A strain of Pseudomonas aeruginosa xcs007 and its application in the control of tobacco black shank

A technology of Pseudomonas aeruginosa and thallus, applied in application, bacteria, fungicides and other directions, can solve the problems of poor effect of tobacco black shank, rarely produce chitinase, low activity, etc., and achieve biocontrol effect Good, not easy to produce drug resistance, environmentally friendly effect

Active Publication Date: 2020-01-17
中国烟草总公司海南省公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, although there are related researches on biocontrol bacteria, their effect on controlling tobacco black shank is poor, and chitinase is rarely produced, and even if chitinase is produced, its activity is low

Method used

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  • A strain of Pseudomonas aeruginosa xcs007 and its application in the control of tobacco black shank
  • A strain of Pseudomonas aeruginosa xcs007 and its application in the control of tobacco black shank
  • A strain of Pseudomonas aeruginosa xcs007 and its application in the control of tobacco black shank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Screening of Pseudomonas aeruginosa XCS007 and its antagonism to blackleg bacteria

[0051] 1.1 Isolation and screening of antagonistic bacteria

[0052] Soil samples were collected from tobacco fields with black shank diseased plants in Zhucheng Tobacco Experimental Station, Shandong Province, typical diseased plants and adjacent healthy plants were selected as objects, and rhizosphere soil at a depth of 5-10 cm was taken in order to obtain the anti-tobacco black Antagonist strains with highly effective antagonistic activity against Bacteria chinensis. Weigh 1g of diseased soil into a 10mL test tube with a stopper, add 9mL of sterilized water, shake at room temperature at 200r / min for 1h. Take 1 mL of the supernatant and dilute to 10 -4 、10 -5 、10 -6 times, spread evenly on the NA medium with a spreader, and place in a 37°C incubator for constant temperature cultivation for 3 days. Pick 21 strains of single colony with different growth characteristics, a...

Embodiment 2

[0075] Example 2 Pot experiment of Pseudomonas aeruginosa XCS007 preventing and treating tobacco black shank

[0076] Cultivation of black shank fungus: cultured with boiled rice grains. After culturing at 28°C for 5 days, black shank fungus hyphae grew all over the rice grains, and black shank fungi for infection were obtained.

[0077] Cultivation of Pseudomonas aeruginosa XCS007: in NB medium, 28°C, 120r / min, shaking culture for 48h, to obtain XCS007 bacterial liquid (for subsequent disease resistance treatment), the determination of bacterial liquid contains about 2.8-5.3 ×10 8 bacteria. Take 200mL of bacterial liquid, add ethyl acetate solution to the bacterial liquid according to the ratio of ethyl acetate: bacterial liquid = 2:1 for extraction, mix well and put it into a digitally controlled ultrasonic cleaner for ultrasonication for 30min with an ultrasonic energy of 90( Power), once every 8 hours, a total of 3 ultrasounds. After the ultrasonic vibration is complete...

Embodiment 3

[0095] Embodiment 3 field test of Pseudomonas aeruginosa XCS007 preventing and treating tobacco black shank

[0096] From May to August 2016, the purified and cultured XCS007 antagonistic bacteria solution and its crude extract were applied to two experimental fields in Kaiyang County and Xiuwen County, Guiyang City, where tobacco black shank disease occurred all year round. The root irrigation amount of each plant is the same as the pot test of embodiment 2. Spray or irrigate the roots 7 days, 14 days, and 21 days after transplanting the tobacco seedlings, and apply the pesticide 3 times in total, 3 repetitions, 15 plots, 100m per plot 2 , a total of 1500m 2 . In the early stage of tobacco topping, 30 representative tobacco plants were randomly selected in each plot for disease statistics, and the incidence rate (%), disease index and relative control effect (%) were calculated.

[0097] The incidence rates of tobacco plants treated with XCS007 bacterial liquid and its cru...

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Abstract

A strain of Pseudomonas aeruginosa XCS007, named Pseudomonas aeruginosa, has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation date is April 5, 2016, and the address is No. 1 Beichen West Road, Chaoyang District, Beijing Court No. 3, the deposit number is CGMCC No.12330. Pseudomonas aeruginosa XCS007 can efficiently produce chitinase, the highest activity reaches 12.25U / mL, and has excellent antagonism against tobacco black shank: the inhibition rate of bacterial liquid and crude extract of bacterial liquid on tobacco black shank mycelium Reach over 91%. Good biocontrol effect: For tobacco plants inoculated with tobacco black shank bacteria, the control effect of the bacterial liquid and the crude extract of the bacterial liquid can reach more than 80%. The use of biological control is friendly to the environment, not easy to produce drug resistance, safe for humans and animals, and creates conditions for the development of green tobacco control.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a strain of Pseudomonas aeruginosa XCS007 and its application in preventing and treating tobacco black shank. Background technique [0002] Tobacco black shank is an important fungal soil-borne disease caused by Phytophthora parasitica var. nicotianae. Tobacco black shank can infect and harm tobacco at any growth stage and existing cultivars. The average incidence rate is as high as 10%. The prevention and treatment of tobacco black shank usually adopts measures such as selection of disease-resistant varieties, chemical control, biological control, and strengthening of cultivation management. However, the abuse of chemical pesticides can easily induce resistance of pathogenic bacteria, and tobacco disease-resistant varieties are resistant to disease with the increase of service life. Problems such as gradual loss of sex are prominent. Biological control h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/385
CPCA01N63/00C12N1/205C12R2001/385
Inventor 陈德鑫林北森夏长剑刁朝强徐同伟胡勇杨艺炜祖庆学林松吴永琴李余江
Owner 中国烟草总公司海南省公司
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