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Preparation method of feed mycotoxin adsorbent

A mycotoxin adsorption and feed technology, applied in animal feed, additional food elements, applications, etc., can solve problems that have not been reported, and achieve the effect of simple production method, good social and economic benefits, and easy operation

Inactive Publication Date: 2017-07-04
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Konjac glucomannan is mainly derived from the extract of the herbaceous plant konjac. It is a water-soluble ionic natural polysaccharide. It has good preventive and therapeutic effects on hypertension, diabetes, obesity, constipation, etc., and has certain anti-cancer effects Therefore, it is widely used in food, medicine, chemical industry, textile, environmental protection, polymer materials and other fields, but there is no report on the esterification of glucomannan derived from konjac and its application as a feed mycotoxin adsorbent.

Method used

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  • Preparation method of feed mycotoxin adsorbent
  • Preparation method of feed mycotoxin adsorbent
  • Preparation method of feed mycotoxin adsorbent

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: Sodium hexametaphosphate is dissolved in equal-quality distilled water, according to the mass ratio of sodium hexametaphosphate and konjac glucomannan in konjac fine powder is 1:6.5 to take konjac glucomannan and add hexameta In the sodium phosphate solution, stir and mix well. After reacting for 130 minutes at pH 3.0 and reaction temperature 75°C, the product was washed with 60% ethanol three times, then washed and dehydrated with absolute ethanol once, dried at 60°C for 24 hours, and then used Grinding with a star ball mill, then adding 60% ethanol to wash the product for 7 times at a mass ratio of 1:5 with 60% ethanol to obtain esterified konjac glucomannan;

Embodiment 2

[0045] Example 2: Characterize the esterified konjac glucomannan prepared by the sodium hexametaphosphate method, and deduce its chemical structure. Proceed as follows:

[0046] Step 1. IR Spectrum Test

[0047] The sample is dried, using the method of pressing the sample and potassium bromide, using the FT-TR spectrometer, at 400~4000cm -1 Interval scanning, measuring the infrared spectrum of konjac glucomannan and esterified konjac glucomannan;

[0048] Step 2. Gel Chromatography Conditions

[0049]Waters Ultrahydrogel Linear water-soluble gel chromatography column (300 mm×7.8 mm), the mobile phase is water, the flow rate is 1 mL / min, the gain is 1 db, the temperature of the drift tube is 80 °C, the temperature of the nebulizer is 30 °C, and the nitrogen pressure is 30 psi , the injection volume is 20 μL. Add dextran and mobile phase to prepare a 1 mg / mL standard solution, and then filter through a 0.22 μm aqueous phase filter membrane and inject 20 μL of the sample. Ko...

Embodiment 3

[0076]Embodiment 3: Synthesis of esterified konjac glucomannan by sodium hexametaphosphate method, according to aflatoxin, deoxynivalenol, zearalenone and fumonisin B1 mass ratio is 2000: 1 dissolved in water, in The temperature was 37° C., and the reaction was carried out for 8 hours in a shaker with a rotational speed of 300 r / m. After the reaction, each group was centrifuged at 3000r / m for 10min, the supernatant was taken and diluted, and the contents of aflatoxin, vomitoxin, zearalenone and fumonisin B1 were detected according to the instructions of the mycotoxin enzyme-linked immunosorbent assay kit. . The adsorption capacity of esterified konjac glucomannan synthesized by sodium hexametaphosphate method to aflatoxin, vomitoxin, zearalenone and fumonisin B1 reached 400.30 μg / g, 182.64 μg / g, 398.19 μg / g and 356.45 μg / g;

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Abstract

The invention designs a preparation method of a feed mycotoxin adsorbent. A main component namely konjac glucomannan of konjac refined powder is subjected to esterifying modification with sodium hexametaphosphate, so that esterified konjac glucomannan is synthetized, and the prepared esterified konjac glucomannan is subjected to characterization, so that the chemical structure of the esterified konjac glucomannan is deduced. According to the preparation method disclosed by the invention, further the synthetized esterified konjac glucomannan is used as the feed mycotoxin adsorbent, and common mycotoxin is subjected to adsorption research. Compared with the prior art, the preparation method disclosed by the invention is simple and convenient to operate, can be applied to large-scale production, and has favorable social and economic benefits; and the esterified konjac glucomannan is used as the feed mycotoxin adsorbent, the adsorption effect of the esterified konjac glucomannan is obviously better than that of an adsorbent namely mycosorb which is frequently used at present, and the preparation method has important significance on an application of plant source esterified glucomannan in feed additives.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a preparation method of a feed mycotoxin adsorbent. Background technique [0002] Mycotoxin poisoning can cause animal growth retardation, decreased immunity, and organ dysfunction; lead to acute and chronic poisoning death of humans and animals; in addition, mycotoxins have teratogenic, carcinogenic, and mutagenic effects on animals, causing huge damage to the breeding industry and human health. threat, so the detoxification of mycotoxins is imperative. At present, the application of esterified glucomannan as a mycotoxin adsorbent is becoming more and more extensive. The esterified glucomannan used in the market is based on the mannan extracted from the cell wall of yeast culture and modified by esterification. Synthetic mycotoxin adsorbent - Mycosuction is a widely used mycotoxin antidote because it is safe and non-toxic, has strong ability to adsorb mycotoxins, and has a wide ra...

Claims

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Application Information

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IPC IPC(8): A23K20/163C08B37/02
CPCC08B37/009
Inventor 朱连勤朱风华陈甫
Owner QINGDAO AGRI UNIV
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