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Stenotrophomonas acidaminiphila and application thereof

A technology of Stenotrophomonas and culture medium, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of poor paint resistance, poor solvent resistance, high viscosity, etc., achieve good tolerance and improve adaptability , good degradation effect

Active Publication Date: 2017-07-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pigment Red 23 is a color phenol-based azo organic pigment, which is dark blue light red, with a light fastness of grade 3 and a relative density of 1.47g / cm 3 , small particle size, transparent type, high viscosity, poor solvent resistance, resulting in crystallization in packaging printing ink
It can be used for fabric printing, NC-type and water-based printing ink, and can also be used for paint coloring, but the performance of overcoat resistance is slightly poor

Method used

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  • Stenotrophomonas acidaminiphila and application thereof
  • Stenotrophomonas acidaminiphila and application thereof
  • Stenotrophomonas acidaminiphila and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Isolation of strains

[0036] (1) Collect the activated sludge from the sewage treatment plant and put it in a glass acclimation device with a length of 31.2cm, a width of 22.0cm and a height of 22.5cm, and use an air compressor without adding nutrients and without exchange of water in and out. Intermittent aeration for 3 days. Then change the water, discharge about 10L of mud-water mixture in the device, add nutrient solution and carry out uninterrupted aeration. After cultivating for 10 days, add Pigment Red 23 solution while adding nutrient solution, keep the concentration of Pigment Red 23 in the device at about 10 mg / L, and perform domestication according to the SBR process (water intake, aeration, standing, draining, idle) Cultivate, change the water every other day, and cultivate and acclimatize for 30 days. Preparation of culture medium: 400mg / L glucose, 80mg / L anhydrous sodium acetate, 125mg / L NaHCO 3 , 4.75mg / L KCl, 2.5mg / L anhydrous CaCl 2 , 24mg / L KH ...

Embodiment 2

[0049] Inoculate the strain into Pigment Red 23 decolorization medium, measure its OD600 and absorbance at 579nm every 2 hours, use the uninoculated decolorization medium as a blank control, draw the growth curve and pigment decolorization curve of the bacteria, and determine the best The decolorization time is 18h. Select carbon source, nitrogen source, inoculation amount, temperature, pH, salinity, study the optimal conditions of a single factor, and judge the influence of each factor on the growth of the strain and the decolorization effect of the pigment. Different influence gradients are set for each factor. When studying the influence of a certain factor, only different influence levels are set for this factor, and other factors remain unchanged. All experiments were set up in three parallel experiments.

[0050] The results showed that the most suitable decolorization conditions for Stenotrophomonas acidaminiphila SP-3 were yeast powder as the nutrient source, the inoc...

Embodiment 3

[0071] Take 0.75g of sodium alginate in 50mL of sterile water, and make a colloidal solution after being completely dissolved under heating conditions, and then cool to 30°C. Under sterile conditions, mix 3mL of the bacterial suspension with the colloidal solution evenly. Use a 1mL syringe to quickly drop the mixture into the continuously stirring FeCl 3 In the solution, uniform and regular pellets are formed, and the pellets are soaked in FeCl 3 solution, and placed in a refrigerator at 4°C for 24 hours. Wash the cured and cross-linked pellets twice with distilled water to obtain immobilized microbial pellets, such as Figure 9 shown.

[0072] In the optimal nutrient source concentration is yeast powder, 1g yeast powder, 0.1g / L CaCl 2 , 0.5g / L MgSO 4 ·7H 2 O, 1g / LKH 2 PO 4 , 1g / L Na 2 HPO 4 , 100mg / L Pigment Red 23, 1L of distilled water to prepare decolorization medium, pH=6.5. Under sterile conditions, aliquot 100mL of decolorization medium into 250mL sterilized ...

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Abstract

The invention relates to stenotrophomonas acidaminiphila and application thereof. The preservation number of the stenotrophomonas acidaminiphila SP-3 is CGMCC No.13711, a nucleotide sequences is shown as SEQ ID NO.1. The stenotrophomonas acidaminiphila is used for decoloration and degradation of pigment red 23 wastewater. The azo dye wastewater degradation effect of the stenotrophomonas acidaminiphila is good, an application method is simple, the cost is low, and the stenotrophomonas acidaminiphila has a very good application prospect.

Description

technical field [0001] The invention belongs to the field of dye decolorization and degradation, and in particular relates to microacidophilic stenotrophomonas and its application. Background technique [0002] Dyes, as an organic compound, can be dissolved in water and other media to dye fibers into various stable colors. The world has an annual output of about 800,000 tons of dyes, of which China's annual output is about 150,000 tons, ranking among the top in the world. The discharge of dyes first causes changes in the chromaticity of the water body, even if there is a small amount of dyes, it will cause a large change in the chromaticity of the water body, which not only affects the transparency and solubility of the water body, but also poses a threat to the survival of aquatic organisms. The dyes used in the printing and dyeing industry can be divided into various types according to their chemical structures. Among them, azo dyes are widely used and the largest amount ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C02F3/34C12R1/01
CPCC02F3/34C02F2101/308C12N11/10C12N1/205C12R2001/02
Inventor 赵晓祥孙璐璐周月白恒程晨
Owner DONGHUA UNIV
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