Plant-derived pesticide preparation for preventing and controlling paddy rice blast and preparation method thereof

A technology of botanical pesticides and rice blast, which is applied in the field of microorganisms, can solve the problems of lack of single use and uneven ability of actinomycetes, and achieve high control efficiency and yield increase rate, less drug resistance, and no toxic side effects.

Active Publication Date: 2017-07-14
湖南格灵科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Using live actinomycetes resistant to blast fungus as a pesticide preparation, although it can avoid the hidden dangers of chemical pesticides with its safer biological metabolites, it can also use the antagonistic correspondence between different actinomycetes and pathogenic bacteria to reduce blast disease. Due to the impact of the variety and variability of pathogens, the ability of the screened actinomycetes varies, and there is still a lack of single use, and the control effect needs to be further strengthened

Method used

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  • Plant-derived pesticide preparation for preventing and controlling paddy rice blast and preparation method thereof
  • Plant-derived pesticide preparation for preventing and controlling paddy rice blast and preparation method thereof
  • Plant-derived pesticide preparation for preventing and controlling paddy rice blast and preparation method thereof

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Effect test

Embodiment 1

[0034] Embodiment 1: preparation of Chinese medicine extract of the present invention

[0035] Take Coptis chinensis, Anemarrhena anemarrhena, Moutan cortex, Sichuan cinnamon bark, Peucedanum chinensis, cat's claw, and Acanthopanax 20g each, pulverize, mix, add 100ml of 95% ethanol, soak at room temperature for 48h, filter with 3 layers of gauze, and the filtrate is 4000r / min After centrifugation for 15 min, the supernatant was taken and evaporated to dryness at 40° C., and the residue was dissolved in 20 ml of 50% ethanol to obtain the traditional Chinese medicine extract.

Embodiment 2

[0036] Embodiment 2: Preparation of botanical pesticide preparation of the present invention

[0037] 1. Preparation of spore suspension of actinomycetes resistant to blast fungus

[0038] Step 1. Inoculate actinomycetes on the slant of the solid medium of the test tube. The medium formula is: 200g of potatoes, 20g of glucose, 20g of agar, 1000mL of water, pH value 7.2, cultivated at 28°C for 7 days to obtain the test tube species;

[0039] Step 2, prepare liquid seed medium first, the liquid seed medium formula is yeast extract 4g, glucose 4g, malt extract 10g, agar 20g, water 1000mL, pH value 7.2; Fill 100mL liquid medium in each Erlenmeyer flask, Sterilize at 120°C for 30 minutes, insert 1cm after cooling 2 The test-tube species was shaken and cultivated at 28°C for 7 days at a rotation speed of 200r / min to obtain seed liquid;

[0040] Step 3. Prepare liquid fermentation medium first. The formula of liquid fermentation medium is 20g of soybean powder, 10g of sucrose, 5g o...

Embodiment 3

[0043] Embodiment 3: plate antagonistic antibacterial test

[0044] Treatment 1: using the plate confrontation method, inoculate activated actinomycetes on a PDA medium plate with a diameter of 90 mm, 30 mm from the center of the petri dish, and then inoculate Magnaporthe grisea at the center of the petri dish. Seal the petri dish with a parafilm, and incubate it upside down in a mold incubator at 28°C for more than 7 days. Repeat each treatment 3 times, and use no actinomycetes as the CK control, and observe the experimental results after 7 days. The fungal colony diameter was measured and the fungal mycelial growth inhibition rate was calculated.

[0045] Treatment 2: The traditional Chinese medicine extract was prepared into a 50mg / ml drug-carrying medium, except that the medium was different, and other steps were the same as treatment 1.

[0046] The inhibition rate was calculated according to the following formula:

[0047] Mycelial growth inhibition rate% = (average di...

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Abstract

The invention relates to the technical field of microorganisms and discloses a plant-derived pesticide preparation for preventing and controlling paddy rice blast and a preparation method thereof. The plant-derived pesticide preparation is composed of a spore suspension of actinomycetes resisting Magnaporthe oryzae and traditional Chinese medicine extract. The traditional Chinese medicine extract is mixed extract of coptis root, rhizoma anemarrhenae, moutan bark, bark of Cinnamomum wilsonii Gamble, radix peucedani, ternate buttercup root and acanthopanax root. The invention provides traditional Chinese medicine extract for improving actinomycete prevention effects on Magnaporthe oryzae. Through combination of the traditional Chinese medicine extract and actinomycetes resisting Magnaporthe oryzae, antagonistic actinomycete prevention effects are improved, a chemical pesticide is avoided, environmental friendliness is guaranteed, and toxic and side effects are avoided. Compared with actinomycetes, the plant-derived pesticide preparation prevents pathogen resistance to pesticide.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a botanical pesticide preparation for preventing and controlling rice blast and a preparation method thereof. Background technique [0002] Rice blast (Blast Fungus), also known as rice fever, is one of the most serious rice diseases in the world. The disease can cause harm to rice all year round, and its harm spreads all over the various parts of rice, including seedling rice blast, leaf blast, leaf pillow blast, node rice blast, ear neck blast, branch blast and grain blast. Generally, rice production is reduced by 10% to 20%. Taking my country as an example, since the 1990s, the annual occurrence area of ​​the disease has been 3.8 million hectares 2 Above, the annual loss of rice reaches hundreds of millions of kilograms. [0003] At present, chemical pesticides are one of the main measures to prevent and control rice blast. However, large-scale and long-term use of c...

Claims

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Application Information

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IPC IPC(8): A01N65/42A01N65/32A01N65/10A01N65/08A01P3/00C12N1/20C12R1/04
CPCA01N65/00A01N65/08A01N65/10A01N65/32A01N65/42C12N1/20C12N1/205C12R2001/04
Inventor 钟积东谭志奇周艳
Owner 湖南格灵科技有限公司
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