Natural extracellular matrix biological membrane as well as preparation method and applications thereof

Abstract

The invention provides a natural extracellular matrix biological membrane. The natural extracellular matrix biological membrane is prepared through the steps that natural extracellular matrix after antigen removal is taken as a material, 2-10 layers of monolayer materials with the same specification are placed in a staggered or parallel stacking manner, all the layers attach to each other tightly by adopting clamps, freeze-drying treatment is carried out under the vacuum condition, then the materials are placed in a vacuum oven to be subjected to dehydrothermal treatment, and finally, the extracellular matrix biological membrane is subjected to cobalt-60 irradiation or ethylene oxide sterilization. The natural extracellular matrix material is subjected to a series of antigen removal treatment, thus the biological membrane matrix material with a certain three-dimensional space structure is obtained, the biological membrane matrix material is then subjected to freeze-drying and dehydrothermal treatment, so that the biological membrane matrix material is subjected to intramolecular dehydration crosslinking, the spatial structure is reinforced, the obtained material has high rehydration stability, and the surgical operation is enabled to be more convenient. Meanwhile, the material has long degradation time in the body, the hydrophobicity and mechanical strength are increased, and thus the natural extracellular matrix biological membrane has great significance in isolating the anti-adhesion stress tissue with long repairing period.

Description

technical field [0001] The invention relates to the field of medical biomaterials, in particular to a natural extracellular matrix biofilm and its preparation method and application. Background technique [0002] Extracellular matrix (ECM) materials are often used to make various surgical biological mesh, such as dura mater mesh, hernia mesh, pleural mesh, urethral mesh and so on. Commonly used extracellular matrix materials include dermal matrix, small intestinal submucosa, bladder submucosa, and pericardium. These materials are mainly composed of collagen fibers, rich in bioactive factors and have a special three-dimensional pore structure. Compared with other biomaterials synthesized by polymers, they have better biocompatibility and promote tissue repair and regeneration. However, biological patches made of natural extracellular matrix materials are too soft and have poor mechanical properties, which brings a lot of inconvenience to surgical operations; in the repair of...

AI Technical Summary

Problems solved by technology

However, the free hydrophilic groups of some extracellular matrix materials treated by specific processes are not fully exposed, resulting in the use of thermal crosslinking technology alone to achieve reinforcement between layers of materials to a certain extent, while the thermal crosslinking of materials after heat treatment Hydrophilicity, degradation time and mechanical strength have little effect

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