Bacillus subtillis HMB28948 and application thereof

A Bacillus subtilis and bacterial strain technology, applied in the field of biological control, can solve the problems of affecting the normal growth of cotton, poor chemical control effect, and the death of cotton seedlings in pieces, and achieves strong drug effect persistence, broad antibacterial spectrum and low cost. Effect

Active Publication Date: 2017-08-15
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cotton blight seriously affects the normal growth of cotton, and often leads to the death of cotton seedlings after the onset, which is an urgent problem to be solved
[0003] The control of cotton blight is mainly based on chemical control, including dressing, coating o

Method used

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  • Bacillus subtillis HMB28948 and application thereof
  • Bacillus subtillis HMB28948 and application thereof
  • Bacillus subtillis HMB28948 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Example 1 Preparation of HMB28948 microbial agent

[0051] Follow the steps below:

[0052] (1) Strain activation: Bacillus subtilis strain HMB28948 stored at -80°C (this strain has been deposited in the General Microbiology Center of the China Microbial Culture Collection and Management Committee on October 28, 2016, and the deposit number is CGMCCNo.13212) Activated (30℃) on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), pick a single colony on LB Inclined medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) cultured at 30°C for 12 hours to obtain activated strains;

[0053] (2) Preparation of seed solution: Prepare LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000mL), and fill it in a 250mL Erlenmeyer flask...

Example Embodiment

[0056] Example 2 The antagonistic effect test of the HMB28948 strain of the present invention on Rhizoctonia solani

[0057] (1) The source of the tested cotton Rhizoctonia solani: The cotton Rhizoctonia solani RH-2 strain was collected from the cotton wilt disease strain in Qiuxian County, Handan City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Plants of Hebei Agricultural University The Department of Plant Pathology of Conservation College identified Rhizoctonia solani as strong pathogenicity.

[0058] (2) Test method

[0059] Plate determination test: First, R. solanacearum RH-2 was activated and cultured on a PDA plate for 3 days, and then a hole punch with a diameter of 6 mm was used to make a plate at the edge of the colony, and then the R. solanacearum plate was made. Transfer to the center of another PDA plate, and then spot the activated Bacillus subtilis HMB28948 in step (1) of Exa...

Example Embodiment

[0064] Example 3 Comparative test of the control effect of the HMB28948 strain of the present invention on cotton blight

[0065] (1) Experimental treatment:

[0066] (1) HMB28948 microbial agent: The HMB28948 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0067] (2) Blank control: clear water

[0068] (2) Test method:

[0069] First, mix the vermiculite and cotton field soil in a ratio of 1:1, sterilize with high pressure at 121°C for 1 hour, and sterilize again with the same conditions the next day. Inoculate a suspension of Rhizoctonia solani hyphae (approximately 10 7 Pieces / ml) 10ml, mix well and fill it in a plastic flowerpot with a diameter of 20 cm, and compact it. The cotton variety was Jifeng 106, and the HMB28948 liquid formulation prepared in Example 1 was used for soaking seeds for half an hour before soaking in 50-fold water dilution; the seeds were soaked in clear water for half an hour as a blank control. After soaking, 10 seeds were sown ...

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Abstract

The invention discloses a bacillus subtillis HMB28948 strain. The strain is collected in CGMCC, and the collection number is CGMCC No.13212. The invention also discloses a microbial inoculant produced by means of the strain. The bacillus subtillis HMB28948 strain disclosed by the invention is high in efficacy on cotton damping-off, and the average control effect is 79.0% above. Secondly, drug resistance is unlikely to generate by using the strain to prevent and treat the cotton damping-off, and the efficacy durability is strong; in addition, the bacillus subtillis HMB28948 strain is wide in antibacterial spectrum. Besides the cotton damping-off, the bacillus subtillis HMB28948 strain also has very good inhibiting action on verticillium wilt of cotton, cotton-wilt fusarium, cucumber target spot or botrytis cinerea.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus subtilis HMB28948, a microbial agent produced by using the bacillus subtilis, and also relates to their use in preventing and treating plant diseases such as cotton blight. Background technique [0002] Cotton blight is a cotton seedling disease caused by Rhizoctonia solani Kühn. Its main symptoms are: cotton seeds are infected before germination and cause rotten seeds, and cotton seeds are germinated before they emerge. Infection causes rotten buds; after the cotton seedlings are unearthed, they are damaged, and at the initial stage, yellow-brown lesions are formed near the base of the soil surface, and the lesions gradually expand and surround the entire base, showing obvious constriction, and the diseased seedlings wilt, fall and die; when the diseased seedlings are pulled up, the stem base The lower cortex remains in the soil, and only the sharp rattai...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 郭庆港马平李社增鹿秀云张晓云王莹王培培赵卫松张丽红
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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