70 results about "Bacillus ehimensis" patented technology

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

The invention provides a pathogen inspection reagent, and concretely discloses a primer probe combination and a kit for combined inspection of 15 kinds of respiratory tract infection pathogens. By aiming at specific target sequences of the gene sequence conserved region of klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, chlamydia pneumoniae, mycobacterium tuberculosis, stenotrophomonas maltophilia, baumanii, mycoplasma pneumoniae, enterococcus faecalis, ligionella pneumohpillia, streptococcus pneumoniae, bacillus pyocyaneus and mycobacterium abscessus, primers and probes which do not have mutual crossed reaction are designed, so that the problem that detection probes of different pathogens can easily generate mutual influenceor interference is solved; the combination and matching of different primers and probes are ensured; the goal of simultaneously performing efficient specific inspection at the same temperature can also be achieved.

The invention relates to the technical field of medical detection, and in particular, relates to a primer and probe combination and a kit for detecting human pathogenic bacteria. The kit comprises theprimer and probe combination for detecting pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, staphylococcus epidermidis, staphylococcus aureus, enterococcus faecium, human staphylococcus and acinetobacter baumannii. According to the kit, the primer and probe combination is adopted, multiple digital PCR is combined, the pathogenic bacteria types and the target nucleic acid copy number in the detection range can be rapidly and accurately identified, the detection method has high sensitivity, and positive detection of low-copy nucleic acid fragments of pathogenic bacteria is guaranteed; meanwhile, the variety of the pathogenic bacteria or the change of the copy number of the target nucleic acid fragment in the body of a pathogenic bacteria infected patient can be dynamically monitored, the treatment effect is assisted and evaluated in time, and reference is provided for optimization of a doctor clinical scheme.

The invention provides a spawn preservation method adopting semisolid low-temperature air-tight culture. A spawn is inoculated in an aseptic semisolid culture medium which is vertically stored at an ambient temperature of between 6 and 8 DEG C after aseptic liquid paraffin is added into the culture medium. The spawn is staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus subtilis, clostridium orogenes, aspergillus niger or candida albicans. The specific process is as follows: preparing the semisolid culture medium, carrying out sub-package and sterilization, and then culturing for 2 to 3 days, inoculating the spawn into the semisolid culture medium in a mode of stab inoculation after asepsis is ensured, with the number of stab inoculation points parallel with the plane of the culture medium being no less than 3; pouring the aseptic liquid paraffin with a height of 1 to 2 cm into the culture medium which is sealed by a sealing film and is stored in a refrigerator at a temperature of between 6 and 8 DEG C. The method can not only prolong the spawn preservation time but also keep a relatively stable spawn concentration, thereby saving much cost for production and test, reducing the treatment and discharge of rejected material after spawn use, and reducing environmental hazard.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

Disclosed are a preparation method and application of Anaphalis lactea essential oil. Volatile oil in Anaphalis lacteal is extracted by steam distillation and CO2 supercritical extraction, ingredients of the volatile oil are analyzed initially by gas chromatography / mass spectrography, 57 ingredients in the volatile oil are identified and account for more than 94% of the total volatile oil. Antibacterial and bactericidal activities of the volatile oil are researched by the Oxford cup method, and according to the research, the volatile oil has an evident killing or inhibiting function for bacteria, such as staphylococcus, streptococcus, enterobacter, salmonella, shigella, pseudomonas, bacillus, escherichia coli and the like, and fungi, such as candida, cryptococcus, penicillin, aspergillus, mucor, microsporum, trichophyton, epidermophyton and the like. Testing by DPPH (diphenylpicrylhydrazyl) method shows that the volatile oil has fine antioxidant activity. Owing to the fine activity, the volatile oil is widely applicable to the industries of foods, medicines, cosmetics, health-care products and the like.

The invention relates herba dracocephalum heterophylli volatile oil possessing the bacteriostatic and germicidal action and its application. The method adopts steam distillation to extract the herba dracocephalum heterophylli volatile oil and adopts gas-chromatography / mass spectra to analysis the volatile oil, identifying the 83 components and 89.83% total content. The volatile oil can kill and suppress the staphylococcus, streptococcus, gonococcus, enterobacter, Escherichia, salmonella, shigella, pseudomonas, bacillus, oidiomycin, cryptococcus, aspergillus, penicillium, mucor, epidermophytin, micromonospora, trichophyton and other bacteriums. The herba dracocephalum heterophylli volatile oil is used for foodstuffs, drugs, health products, cosmetic products and other industries.

The invention aims to provide a multiple PCR detection kit and a method for identifying susceptible bacteria species for intracranial post-operation to eliminate endogenous nucleic acid contamination. The detected bacteria species are the following common bacteria: staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, escherichia coli, bacillus pyocyaneus, klebsiella pneumoniae and acinetobacter baumannii. Meanwhile, the invention provides the method to eliminate the endogenous nucleic acid contamination in a raw material of a PCR system and exclude a false positive experimental result of a PCR caused by the endogenous nucleic acid contamination. The detecting method using the kit has the advantages of that the operation is simple and fast, the detection cost is lowered, the detection result is accurate, and the false positive result is reduced. A negative control and a positive control are added in the amplification step, so that the judgment of false positive and false negative is effectively improved, and the detection accuracy is higher.

The invention relates to application of chenopodium vulvaria volatile oil to bacteriostatic / bactericidal and antioxidative products. The chenopodium vulvaria volatile oil can be obtained by a water steam distillation or CO2 supercritical extraction method, and main ingredients of the chenopodium vulvaria volatile oil are identified by gas chromatograph / mass spectrum. The chenopodium vulvaria volatile oil has an obvious effect of killing or inhibiting bacteria such as staphylococcus, streptococcus, enterobacter, salmonella, shigella, pseudomonas, bacillus, escherichia coli and the like and fungi such as candida, cryptococcus, penicillin, aspergillus, mucor, microsporon, trichophyta, epidermophyton and the like. A diphenyl picryl phenylhydrazine (DPPH) method proves that the chenopodium vulvaria volatile oil has the high antioxidant activity, can be widely applied to industry of food, medicines, cosmetics, health-care products and the like, and is used for preparing the bacteriostatic / bactericidal and antioxidative products.

The invention relates to the field of microecological feed additives, and particularly relates to preparation and application of a composite microecological feed additive. The composite microecological feed additive provided by the invention is prepared from screened active compound probiotics. Diarrhea caused by domestic animal bacterial infections can be prevented and treated. Two probiotics used by the composite microecological feed additive have the abilities of inhibiting infection of common gastrointestinal pathogens of animals, including shigella dysenteriae, pathogenic escherichia coli and salmonella enteritidis, and also can reduce the toxicity caused by dysentery bacillus infection Caco-2 cells and inhibit adhesion of the shigella dysenteriae to the Caco-2 cells. Finally, the obvious effects of stimulating the animal immunity, improving the gastrointestinal digestion abilities of animals, and promoting animal growth are achieved. A production technology of a strain adopts a liquid fermental cultivation technology and a low-temperature freeze drying technology, so that the obtained inoculant is high in survival rate and purity. The composite microecological feed additive is a green environment-friendly feed additive.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

The invention relates to the technical field of microorganisms, in particular to streptococcus symcsis 3-17 streptococcus and an application thereof. Experiment proves that the streptococcus providedby the invention is named as streptococcus symcsis sp.nov.3-17, and is registered and preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC 60684; and the streptococcus has broad spectrum resistance and long-term antagonizing stability, and can resist one or more of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, proteus vulgaris, enterobacter cloacae and acinetobacter baumannii. The streptococcus GDMCC 6068 provided by the invention can be applied as probiotics, corresponding products capable of being practically applied can be prepared from the streptococcus GDMCC 6068, and the streptococcus GDMCC 6068 has quite application tremendous potential in the field of medicine and pharmacy.

The invention discloses a broad spectrum type antimicrobial peptide (AMP) and application thereof and belongs to the technical field of medicine. The AMP is named 26 M, contains 18 amino acid residues, has a molecular weight of 2475.16 Daltons, has an isoelectric point of 12.03, and has an amino acid sequence shown in SEQ ID NO: 1. Experiments show that the AMP has the effect of significantly inhibiting the growth and reproduction of various pathogenic bacteria including Escherichia coli, Salmonella, Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Streptococcus suis, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium. The invention provides the novel AMP with a broad-spectrum antimicrobial effect,and the AMP can replace traditional antibiotics and cannot produce drug resistance easily, and has good application prospects in resisting infectious diseases caused by pathogenic bacteria.

The invention discloses a traditional Chinese medicinal composition for treating edema disease of a bamboo rat. The traditional Chinese medicinal composition consists of the following raw medicinal materials in parts by weight: 2-4 parts of cordate houttuynia, 2-4 parts of blackberry lily, 2-4 parts of dandelion, 2-4 parts of sculellaria barbata, 2-4 parts of coptis chinensis and 2-4 parts of root of Chinese pulsatilla, and the composition is prepared by mixing or decocting the various traditional Chinese medicinal raw materials after respectively grinding. The traditional Chinese medicinal composition has the functions of clearing away heat and toxic materials, relieving swelling and pain, resisting inflammation, stopping blooding, removing dampness, killing parasites and the like; the composition is good in antibacterial performance, has a relatively good effect on inhibiting or killing escherichia coli and not easy to result in drug resistance, and is capable of enhancing immunity of the bamboo rat, reducing use of biotic, avoiding abuse of the biotic and benefiting for health of the bamboo rat. The traditional Chinese medicinal composition has relatively high curative rate and effective rate on treating edema disease of the bamboo rat, and is stable in effectiveness; the composition is abundant in crude medicine resource, appropriate in proportion and convenient to use and has an obvious treatment effect.

The invention discloses an expression purification method of a mycobacterium tuberculosis fusion protein LT29. An active-period antigen EAST6 of mycobacterium tuberculosis and an incubation-period antigen Rv2626c of the mycobacterium tuberculosis are fused and connected by a flexible peptide fragment so as to construct the fusion protein ESAT6-linker-Rv2626c. The fusion protein is subjected to expression purification in escherichia coli B21 engineering bacteria, and is combined with an adjuvant DP to construct a tuberculosis subunit vaccine. The fusion protein is successfully expressed and purified in escherichia coli; strong specific cellular immunoreaction aiming at the specific antigens (ESAT6 and Rv2626c) of the mycobacterium tuberculosis can be induced to be generated in a mouse body, the generation of long-term memory T cells is promoted, and the fusion protein has strong immunogenicity. According to the invention, the novel fusion protein LT29 without a label is successfully constructed, expressed and purified, the protein fuses the active-period antigen and the incubation-period antigen of tubercle bacillus, antigen-specific cellular immunity and humoral immunity can be induced to be generated, and the novel fusion protein LT29 is expected to become an effective candidate vaccine for preventing and treating tuberculosis.

The invention discloses a plasma free DNA marker for bloodstream infection pathogen diagnosis. The invention provides a plasma free DNA treatment method comprising the following step: selecting a fragment with a size of 60-140 bp from to-be-tested plasma free DNA. The method can be used as a sample pretreatment method for bloodstream infection pathogen detection through the plasma free DNA. The distribution range of free nucleic acid in plasma samples infected with Escherichia coli, Klebsiella pneumoniae, staphylococcus aureus, enterococcus faecium and Acinetobacter baumannii is studied, and the distribution of free nucleic acid fragments of the five bacteria in plasma is determined. The plasma free DNA marker has important significance of improving the sensitivity of pathogen detection inplasma metagenome detection and guiding the design of the length range of PCR amplification product fragments to improve the PCR detection sensitivity. The plasma free DNA marker provides a basis forpreparation of pathogen simulation samples in a plasma free DNA detection product development process.

The invention discloses a soil repairing complex microbial inoculant for degrading soil fertilizer and medicine residues and a soil repairing method of the soil repairing complex microbial inoculant. The soil repairing complex microbial inoculant for degrading soil fertilizer and medicine residues comprises the following components in parts by weight: 2-6 parts of Tsuruhada delftia acidovorans, 2-6 parts of escherichia hermannii, 2-6 parts of bacillus subtilis, 2-6 parts of streptomycete, 1-5 parts of beer yeast and lactobacillus plantarum, 1-5 parts of acinetobacter, 1-5 parts of bacillus licheniformis and 1-5 parts of gordon amarae.

The invention discloses a mesoporous bioactive glass / lasiosphaera composite material, preparation and applications, and belongs to the biomedical material field. The composite material is mainly composed of mesoporous bioactive glass and lasiosphaera. The preparation technology method is advantaged by simple preparation, controllable technological parameters, convenient operation and low cost. The obtained composite material has good hemostatic effects. In addition, the composite material has certain inhibition effects on staphylococcus aureus, pseudomonas aeruginosa, proteusbacillus vulgaris, diplococcus pneumonia, typhoid bacillus and escherichia coli, has inhibition effects on few pathogenic fungi, has good biological activity, and can be used as an integrated composite material of antibiosis, hemostasis and promoting tissue regeneration.

The application provides a primer and / or probe composition for detecting bacillus for initiating blood flow infection and an application of the primer and / or probe composition, and particularly relates to one or more primer and / or probe compositions or relevant reagents or reagent kits for detecting mycobacterium tuberculosis, listeria monocytogenes, klebsiella pneumoniae, pseudomonas aeruginosa,baumanii, escherichia coli, haemophilus influenzae and proteus mirabilis, so that the detection period of the bacillus can be greatly shortened, the efficiency and the detection flux are improved, andthe primer and / or probe composition can meet detection requirements. In addition, the method and relevant products thereof disclosed by the application have high specificity, sensitivity and accuracy.

The invention relates to a colorectal cancer biomarker as well as a screening method and application thereof, and belongs to the technical field of cancer diagnosis. The colorectal cancer biomarker provided by the invention comprises human intestinal microorganisms and / or fecal human target genes; the intestinal microorganisms of the human body comprise one or more than two of Brawtella, Pseudomonas 9, Fecal bacillus, fusiform, Wehonor coccus, Escherichia coli-Shigella, Mycobacterium, Fusobacterium, Porphyrinium, peptostreptococcus and Bacteroides; the fecal human body target spot gene comprises ADHFE1 and / or SDC2. The biomarker combination disclosed by the invention is high in sensitivity and good in specificity, and has a profound prospect for estimating the risk of colorectal cancer. According to the invention, a data model is established through the biomarker, and the model can be used for evaluating the risk of colorectal cancer.

The invention discloses a probe combination, and belongs to the technical field of microbiological detection. The probe combination comprises probes for detecting at least two pathogenic bacteria of escherichia coli, staphylococcus aureus, listeria monocytogenes and shigella sonnei. The invention also discloses a gene chip prepared by using the probe combination, a kit and a detection method. The method is used for detecting four food-borne pathogenic bacteria, is strong in specificity, high in sensitivity, good in accuracy, convenient and rapid, is particularly suitable for detecting samples with low bacterial concentration, and provides better technical support for infection diagnosis and treatment and food safety detection.

The invention relates to a microorganism for producing chitosanase, and a plant cultivation composition and cultivation method using the same, in particular to a lettuce cultivation method based on Paenibacillus mucilaginosus C23 (collected under KACC91963P) and Bacillus ehimensis (collected under KACC91964P); compared with traditional chitosan-decomposing microorganisms, the microorganism used in the method provided herein provides decomposition at higher speed; when a chitosan fermentation using the microorganism applies to lettuce cultivation, the microorganism promotes the growth and development of lettuce and adds more functional ingredients and functions (polyphenols, flavonoids and electron donating capacity).

The invention discloses a microbial limit inspection method for coptis chinensis formula granules. The microbial limit inspection method comprises the following steps: S1, respective preparation of bacterial suspensions of staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida albicans and aspergillus niger; S2, preparation of a test solution: preparing the test solution containing 3% histidine; S3, applicability test on a counting method for the total number of aerobic bacteria; S4, applicability test on a counting method for the total number of molds and saccharomycetes; and S5, applicability test on an Escherichia coli inspection method, wherein the limit detection standard is as follows: in 1 g of a test sample, the total number of aerobic bacteria is less than or equal to 103 cfu, the total number of molds and saccharomycetes is less than or equal to 102 cfu, and Escherichia coli cannot be detected. The method can be used for limit inspection of microorganisms in the coptis chinensis formula granules, and has the advantages of effectively eliminating the antibacterial activity of a sample, simplifying the inspection process, reducing the reagent dosage and efficiently detecting the microorganisms in the coptis chinensis formula granules.

A method of controlling the presence of bacteria that belong to the group of enterobacteriaceae, for example, Salmonella and / or Escherichia species, in a monogastric animal, by feeding the monogastric animal with a feed material that comprises mycelium of Agaricus Blazei Murill (ABM mycelium), e.g., grown on a grain substrate.

The invention relates to the field of biological detection, and particularly relates to a substrate combination, a detection reagent and a detection kit for detecting escherichia coli and / or shigella.The substrate combination provided by the invention is a detection kit for quickly distinguishing escherichia coli and the shigella based on biochemical and chromogenic principles and a preparation method thereof is provided. By using the kit disclosed by the invention, the escherichia coli, shigella bogdii, shigella sonnei, shigella flexneri and shigella dysenteriae can be quickly distinguishedwithin 1-2 hours by only one step of operation.

The invention relates to application of a Chinese medicine composition in preparation of a medicament for treating the overhigh level of a urine cell factor or a receptor thereof and Escherichia coli infection. The cell factor or receptor thereof is sIL-2R, IL-6 and IL-8. The Chinese medicine composition is prepared from the following raw materials in percentage by weight: 14-20 percent of cherokee rose root, 6-10 percent of chinaroot greenbrier, 7-10 percent of akebia trifoliata koidz, 3.0-6.5 percent of Japanese climbing fern and 3.2-6.8 percent of centella. The Chinese medicine composition has obvious curative effect on urinary tract infection with overhigh level of urinary sIL-2R, urinary IL-6 and urinary IL-8 and urinary tract infection and lower respiratory tract infection with Escherichia coli as a main pathogenic bacterium.

The invention belongs to the technical field of medicines. The invention relates to an antibacterial composition containing avibactam and meropenem and application thereof. Particularly, the inventionprovides application of avibactam or a pharmaceutically acceptable salt thereof and meropenem in preparation of a medicine for treating or preventing bacterial infection, in particular to applicationin preparation of medicines for treating infection caused by escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii and enterobacter aerogenes, and aims to solve theproblem of drug resistance of carbapenem antibiotics.

The invention discloses a traditional Chinese medicine composition and application thereof. The traditional Chinese medicine composition comprises 55-75 parts of radix lithospermi, 5-15 parts of flos carthami, 5-15 parts of galla chinensis, 5-10 parts of herba speranskiae tuberculatae, 4-10 parts of bletilla striata and 2-7 parts of cortex phellodendri. The traditional Chinese medicine composition can remarkably promote keratinocyte migration, removes DPPH free radicals, has an obvious antibacterial effect on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, and further has an effect of inhibiting NO, and it is indicated that the traditional Chinese medicine composition has potential application in the aspects of promoting wound healing, resisting oxidation, resisting bacteria, resisting inflammation, adjusting immunity and the like.

The invention relates to preenrichment fluid for vibrio vulnificus detection. A nitrogen source, a carbon source, inorganic salt, vitamins and other growth factors are added to the preenrichment fluid according to the biochemical features and culture features of vibrio vulnificus, and the preenrichment fluid has oxidation reduction potential, pH value and osmotic pressure suitable for the growth of the vibrio vulnificus. Compared with the alkaline peptone water widely used clinically and in food detection, the preenrichment fluid can facilitate the growth and resuscitation of the vibrio vulnificus in to-be-detected samples, can shorten culture time and increase positive detection rate, and can effectively inhibit growth and reproduction of other non-objected bacteria such as escherichia coli, pseudomonas and other gram positive bacteria. The preenrichment fluid is convenient to prepare, low in cost, high in enrichment ability and applicable to vibrio vulnificus detection of various levels of hospitals and detecting institutions.