Stenotrophomonas acidaminiphila and application thereof

A technology of Stenotrophomonas and culture medium, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of poor paint resistance, poor solvent resistance, high viscosity, etc., achieve good tolerance and improve adaptability , good degradation effect

Active Publication Date: 2017-07-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pigment Red 23 is a color phenol-based azo organic pigment, which is dark blue light red, with a light fastness of grade 3 and a relative density of 1.47g/cm 3 , small particle size, transparent type, high viscosity, poor solvent r

Method used

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  • Stenotrophomonas acidaminiphila and application thereof
  • Stenotrophomonas acidaminiphila and application thereof
  • Stenotrophomonas acidaminiphila and application thereof

Examples

Experimental program
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Example Embodiment

[0034] Example 1

[0035] 1. Isolation of strains

[0036] (1) Collect the activated sludge from the sewage treatment plant and place it in a glass domestication device with a length of 31.2 cm, a width of 22.0 cm, and a height of 22.5 cm. The air compressor is not used on the basis of no nutrients and no exchange of influent and effluent. Intermittent aeration for 3 days. Then the water is changed, about 10L of mud-water mixture is discharged from the device, nutrient solution is added and continuous aeration is performed. After 10 days of cultivation, start adding pigment red 23 solution while adding nutrient solution, keep the concentration of pigment red 23 in the device at about 10 mg / L, and follow the SBR process (water intake, aeration, standing, draining, idle) for domestication Cultivate, change the water every other day, cultivate and acclimate for 30 days. Preparation of culture solution: 400mg / L glucose, 80mg / L anhydrous sodium acetate, 125mg / L NaHCO 3 , 4.75mg / L KCl...

Example Embodiment

[0048] Example 2

[0049] The strain was inoculated into pigment red 23 decolorization medium, and its OD600 and absorbance at 579nm were measured every 2h. Using the uninoculated decolorization medium as a blank control, the growth curve and pigment decolorization curve of the bacteria were drawn to determine the best The decolorization time is 18h. Select the carbon source, nitrogen source, inoculation amount, temperature, pH, salinity, study the best conditions of a single factor, and judge the influence of each factor on the growth of the strain and the decolorization effect of the pigment. Each factor is set with a different influence gradient. When studying the influence of one of the factors, only different influence levels are set for this factor, and other factors remain unchanged. The latter factors are carried out on the premise of the optimal conditions of each factor obtained in the previous experiment. All experiments set up three parallel experiments.

[0050] The ...

Example Embodiment

[0070] Example 3

[0071] Take 0.75g of sodium alginate to 50mL of sterile water, prepare it into a gel solution after it is completely dissolved under heating, and then cool to 30°C. Under aseptic conditions, mix 3 mL of bacterial suspension with the colloidal solution evenly. Use a 1mL syringe to quickly drop the mixture into the constantly stirring FeCl 3 In the solution, form uniform and regular balls, soak the balls in FeCl 3 In the solution, place it in a refrigerator at 4°C for 24h. Wash the solidified and crosslinked pellets with distilled water twice to obtain immobilized microbial pellets, such as Picture 9 Shown.

[0072] The optimal nutrient source concentration is yeast powder, 1g yeast powder, 0.1g / L CaCl 2 , 0.5g / L MgSO 4 ·7H 2 O, 1g / LKH 2 PO 4 , 1g / L Na 2 HPO 4 , 100mg / L Pigment Red 23, 1L distilled water to configure decolorizing medium, pH=6.5. Under aseptic conditions, dispense 100 mL of decolorizing medium into 250 mL sterile Erlenmeyer flasks.

[0073] Take ...

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Abstract

The invention relates to stenotrophomonas acidaminiphila and application thereof. The preservation number of the stenotrophomonas acidaminiphila SP-3 is CGMCC No.13711, a nucleotide sequences is shown as SEQ ID NO.1. The stenotrophomonas acidaminiphila is used for decoloration and degradation of pigment red 23 wastewater. The azo dye wastewater degradation effect of the stenotrophomonas acidaminiphila is good, an application method is simple, the cost is low, and the stenotrophomonas acidaminiphila has a very good application prospect.

Description

technical field [0001] The invention belongs to the field of dye decolorization and degradation, and in particular relates to microacidophilic stenotrophomonas and its application. Background technique [0002] Dyes, as an organic compound, can be dissolved in water and other media to dye fibers into various stable colors. The world has an annual output of about 800,000 tons of dyes, of which China's annual output is about 150,000 tons, ranking among the top in the world. The discharge of dyes first causes changes in the chromaticity of the water body, even if there is a small amount of dyes, it will cause a large change in the chromaticity of the water body, which not only affects the transparency and solubility of the water body, but also poses a threat to the survival of aquatic organisms. The dyes used in the printing and dyeing industry can be divided into various types according to their chemical structures. Among them, azo dyes are widely used and the largest amount ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C02F3/34C12R1/01
CPCC02F3/34C02F2101/308C12N11/10C12N1/205C12R2001/02
Inventor 赵晓祥孙璐璐周月白恒程晨
Owner DONGHUA UNIV
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