Recombinant cell line stably expressing CSFV e2 protein, preparation method, application, and CSFV subunit vaccine

A recombinant cell line and subunit vaccine technology, applied in the field of molecular biology and veterinary biological products, can solve the problems of increased difficulty in production process, poor folding and modification, cell lysis, etc., and achieve good biological safety and long duration , Good antigenic effect
CN106755087BActive Publication Date: 2019-02-26北京博尔柯生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京博尔柯生物科技有限公司
Publication Date
2019-02-26

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Abstract

The invention relates to a method for preparing a recombinant cell line for stably expressing a swine fever virus E2 protein. The method for preparing the recombinant cell line for stably expressing the swine fever virus E2 protein comprises the steps of providing a recombinant expression plasmid, wherein the recombinant expression plasmid contains a swine fever virus E2 protein coding sequence and a green fluorescent protein coding sequence which are effectively connected with each other; transfecting the recombinant expression plasmid and a helper plasmid into an eukaryotic cell; screening out a single cell for expressing the recombinant expression plasmid by using a fluorescence detection method and subculturing the monoclonal cell for multiple times to obtain subcultured cell clone; and detecting the expression quantity of the swine fever virus E2 protein in the cell clone and screening out the recombinant cell line which can still express the swine fever virus E2 protein after multiple subcultures, wherein the nucleotide sequence of the swine fever virus E2 protein is shown in sequence 1, and the amino acid sequence for coding the swine fever virus E2 proteinis is shown in sequence 2. The invention further relates to the recombinant cell line for stably expressing the swine fever virus E2 protein, application and a swine fever virus sub-unit vaccine.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology and veterinary biological products. Specifically, the present invention relates to a recombinant cell line stably expressing the E2 protein of classical swine fever virus and a preparation method thereof, the application of the recombinant cell line in the preparation of a subunit vaccine of classical swine fever virus, and a strain of classical swine fever containing the recombinant cell line. The virus subunit vaccine and the preparation method of the swine fever virus subunit vaccine. Background technique

[0002] Classical swine fever (CSF) is an acute and highly fatal disease of pigs caused by classical swine fever virus (CSFV). The mortality rate, the International Office of Epizootics (OIE) defines it as a disease that must be notified, and my country lists it as a first-class animal disease. Because pigs of different ages, sexes and breeds can be infected, swine fever causes hug...

Claims

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