Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paddy rice herbicide resistant protein and gene as well as application of protein and gene

A herbicide-resistant and herbicide-resistant technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of serious weed damage, no resistance, and herbicides cannot be applied, and achieve good application prospects

Active Publication Date: 2017-06-20
HUNAN HYBRID RICE RES CENT
View PDF6 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The damage of weeds in direct-seeding paddy fields is heavier than that in transplanted paddy fields, and its control is more difficult
[0003] At present, acetolactate synthase-inhibiting herbicides are widely used as a herbicide, and have a good control effect on many weeds, but ordinary rice does not have resistance to acetolactate synthase-inhibiting herbicides, which leads to such herbicides Cannot be used for weed control in paddy fields

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paddy rice herbicide resistant protein and gene as well as application of protein and gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044]First, take 5 kg of rice seeds of the Huahang 31 strain, soak the seeds in clean water for 16 hours in a 28°C constant temperature incubator, remove the seeds and control the water. The seeds were again treated with 1% (w / w) ethyl methanesulfonate in a constant temperature incubator at 28° C. for 8 hours, and the seeds were taken out and dried. Rinse the seeds with clean water, change the water 8 times, germinate and sow at 37°C, and harvest 300 kg of Huahang 31 mutagenized M1 seeds.

[0045] Then, the harvested M1 generation seeds were sown at a high density, and when they grew into 3 to 4 leaves, the field was sprayed with a concentration of 3.33% Bean Xerox (which contained 5% by weight of imazethapyr). Three imazethapyr-resistant mutant plants died. Such as figure 1 As shown, after 25 days of spraying Dou Xerox, it can be found that the mutant rice 1, 2 and 3 did not grow poorly or withered and died, and all grew well; while the wild-type rice had all died.

[004...

Embodiment 2

[0049] This embodiment is used to detect the activity of anti-herbicide protein and wild type ALS protein as shown in SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.5, and sulfonylurea herbicide, imidazolinone Class herbicides, triazolopyrimidine herbicides and salicylic acid pyrimidine herbicides are shown in SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.5 anti-herbicide protein and wild-type ALS protein Inhibition rate of in vitro activity.

[0050] After 5 g of leaves of Huahang 31 mutant plants 1, 2, 3 and wild-type Huahang 31 plants obtained in Example 1 were chopped up, 10 mL of potassium phosphate buffer solution (pH 7, containing 1 mM sodium pyruvate, 50 mM, 0.5mM magnesium chloride, 0.5mM TPP and 10μM FAD) and quartz sand were ground to obtain a ground mixed material; the ground mixed material was filtered using 8 layers of gauze to obtain a filtrate; Centrifuge at 20,000rpm for 30 minutes, take the supernatant; add ammonium sulfate to the supernatant to make it reach 50% saturation, the...

Embodiment 3

[0056] This example is used to verify that transgenic plants expressing the herbicide-resistant protein shown in SEQ ID NO.2, SEQ ID NO.3 or SEQ ID NO.5 are resistant to sulfonylurea herbicides, imidazolinone herbicides, Resistance to triazolopyrimidine and salicylic acid pyrimidine herbicides.

[0057] Use the primers shown in table 2 to SEQ ID NO.8 and SEQ ID NO.9 to amplify the herbicide-resistant protein coding genes of the 3 mutant plants in Example 1, sequence the nucleotide sequences of the 3 genes, and The three genes were constructed into the pCAMBIAI1301 vector and the Agrobacterium EHA105 transformed into the recombinant plasmid was obtained by electroporation, and the three recombinant plasmids were respectively transformed into wild-type Huahang 31 plants using the Agrobacterium-mediated transformation method. Three kinds of transgenic plants expressing herbicide-resistant proteins were obtained by PCR amplification and sequencing screening in T0 generation tissue...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a paddy rice herbicide resistant protein and gene as well as application of the protein and the gene. The herbicide resistant protein is obtained by mutating Ala179 and / or Gly628 in a paddy rice acetolactate synthase protein amino acid sequence shown in SEQ ID NO.1, or is obtained by mutating Ser627 and Va1643 in the paddy rice acetolactate synthase protein amino acid sequence shown in SEQ ID NO.1; the gene for encoding the herbicide resistant protein and the application of the herbicide resistant protein and / or the gene for encoding the herbicide resistant protein in preparation of herbicide resistant plants are provided. According to the herbicide resistant protein, the gene and the application disclosed by the invention, acetolactate synthase inhibiting herbicides can be used in a process of planting gramineous plant crops, so that the requirements on direct seeding, mixed seeding, transplantation, mechanical production and the like of gramineous plants can be met, and a good application prospect is achieved.

Description

technical field [0001] The disclosure relates to the field of biotechnology, in particular to a rice herbicide-resistant protein and gene and applications thereof. Background technique [0002] Rice is the main food crop in my country and even in the world, and is the main source of calories for nearly half of the world's population. In my country, its total area, total output and output per unit area rank first among all kinds of food crops. Due to the serious impact of weed damage on rice production in the whole country, the situation of weed control in rice fields is grim. One of the technologies of rice planting, direct-directed rice technology is welcomed by rice farmers in various rice areas in my country, especially in southern my country, for its time-saving and labor-saving features. For live fields, the problem of controlling weeds is even more prominent. The damage of weeds in direct-seeding paddy fields is heavier than that in transplanted paddy fields, and it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/10C12N15/54C12N15/82A01H5/00
CPCC12N9/1022C12N15/8205C12N15/8274C12N15/8278C12Y202/01006
Inventor 赵炳然袁定阳韶也毛毕刚袁智成胡远艺彭彦罗武中
Owner HUNAN HYBRID RICE RES CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com