A kind of biotransformation preparation method of barley aglycon

Abstract

The invention discloses a biotransformation preparation method of periplogenin. For the biotransformation preparation method, a filtrate obtained after suction filtration on fermentation liquor obtained through fermentation culture of aspergillus niger HC306 is adopted as a catalyst, cortex periplocae is adopted as a raw material, then a transformation system is constructed, conversion reaction is carried out under the constant temperature oscillating condition with the temperature being 30-35 DEG C and the rotation speed being 150-250 r / min, after the reaction is finished, the reaction liquid is filtered, filter cakes are dried, separated and purified, and thus periplogenin is obtained. In the method provided by the invention, the components of the fermentation medium are simple, and the fermentation cost is low; aspergillus niger HC306 grows rapidly, has the strong sundry bacteria contamination resistance, and is easy to culture, and the batch is stable; cortex periplocae dry powder is adopted as the raw material and is fed into the aspergillus niger HC306 crude enzyme system to be transformed, the operation of separating and extracting periplocin is saved, the separating and purifying steps of enzyme are also saved, therefore, the process is simple, and the industrialized application is easy; the highest content of periplogenin achieves 7mg / g, and the cortex periplocae dry powder is the good material for extracting periplogenin.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing barley aglycone by using microbial enzyme biotransformation method. [0003] (2) Background technology [0004] Periplogenin is a cardiac glycoside present in Periplocae Cortex (CAS No. 514-39-6, chemical structure see figure 1 ), has pharmacological effects such as cardiotonic, anti-inflammatory and anti-tumor, and is effective against a variety of tumor cells cultured in vitro, such as mouse S180 sarcoma cells, human gastric cancer SGC-7901 cells, human lung cancer A549 cells and human liver cancer HepG2 cells and other tumors The cell lines all have toxic effects [Han Yubo, Zhao Aiguo. Antitumor effect research of barley aglycone. Chinese Journal of Pediatric Hematology and Tumor, 2008,13 (1): 1-5]; Inhibitory effect, and it has a good therapeutic effect on the guinea pig psoriasis model induced by propranolol, but it has no inhibitory effect on subcutaneous connective tissue cells A9, so it...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for using Aspergillus niger (Aspergillus niger) HC306 bacterial strain to ferment and prepare crude enzyme liquid to treat Persica chinensis, and transform the glucotoxin in it into glucoside, so as to better overcome the pollution of the existing acid hydrolysis method problem, and also better overcome the problem of high enzyme cost of enzymatic hydrolysis. This process has the advantages of simple process, high conversion yield and less by-products.

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