Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of phenazine-1-carboxamide modified compound 18-3 in inhibition of strawberry gray mould

A technology of botrytis cinerea and formamide, applied in the application, fungicide, organic chemistry and other directions, can solve the problem of weak bacteriostatic effect, etc., and achieve the effect of strong inhibition effect, good inhibition effect and difficult drug resistance

Active Publication Date: 2017-07-14
HUNAN AGRICULTURAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our research group has studied phenazine-1-carboxamide, which has antibacterial effect, but its antibacterial effect is relatively weak. Therefore, our research group used phenazine-1-carboxamide as the parent, and obtained phenazine through structural modification. -1-formamide modification 18-3

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of phenazine-1-carboxamide modified compound 18-3 in inhibition of strawberry gray mould
  • Application of phenazine-1-carboxamide modified compound 18-3 in inhibition of strawberry gray mould
  • Application of phenazine-1-carboxamide modified compound 18-3 in inhibition of strawberry gray mould

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation of phenazine-1-carboxamide modified compound 18-3

[0015] The synthetic method of modified compound 18-3: hydrolyze phenazine-1-carboxamide under acidic conditions to generate phenazine-1-carboxylic acid; take 6.2 mmol phenazine-1-carboxylic acid (1.4179 g), 6.2 mmol 4- Phenylbutylamine (1ml), 12.4mmol tris(trifluoroethoxy)boron (2.675ml), and 12.4ml acetonitrile were added to a 25ml round-bottomed flask, stirred magnetically, and reacted at 80°C for 24h; after the reaction solution was cooled, 20ml of DCM and 3 ml of water; then add 930 mg of Amberlyst A-26 (OH), Amberlyst 15, and AmberliteIRA743 resin in turn, and stir at room temperature for 30 min; then add anhydrous magnesium sulfate powder to remove water, filter, and use dry DCM to wash solid magnesium sulfate 3 times, Rotary evaporation to obtain the modified product 18-3 with a yield of 52.64%.

Embodiment 2

[0016] Example 2 Structure identification of phenazine-1-carboxamide modified compound 18-3

[0017] By hydrogen spectrum (1H NMR (500MHz, CDCl3) δ11.04 (s, 54H), 9.05–8.86 (m, 56H), 8.52–7.66 (m, 363H), 7.29 (s, 10H), 7.33–7.14 (m ,15H),4.03–3.44(m,255H),3.41(d,J=3.3Hz,163H),3.35(t,J=4.6Hz,13H),2.67(s,4H),2.33(d,J= 13.9Hz, 4H), 2.18–2.01 (m, 122H), 1.83 (dd, J=12.6, 6.1Hz, 4H), 1.48–1.14 (m, 107H), 1.74–0.78 (m, 275H), 1.53–0.78 (m, 253H), 1.00 (ddd, J=18.6, 14.5, 7.3Hz, 26H), 0.93–0.50 (m, 119H), -0.00 (s, 14H).), carbon spectrum (13C NMR (126MHz, CDCl3) )δ164.77(s), 143.41(s), 142.91(s), 141.33(s), 140.84(s), 135.35(s), 133.52(s), 131.60(s), 131.00(s), 130.09( s), 129.69(s), 129.21(s), 129.02(s), 77.32(s), 77.07(s), 76.81(s), 70.94(s), 58.80(s), 37.46(s), 29.52( s).) and mass spectrum (molecular weight: 295.20, figure 1 ) The compound synthesized by detection example 1 is consistent with the expected target, and its molecular formula is C 23 H 21 N 3 O, the structura...

Embodiment 3

[0020] (1) Instruments and related materials

[0021] YJ-VS-2 ultra-clean workbench (Wuxi Yijing Purification Equipment Co., Ltd.), LDZX-30E sterilizer (Shanghai Shen'an Medical Equipment Factory), MPJ-250 incubator (Shanghai Senxin Experimental Instrument Co., Ltd.) , QHX-400B-III type light incubator (Shanghai Xinmiao Medical Equipment Manufacturing Co., Ltd.), electric furnace (Beijing Zhongxing Weiye Instrument Co., Ltd.), etc., as well as measuring cylinders, glass rods, beakers, triangular flasks, rulers, pencils, label paper, Parafilm, picking needle, volumetric flask, inoculation needle, ph-101 watering can, hole punch, petri dish, kitchen knife, gauze.

[0022] ⑵ medicine

[0023] The original drug of 93% phenazine-1-carboxamide modified product 18-3 (hereinafter referred to as modified product 18-3) prepared in Example 1; agar, glucose, acetone and Tween 80 were purchased from Sinopharm Group.

[0024] (3) strain

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an application of a phenazine-1-carboxamide modified compound 18-3 in the inhibition of strawberry gray mould. The phenazine-1-carboxamide modified compound 18-3 is obtained through structure modification of phenazine-1-carboxamide used as a parent substance. The inhibition effect of the modified compound 18-3 on the strawberry gray mould is detected through adopting a growth rate technology, and a result shows that the modified compound 18-3 has an inhibitory effect on the strawberry gray mold, and has a higher strawberry gray mould inhibition effect than the parent substance phenazine-1-carboxamide, so the modified compound 18-3 can be used as a lead compound of new pesticides for controlling the strawberry gray mould.

Description

technical field [0001] The invention belongs to the field of pesticide creation, in particular to the application of phenazine-1-carboxamide modified compound 18-3 in inhibiting Botrytis cinerea. Background technique [0002] Strawberry is Rosaceae Strawberry plant, rich in nutritional value, known as the "Queen of Fruits", rich in vitamin C, vitamin A, vitamin E, vitamin PP, vitamin B1, vitamin B2, carotene, tannic acid, asparagus Nutrients such as amino acid, copper, strawberry amine, pectin, cellulose, folic acid, iron, calcium, ellagic acid and anthocyanins are deeply loved by consumers. Strawberries are often affected by external environmental conditions during the growth process, such as temperature, humidity, light, terrain, diseases, etc. Among them, strawberry gray mold is an important disease during the growth period of strawberries, and the annual economic loss caused by this disease can reach 30%. -60%, even aborted. [0003] At present, the methods of preventi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/60A01P3/00C07D241/46
CPCA01N43/60C07D241/46
Inventor 薛进张亚廖晓兰刘双清刘青刘妍
Owner HUNAN AGRICULTURAL UNIV