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A high-sensitivity multi-target detection chromatographic test paper strip

A high-sensitivity, test strip technology, applied in the field of rapid, high-sensitivity, multi-target detection, can solve the problems of low sensitivity and specificity of immunochromatographic test strips, difficult to meet detection requirements, low efficiency, etc., and achieve enhanced Raman intensity. , cost saving, and the effect of improving detection efficiency

Pending Publication Date: 2017-08-29
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its advantages of ease of use, quickness, stability, and low cost, there are already a variety of commercial applications; the immunochromatographic test strips developed based on this have been widely used in clinical diagnosis, drug testing, inspection and quarantine, etc., with excellent performance , the market demand is huge; however, the sensitivity and specificity of immunochromatographic test strips are low, and it is difficult to meet the actual detection needs, and one test strip can only detect one substance, which has low efficiency and high cost

Method used

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  • A high-sensitivity multi-target detection chromatographic test paper strip
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  • A high-sensitivity multi-target detection chromatographic test paper strip

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: Chromatographic test strip for multi-target detection of methamphetamine (ice), cocaine, and morphine

[0045] 1. In this example, build the figure 1 The test strip shown is a chromatographic test strip for multi-target detection of methamphetamine (ice), cocaine and morphine based on Raman label coding. The test strip consists of a glass fiber sample pad (25mm*2mm), a glass fiber conjugate pad (5mm*2mm), a one-dimensional ordered cellulose acetate chromatography strip (20mm*2mm, the ordered structure period is 200nm) and an absorption Pad (25mm*2mm) according to figure 1 and 4 The sequence is pasted on the liner (66mm*2mm), and the overlapping length of the upper and lower overlapping parts of the edges of each part in the chromatography test strip is 3mm, and the glass fiber conjugate pad is adsorbed with movable colloid Gold (particle size 15nm)-Nile blue A labeled methamphetamine antibody, colloidal gold (particle size 15nm)-methylene blue labeled c...

Embodiment 2

[0048] Example 2: Chromatographic test strips for multi-target detection of neomycin, chloramphenicol, streptomycin and penicillin.

[0049] 1. In this example, a test strip as shown in FIG. 6(A) was constructed, which is a multi-target detection chromatographic test strip for neomycin, chloramphenicol, streptomycin and penicillin encoded by Raman tags. The test strip consists of a nylon membrane sample pad (26mm*4mm), a polyvinyl acetate conjugate pad (8mm*4mm), a two-dimensional silicon photonic crystal chromatography strip (20mm*4mm, the order structure period is 1500nm) and filter paper absorption The pad (28mm*4mm) is pasted on the pad (76mm*4mm) in turn, and the overlapping length of the upper and lower overlapping parts of the edges of each part in the chromatography test strip is 2mm, and the polyvinyl acetate conjugate pad is Adsorbed with mobile colloidal silver (particle size 40nm)-malachite green isothiocyanate-labeled neomycin antibody, colloidal silver (particle ...

Embodiment 3

[0052] Example 3: Clenbuterol hydrochloride, ractopamine, salbutamol, terbutaline and cimaterol multi-target detection chromatography test strips.

[0053] 1. In this example, a test strip as shown in Figure 8(A) was constructed, which coded Clenbuterol hydrochloride, ractopamine, salbutamol, terbutaline and cimaterol multi-targets based on Raman tags Test chromatography test strips. The test strip consists of a cellulose membrane sample pad (30mm*5mm), a non-woven conjugate pad (10mm*5mm), an inverse opal structure nitrocellulose chromatography strip (25mm*5mm, the ordered structure period is 3000nm) and The sponge absorbent pad (30mm*5mm) is pasted on the pad (92mm*5mm) in turn, and the overlapping length of the upper and lower overlapping parts of the edges of each part in the chromatography test strip is 1mm. The glass fiber conjugate Movable colloidal gold (particle size 60nm)-Nile blue A-labeled clenbuterol hydrochloride antibody, colloidal gold (particle size 60nm)-met...

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Abstract

A high-sensitivity multi-target detection chromatographic test paper strip is provided and used for detecting targets in a sample. A liner (1) is adopted as a substrate of the chromatographic test paper strip. A sample pad (2), a conjugate pad (3), a chromatographic strip (4) and an absorption pad (5) are disposed on the substrate in order from left to right. Metal nanometer particles (6) are fixed on the conjugate pad (3). The chromatographic strip (4) is provided with a detection line (7) and a quality control line (8). An edge of the sample pad (2) overlaps an edge of the conjugate pad (3). An edge of the conjugate pad (3) overlaps an edge of the chromatographic strip (4). An edge of the chromatographic strip (4) overlaps an edge of the absorption pad (5). The chromatographic test paper strip is used for measuring lateral flow, and achieves multi-target detection by utilizing a Raman spectrometer. The test paper strip can perform high-sensitivity and high-specificity multi-target detection and the detection cost is low. The test paper strip can be widely applied in the fields of inspection and quarantine, quality monitoring, environment protection, and the like.

Description

technical field [0001] The invention relates to a high-sensitivity multi-target detection chromatography test strip, which belongs to the field of rapid, high-sensitivity and multi-target detection. Background technique [0002] Lateral flow immunoassay (LFA) is a diagnostic method that emerged at the end of the 20th century. Due to its advantages of ease of use, quickness, stability, and low cost, there are already a variety of commercial applications; the immunochromatographic test strips developed based on this have been widely used in clinical diagnosis, drug testing, inspection and quarantine, etc., with excellent performance , The market demand is huge; however, the sensitivity and specificity of immunochromatographic test strips are low, and it is difficult to meet the actual detection needs, and one test strip can only detect one substance, which has low efficiency and high cost. [0003] Surface-enhanced Raman scattering (SERS) measures the amplified Raman scatteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N33/53
CPCG01N21/658G01N33/53
Inventor 赵祥伟张迪刘兵顾忠泽
Owner SOUTHEAST UNIV
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