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Method for accelerating enzymolysis saccharifying of lignocellulose by utilizing sludge protein as enzymolysis accessory ingredient

A technology of lignocellulase and lignocellulose, which is applied in the field of lignocellulose enzymatic hydrolysis and saccharification, can solve the problems of no research on enzymatic hydrolysis aids, increased input costs, and no significant increase in cellulase activity, so as to solve the energy crisis , save consumption, realize the effect of resource utilization

Inactive Publication Date: 2017-06-13
YULIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, these methods have enhanced the efficiency of enzymatic hydrolysis of lignocellulose to a certain extent, but the cost of enzymatic hydrolysis and saccharification has not been reduced. On the contrary, the addition of enzymatic hydrolysis aids has additionally increased the input cost. There was no significant increase in activity
[0005] For sludge protein, it is derived from municipal sludge and is mainly used as a foaming agent. There is no research on enzymatic hydrolysis aids yet.

Method used

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  • Method for accelerating enzymolysis saccharifying of lignocellulose by utilizing sludge protein as enzymolysis accessory ingredient
  • Method for accelerating enzymolysis saccharifying of lignocellulose by utilizing sludge protein as enzymolysis accessory ingredient
  • Method for accelerating enzymolysis saccharifying of lignocellulose by utilizing sludge protein as enzymolysis accessory ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Sampling of municipal sludge and biomass:

[0037] The municipal sludge is taken from the urban sewage treatment plant to remove the excessively bulky impurities in the sludge; the lignocellulosic biomass is selected from hybrid poplar sawdust, first take a certain amount of hybrid poplar sawdust, pick out the foreign matter, and the selected hybrid poplar sawdust Crush and sieve (40 mesh), soak the sifted sawdust powder in deionized water for 1 hour.

[0038] (2) Preparation of sludge protein:

[0039] Adjust the pH of the selected urban sludge to 9 with calcium oxide, take 300 mL of the adjusted pH sludge and place it in a 1 L reactor, and react for 20 minutes under the action of ultrasonic waves (108 W, 20.024 kHz). Then the reactant was cooled to room temperature, and centrifuged at 3000r / min for 10min. The supernatant was taken out and dried at 25°C for 5 days to obtain sludge protein powder, which was sealed in a polyethylene bag and stored at 4°C for later ...

Embodiment 2

[0046] (1) Sampling of municipal sludge and biomass:

[0047] The municipal sludge is taken from the urban sewage treatment plant to remove the excessively bulky impurities in the sludge; the lignocellulosic biomass is selected from hybrid poplar sawdust, first take a certain amount of hybrid poplar sawdust, pick out the foreign matter, and the selected hybrid poplar sawdust Crush and sieve (50 mesh), soak the sifted sawdust powder in deionized water for 2 hours.

[0048] (2) Preparation of sludge protein:

[0049] Adjust the pH of the selected urban sludge to 10.5 with calcium oxide, take 350mL of the adjusted pH sludge and place it in a 1L reactor, and react under the action of ultrasonic waves (108W, 20.024kHz) for 25min. Then the reactant was cooled to room temperature, and centrifuged at 3000r / min for 11min. The supernatant was taken out and dried at 30°C for 5 days to obtain sludge protein powder, which was sealed in a polyethylene bag and stored at 4°C for later use. ...

Embodiment 3

[0056] (1) Sampling of municipal sludge and biomass:

[0057] The municipal sludge is taken from the urban sewage treatment plant to remove the excessively bulky impurities in the sludge; the lignocellulosic biomass is selected from hybrid poplar sawdust, first take a certain amount of hybrid poplar sawdust, pick out the foreign matter, and the selected hybrid poplar sawdust Crush and sieve (60 mesh), soak the sifted sawdust powder in deionized water for 2.5 hours.

[0058] (2) Preparation of sludge protein:

[0059] Adjust the pH of the selected urban sludge to 11 with calcium oxide, take 400 mL of the adjusted pH sludge and place it in a 1 L reactor, and react for 30 minutes under the action of ultrasonic waves (108 W, 20.024 kHz). Then the reactant was cooled to room temperature, and centrifuged at 3000r / min for 12min. The supernatant was taken out and dried at 35°C for 5 days to obtain sludge protein powder, which was sealed in a polyethylene bag and stored at 4°C for la...

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Abstract

The invention relates to a method for accelerating enzymolysis saccharifying of lignocellulose by utilizing sludge protein as an enzymolysis accessory ingredient. The method comprises the following steps: crushing, screening and soaking lignocellulose biomass and separating lignocellulose biomass powder; taking municipal sludge and adjusting pH value to 9-12; performing ultrasonic treatment and then cooling to room temperature; separating supernate and drying, thereby acquiring the sludge protein powder; adding citric acid-sodium citrate buffer solution into the lignocellulose biomass powder and uniformly mixing, thereby acquiring a mixed solution; adding cellulase and sludge protein powder into the mixed solution, thereby acquiring enzymatic hydrolysate; and performing enzymatic hydrolysis reaction for 25-48h at 35-50 DEG C, thereby completing the enzymolysis saccharifying of the lignocellulose by utilizing the sludge protein as the enzymolysis accessory ingredient. According to the method provided by the invention, the sludge protein is used as the enzymolysis accessory ingredient; the non-specificity absorption of the lignocelluloses to cellulase is reduced; the dosage of the cellulase is saved; the enzymolysis saccharifying of the lignocelluloses is enhanced; the resource utilization of the sludge is realized; the efficiency is higher.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of lignocellulose enzymatic hydrolysis, and relates to a method for promoting enzymatic hydrolysis and saccharification of lignocellulose by using sludge protein as an enzymatic hydrolysis auxiliary agent. [0003] 【Background technique】 [0004] Lignocellulose is an inexpensive and abundant renewable resource. With the severe energy crisis and people's increasing attention to environmental issues, the efficient development of lignocellulosic biomass resources has been widely valued by scholars at home and abroad. Lignocellulose hydrolysis and saccharification is a key link in the process of resource utilization. Among many hydrolysis methods, enzymatic hydrolysis is favored for its thoroughness, mild conditions, less by-products, environmental protection, and low sugar loss. However, due to the complex structure and composition of lignocellulose, short lifespan, poor stability, and low activity of cellulase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 相玉琳相玉秀王立鹏高立国王若恺
Owner YULIN UNIV
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