Natural extracellular matrix biological membrane as well as preparation method and applications thereof

A technology of exogenous matrix and biofilm, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of material hydrophilicity, degradation time and mechanical strength, insufficient exposure, etc., to achieve longer degradation time in vivo, The effect of eliminating hydrophilic groups and increasing mechanical strength

Active Publication Date: 2017-06-23
BEIJING DATSING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the free hydrophilic groups of some extracellular matrix materials treated by specific processes are not fully exposed, resulting in the use of thermal crosslinking technology alone to achieve r

Method used

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  • Natural extracellular matrix biological membrane as well as preparation method and applications thereof
  • Natural extracellular matrix biological membrane as well as preparation method and applications thereof
  • Natural extracellular matrix biological membrane as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Natural extracellular matrix biofilm and preparation method thereof

[0025] The meat joint factory purchases 1 pair of fresh pig small intestines, pre-treatment and cleaning to remove intestinal chyme, and cuts into intestines with a length of about 1 meter; prepares 3L of peracetic acid solution with a concentration of 0.5%, and puts the above intestines into peroxygen Soak in acetic acid solution for 1 hour, stir once every 10 minutes; wash the above-mentioned sterilized intestinal segment 5-8 times until the pH is 6.0-7.0; use an intestinal peeling machine to peel off the intestinal tissue to obtain the small intestinal submucosa; the prepared concentration is 0.05M sodium hydroxide solution, soak the above small intestinal submucosa in alkaline solution for 10 minutes, then soak in purified water for 10 minutes, repeat 3 times; wash the above small intestinal submucosa with purified water repeatedly 10 times; clean the small intestinal submucosa Lay 4 ove...

Embodiment 2

[0026] Example 2 Natural extracellular matrix biofilm and its preparation method

[0027] The meat joint factory purchases 1 pair of fresh pig small intestines, pre-treatment and cleaning to remove intestinal chyme, and cuts into intestines with a length of about 1 meter; prepares 3L of peracetic acid solution with a concentration of 0.5%, and puts the above intestines into peroxygen Soak in acetic acid solution for 1 hour, stir once every 10 minutes; wash the above-mentioned sterilized intestinal segment 5-8 times until the pH is 6.0-7.0; use an intestinal peeling machine to peel off the intestinal tissue to obtain the small intestinal submucosa; the prepared concentration is 0.25% sodium dodecylsulfonate (SDS) solution, soak the above small intestinal submucosa in SDS solution for 10 minutes, repeat 4 times; wash the above small intestinal submucosa with purified water for 10 times; wash the small intestinal submucosa Lay 4 overlapping layers, clamp the clamps, and freeze-dr...

Embodiment 3

[0028] Example 3 Natural extracellular matrix biofilm and its preparation method

[0029] The meat joint factory purchases 1 pair of fresh pig small intestines, pre-treatment and cleaning to remove intestinal chyme, and cuts into intestines with a length of about 1 meter; prepares 3L of peracetic acid solution with a concentration of 0.5%, and puts the above intestines into peroxygen Soak in acetic acid solution for 1 hour, stir once every 10 minutes; wash the above-mentioned sterilized intestinal segment 5-8 times until the pH is 6.0-7.0; use an intestinal peeling machine to peel off the intestinal tissue to obtain the small intestinal submucosa; the prepared concentration is 0.05M sodium hydroxide solution, soak the above small intestinal submucosa in alkaline solution for 10 minutes, then soak in purified water for 10 minutes, repeat 3 times; wash the above small intestinal submucosa with purified water repeatedly 10 times; clean the small intestinal submucosa Lay 4 overlap...

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Abstract

The invention provides a natural extracellular matrix biological membrane. The natural extracellular matrix biological membrane is prepared through the steps that natural extracellular matrix after antigen removal is taken as a material, 2-10 layers of monolayer materials with the same specification are placed in a staggered or parallel stacking manner, all the layers attach to each other tightly by adopting clamps, freeze-drying treatment is carried out under the vacuum condition, then the materials are placed in a vacuum oven to be subjected to dehydrothermal treatment, and finally, the extracellular matrix biological membrane is subjected to cobalt-60 irradiation or ethylene oxide sterilization. The natural extracellular matrix material is subjected to a series of antigen removal treatment, thus the biological membrane matrix material with a certain three-dimensional space structure is obtained, the biological membrane matrix material is then subjected to freeze-drying and dehydrothermal treatment, so that the biological membrane matrix material is subjected to intramolecular dehydration crosslinking, the spatial structure is reinforced, the obtained material has high rehydration stability, and the surgical operation is enabled to be more convenient. Meanwhile, the material has long degradation time in the body, the hydrophobicity and mechanical strength are increased, and thus the natural extracellular matrix biological membrane has great significance in isolating the anti-adhesion stress tissue with long repairing period.

Description

technical field [0001] The invention relates to the field of medical biomaterials, in particular to a natural extracellular matrix biofilm and its preparation method and application. Background technique [0002] Extracellular matrix (ECM) materials are often used to make various surgical biological mesh, such as dura mater mesh, hernia mesh, pleural mesh, urethral mesh and so on. Commonly used extracellular matrix materials include dermal matrix, small intestinal submucosa, bladder submucosa, and pericardium. These materials are mainly composed of collagen fibers, rich in bioactive factors and have a special three-dimensional pore structure. Compared with other biomaterials synthesized by polymers, they have better biocompatibility and promote tissue repair and regeneration. However, biological patches made of natural extracellular matrix materials are too soft and have poor mechanical properties, which brings a lot of inconvenience to surgical operations; in the repair of...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56A61L27/58
CPCA61L27/3633A61L27/3683A61L27/56A61L27/58A61L2430/40
Inventor 刘伟李智峰张玉兰李湘杰李次会
Owner BEIJING DATSING BIO TECH
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