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Microorganism for producing chitosanase, and plant cultivation composition and cultivation method using same

A technology for chitosan and decomposed products, which is applied to microorganisms producing chitosan decomposing enzymes and plant cultivation compositions and cultivation fields using the same, can solve the problem of difficulty in finding products, insignificant functional effects, and inconvenience to consumers. Trust and other issues, to achieve the effect of increasing the electron-donating capacity and increasing the content of polyphenols and flavonoids

Inactive Publication Date: 2017-09-05
株式会社可伶格林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, in order to produce environmentally friendly products, low-pesticide, no-pesticide, and environmentally-friendly farming methods have become essential elements, but in fact, it is almost impossible to maintain a commercial level in the production of agricultural products that do not use pesticides and fertilizers. impossible
[0007] Therefore, as a link in the production of eco-friendly agricultural products, research on eco-farming methods using microorganisms has been actively carried out. Along with many documents, various microorganisms and products related to microorganisms have been marketed, but most of them are produced by mixing various microorganisms. The form of effective microorganisms (EM bacteria, Effective Microorganisms) prepared has not been formally verified, so in fact, it is difficult to find products that can expect significant efficacy
[0008] In addition, in the case of functional agricultural products, when the active ingredient is artificially applied or cultivated, the production is carried out by methods such as soil treatment and irrigation, but the production has not been clearly verified for effectiveness, and the effect as a function is not significant multiple products, resulting in many cases of consumer distrust

Method used

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  • Microorganism for producing chitosanase, and plant cultivation composition and cultivation method using same
  • Microorganism for producing chitosanase, and plant cultivation composition and cultivation method using same
  • Microorganism for producing chitosanase, and plant cultivation composition and cultivation method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Selection of microorganisms

[0033] Prepare colloidal chitosan

[0034] Colloidal chitosan was prepared in the following manner: 400 mL of distilled water was added to 10 g of chitosan, and after stirring, 100 mL of 1 M acetic acid (acetic acid) was added thereto again, and after stirring for one night, filter paper was used. Filter, and after utilizing 1N sodium hydroxide (NaOH) to make filtrate neutralize, under the condition of 6000rpm, centrifuge 20 minutes, only collect precipitate, utilize distilled water to wash 3 times, and in sodium acetate buffer (sodium actetate buffer) (pH 5.5) so as to be suspended at 1%.

[0035] Isolation of strains

[0036] figure 1 It is a photo of the isolated chitosan decomposing enzyme producing strains C23 and C25 of the present invention.

[0037] In order to isolate chitosan-decomposing enzyme-producing microorganisms, 9 mL of sterilized distilled water was added to 1 g of soil collected in Gyeongbuk, Korea, and...

Embodiment 2

[0049] Example 2. Analysis of effect test by crop growth test - growth promotion

[0050] experimental method

[0051] The lettuce in this experiment uses under totally three kinds of conditions of no treatment group, control group (known microbial preparation sold on the market), C23 and C25 mixed treatment group (mass ratio is 1: 1), cultivated in pots. Lettuce, to investigate fertilizer damage and changes in crops.

[0052] At this time, in the mixed treatment group of C23 and C25, the two microorganisms were diluted 300 times with water for perfusion and foliar fertilization, and used instead of the existing water.

[0053] Fertility promotion experiment results

[0054] According to the survey results of lettuce growth, as shown in Table 1, in the non-treatment group and the control group, the leaf length is 11.6cm, but compared with this, in the treatment group, the leaf length is 12.4cm, which is 12.4cm. High, and in the treatment group, compared with the non-treatme...

Embodiment 3

[0058] Example 3. Analysis of Effect Test by Crop Growth Test - Total Content of Polyphenols

[0059] extraction conditions

[0060] Figure 5 and Figure 6 It is a photo of lettuce extract by ethanol extraction by cultivation time and by treatment group. The lettuce extract was prepared as follows: 1 g of cultivated lettuce was collected, mixed with 50 ml of 80% ethanol, left to extract at room temperature (25°C) for 24 hours, and centrifuged to recover the supernatant .

[0061] Analysis method for the total content of polyphenols

[0062] The total content of polyphenols was determined as follows: according to the Folin-ciocal method, in the prepared 1ml analysis sample, add 1ml of Folin-ciocalteu reagent (SigmaChemical Co., St. .Louis, MO, USA) solution, and at room temperature, after standing for 5 minutes, add 4ml of 75% Na 2 CO 3 The solution was added for an additional reaction for 1 hour, and the absorbance was measured at 756 nm using a Microplate reader (Infi...

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PUM

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Abstract

The invention relates to a microorganism for producing chitosanase, and a plant cultivation composition and cultivation method using the same, in particular to a lettuce cultivation method based on Paenibacillus mucilaginosus C23 (collected under KACC91963P) and Bacillus ehimensis (collected under KACC91964P); compared with traditional chitosan-decomposing microorganisms, the microorganism used in the method provided herein provides decomposition at higher speed; when a chitosan fermentation using the microorganism applies to lettuce cultivation, the microorganism promotes the growth and development of lettuce and adds more functional ingredients and functions (polyphenols, flavonoids and electron donating capacity).

Description

technical field [0001] The present invention relates to Paenibacillus mucilaginosus C23 (Accession No.: KACC91963P), Paenibacillus ehimensis C25 (Accession No. : KACC91964P) microorganisms and plant cultivation compositions and cultivation methods using the same are lettuce cultivation methods that increase the content of polyphenols, flavonoids and electron donating ability compared with existing traditional cultivation. Background technique [0002] Due to the environmental changes caused by industrial development, it is difficult to manage the crops in their natural state, and a lot of pesticides are used for quick effects, so that beneficial bacteria can be eliminated along with harmful bacteria due to indiscriminate bactericidal power. [0003] Moreover, at the point in time when the bactericidal power of the pesticide disappears, the microorganisms start to reproduce again, and most of the pathogenic microorganisms reproduce first compared with the beneficial microorga...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07C05F11/00C05F11/08A01G1/00
CPCC05F11/00C05F11/08C12N1/205C12R2001/07
Inventor 李炫锡崔真荣崔元日郑熙暻郑留锡
Owner 株式会社可伶格林
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