An aptamer-based method for the detection of mycotoxins in gold@dtnb@silver nanotriangles

A technology for mycotoxins and detection methods, applied in the fields of nanotechnology, nanotechnology, nanotechnology, etc. for materials and surface science, can solve the problems of poor signal stability, low detection sensitivity, single type of substrate material, etc., and achieve improved stability. Sexual and repetitive effects

Active Publication Date: 2019-08-27
JIANGSU UNIV
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  • Claims
  • Application Information

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Problems solved by technology

Surface-enhanced Raman spectroscopy has been applied to the detection of trace substances such as pesticides, antibiotics, and bacteria, but there are relatively few studies on the detection of mycotoxins, and the type of substrate material is single, with low detection sensitivity, poor signal stability, and detection limits. need further improvement

Method used

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  • An aptamer-based method for the detection of mycotoxins in gold@dtnb@silver nanotriangles
  • An aptamer-based method for the detection of mycotoxins in gold@dtnb@silver nanotriangles

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Embodiment 1

[0029] In order to further verify the sensitivity of the detection method prepared by the present invention to the detection of mycotoxins in food, examples of the present invention, taking aflatoxin B1 as an example, the specific operation steps are as follows:

[0030] Step 1) Preparation of gold nanotriangles: a three-step gold seed induction method is used to synthesize gold nanotriangles, specifically:

[0031] Gold Seed Solution 1:

[0032] 25 μL, 0.05M HAuCl 4 The solution was added to 4.7 mL of 0.1 M CTAC solution, and 300 μL of freshly prepared 0.01 M NaBH was added under vigorous stirring. 4 solution, put it at room temperature for 3h to remove excess NaBH 4 ;

[0033] Gold seed solution 2: 1.6 mL, 0.1 M CTAC solution was diluted with 8 mL of ultrapure water, and 40 μL, 0.05 M HAuCl was added 4 Solution, quickly add 0.01M, 15μL sodium iodide solution;

[0034] Growth solution: 1 mL, 0.05M HAuCl 4 Add the solution to 80mL, 0.05M CTAC solution, and quickly add 0....

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Abstract

The invention provides a detection method for mycotoxin based on an aptamer-modified gold@DTNB@silver nanotriangle. According to the method, a three-step seed induction method is employed for preparation of a gold nanotriangle, the gold nanotriangle is labeled with a 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) signal molecule and coated with a silver shell so as to synthesize a gold@DTNB@silver nanotriangle, and the gold@DTNB@silver nanotriangle is modified with a mycotoxin aptamer chain; a superparamagetic chitosan ferriferrous oxide (CS-Fe3O4) magnetic material is prepared and modified with a mycotoxin aptamer chain; and a detection system is constructed and a standard curve is established to implement detection. A DTNB molecular marker is prepared on the basis of the strong Raman enhancement effect of the gold nanotriangle, the silver shell is employed for protection, and a gold@DTNB@silver nanotriangle enhanced substrate with dual Raman enhancement effects is utilized; and quantitative ultra-sensitive detection of mycotoxin in food is realized via the separation effect of CS-Fe3O4, the surface-enhanced Raman effect of the gold@DTNB@silver nanotriangle and the specific recognition effect of the aptamers. The method is applicable to the technical fields of food safety, material chemistry and the like.

Description

technical field [0001] The present invention relates to the technical fields of food safety, material chemistry, etc., and specifically relates to an aptamer-based gold@DTNB@silver nano-triangle mycotoxin detection method. Background technique [0002] Mycotoxins are secondary metabolites produced by fungal growth. Peanuts and other grain foods are particularly susceptible to aflatoxin contamination due to improper storage and processing (including drying, processing, and storage), and have the potential to cause liver cancer, gastric cancer, and kidney cancer. At present, the conventional methods for mycotoxin detection mainly contain biological identification method, but this method can only be used for qualitative detection, the specificity is not strong, and the sensitivity is low; chemical analysis method (thin layer chromatography), its precision Poor, difficult to apply in practice; instrumental methods (gas chromatography, liquid chromatography, GC-MS, liquid-mass s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65B82Y30/00B82Y40/00B82Y5/00
Inventor 陈全胜杨明秀李欢欢欧阳琴郭志明孙浩刘妍
Owner JIANGSU UNIV
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