0.48g glucose (carbon to nitrogen molar ratio: 10), sterilize at 115°C for 30min.

[0044] 3. Preliminary screening of bacterial species: take 20 microliters of the bacterial soluti...

Example Embodiment

[0050] Example 2: Detection of aerobic denitrification performance of Paracoccus denitrificans DYTN-1

[0051] Prepare simulated sewage (glucose 5.3g, NaNO 3 1.5g, NaCl 3g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, H 2 O 1.0L, pH7.0~7.5), the initial concentration of nitrate nitrogen is 247mg / L, and the molar ratio of carbon to nitrogen is 10. Pick 1 ring of Paracoccus denitrificans DYTN-1 from the slope and inoculate it in 50ml nutrient broth liquid medium (peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g, distilled water 1L, pH= 7.0) In a 250ml Erlenmeyer flask, culture at 30°C, 200rpm shaking for 36h, centrifuge at 5000rpm for 10min to discard the supernatant, and collect the bacteria. Resuspend the bacteria with sterilized simulated sewage and put it in a 250ml Erlenmeyer flask containing 150ml simulated sewage. Ferment for 36h under the conditions of shaking culture at a temperature of 30℃ and 200rpm. The removal rate of nitrate n...

Example Embodiment

[0052] Example 3: Detection of heterotrophic nitrification-aerobic denitrification performance of Paracoccus denitrificans DYTN-1

[0053] Prepare simulated wastewater 1 (glucose 5.9g, (NH 4 ) 2 SO 4 1.25g, NaCl 3g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, H 2 O 1.0L, pH 7.0~7.5), the initial concentration of nitrate nitrogen is 265mg / L, and the molar ratio of carbon to nitrogen is 10. Pick 1 ring of Paracoccus denitrificans DYTN-1 from the slope and inoculate it in 50ml nutrient broth liquid medium (peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g, distilled water 1L, pH= 7.0) In a 250ml Erlenmeyer flask, culture at 30°C, 200rpm shaking for 36h, centrifuge at 5000rpm for 10min to discard the supernatant, and collect the bacteria. Resuspend the bacteria with sterilized simulated sewage 1 and put them in a 250ml Erlenmeyer flask containing 150ml simulated sewage 1, and ferment for 36h under the condition of shaking culture at 30℃ and 20...

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Abstract

The invention discloses a bacterial strain capable of rapidly degrading nitrogen in sewage, and applications of the bacterial strain, belonging to the technical field of environment engineering. According to the invention, paracoccus denitrificans DYTN-1 capable of rapidly degrading nitrogen in sewage can be obtained through screening, the inorganic nitrogen component harmful to cultured organisms in a water environment can be controlled by the bacterial strain, and the ecological environment of a cultured water body can be rapidly restored, thus creating a good water body environment for aquacultured organisms. The paracoccus denitrificans DYTN-1 can be directly applied to nitrogen removal by denitrification for nitrate-containing nitrogen waste water, and can also be applied to nitrogen removal of ammonia-nitrogen-containing waste water, thus being a bacterium with a heterotrophic nitrification-aerobic denitrification function. After the bacterium is invested and used, the starting time of the system can be shortened, and the paracoccus denitrificans DYTN-1 has wide application prospects in various waste water nitrogen removal treatment processes, and is especially suitable for biological nitrogen removal technology of synchronized nitrification and denitrification.

Description

technical field [0001] The invention relates to a bacterial strain for rapidly degrading nitrogen in sewage and an application thereof, belonging to the technical field of environmental engineering. Background technique [0002] In recent decades, people have gradually paid attention to environmental protection, and the state has stepped up efforts to prevent and control polluted water bodies. However, the pollution of rivers and lakes has not been effectively curbed for many years. The pollution load of water bodies has continued to increase, and the problem of eutrophication is still serious. Security remains at risk. [0003] Analyze from the following two aspects: [0004] First, from the perspective of point source pollution, industrial enterprises and sewage treatment plants still have problems such as tail water or reclaimed water nitrogen discharge not up to standard, imperfect discharge standards and insufficient supervision. It is urgent to develop a nitrogen remo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/20C12N1/205C12R2001/01
Inventor 邓禹卢伟强张晓娟毛银
Owner JIANGNAN UNIV
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