Bacillus subtillis HMB28948 and application thereof
A Bacillus subtilis and bacterial strain technology, applied in the field of biological control, can solve the problems of affecting the normal growth of cotton, poor chemical control effect, and the death of cotton seedlings in pieces, and achieves strong drug effect persistence, broad antibacterial spectrum and low cost. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] The preparation of embodiment 1HMB28948 microbial bacterial agent
[0051] Follow the steps below:
[0052] (1) Strain activation: the Bacillus subtilis strain HMB28948 stored at -80°C (this strain has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on October 28, 2016, and the preservation number is CGMCCNo.13212) Activate (30°C) on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), pick a single colony on LB Culture on slant medium (its composition and weight ratio is: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) at 30°C for 12 hours to obtain an activated strain;
[0053] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000mL), and put it in a...
Embodiment 2
[0056] Embodiment 2 Antagonism test of HMB28948 bacterial strain of the present invention to R. solani of cotton
[0057] (1) Source of R. solanacearum tested: R. solanacearum RH-2 strain was collected from a cotton blight disease strain in Qiu County, Handan City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Plant Pathology Department of the Conservation College identified it as Rhizoctonia solani (Rhizoctonia solani), and the pathogenicity test showed strong pathogenicity.
[0058] (2) Test method
[0059] Plate determination test: first activate and cultivate R. solani RH-2 on a PDA plate for 3 days, then use a puncher with a diameter of 6mm to punch holes in the edge area of the colony to make a bacterial sheet, and then place the bacterial sheet of R. solani Transfer to another PDA plate center, then the bacillus subtilis HMB28948 activated in the step (1) of embodiment 1 is s...
Embodiment 3
[0064] Embodiment 3 HMB28948 bacterial strain of the present invention is to the comparative test of control effect of cotton blight
[0065] (1) Test treatment:
[0066] (1) HMB28948 microbial agent: the HMB28948 liquid preparation prepared in Example 1 was diluted 50 times with water.
[0067] (2) Blank control: clear water
[0068] (2) Test method:
[0069] First, mix the vermiculite and cotton field soil according to the ratio of 1:1, sterilize under high pressure at 121 ℃ for 1 hour, and then sterilize again under the same conditions the next day. Inoculate the suspension of Rhizoctonia solani mycelium in 500 grams of soil (about 10 7 per milliliter) 10 milliliters, stir evenly, fill in a plastic flower pot with a diameter of 20 cm, and compact it. The cotton variety was Jifeng 106, and the seeds were soaked in the 50-fold water dilution of the HMB28948 liquid preparation prepared in Example 1 for half an hour before sowing; the seeds were soaked in clear water for ha...
PUM

Abstract
Description
Claims
Application Information

- Generate Ideas
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com