A kit for marine mollusk primary cell separation and culture and uses thereof

A technology for marine molluscs and primary cells, applied in animal cells, invertebrate cells, cell dissociation methods, etc., can solve the slow progress of marine mollusc cell culture work, and there is no universal method for marine mollusk cell culture , culture medium and kits, etc., to reduce the probability of microbial contamination, ensure high activity, low pollution, and low microbial contamination rate
CN106916779AActive Publication Date: 2017-07-04XIAMEN MEDICAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN MEDICAL COLLEGE
Publication Date
2017-07-04

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Abstract

The invention provides a kit for marine mollusk primary cell separation and culture and uses thereof, and relates to marine mollusk cell culture. The kit is provided with a packing box, washing liquid I bottles, washing liquid II bottles, washing liquid III bottles, a solution A bottle, a solution B bottle, a solution C bottle and an operating instruction. The washing liquid I bottles, the washing liquid II bottles, the washing liquid III bottles, the solution A bottle, the solution B bottle, the solution C bottle and the operating instruction are in the packing box. The kit is applied in sterile separation and in-vitro primary culture of a plurality of somatic cells of a plurality of marine mollusks. Through optimizing conditions for separation and primary culture of marine mollusk cells, proper conditions for separation and primary culture of the marine mollusk cells are acquired. A problem that rapid construction of marine mollusk cell rapid separation and primary culture sterile culture systems cannot be achieved in the prior art is overcome. A method for marine mollusk primary cell rapid separation and primary cell sterile culture is established. The kit for marine mollusk primary cell separation and culture is provided.
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Description

technical field

[0001] The invention relates to marine mollusc cell culture, in particular to a marine mollusc primary cell isolation and culture kit and application thereof. Background technique

[0002] Cell culture is one of the important technologies and commonly used methods in modern bioengineering technology. It is widely used in research fields such as cell metabolism, gene transcription, protein expression, cell environment emergency and immune mechanism, drug screening and biological drug expression and production. In the research of plants, mammals, fish and insects, cell and tissue culture has become an indispensable research method, especially in related gene expression regulation mechanisms, cell signal transduction pathways, cellular environmental stress mechanisms, and genetic Cell culture has become an irreplaceable means for mutation research and other aspects, and it is also an important technology to promote the rapid development of modern biotechnology. ...

Claims

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