12 results about "Bacillus ehimensis" patented technology

The invention relates to the technical field of functional food, and particularly discloses a preparation method and application of origanum vulgare essential oil oral nanoemulsion, the preparation method comprises the following steps: by taking origanum vulgare essential oil as an oil phase, PEG-40 hydrogenated castor oil as a surfactant and propylene glycol as a cosurfactant, determining the mass ratio of the surfactant to the cosurfactant to be 5: 1 through optimization, and preparing the origanum vulgare essential oil oral nanoemulsion. And determining the final prescription composition by using the quasi-ternary phase diagram. The oregano essential oil oral nano-emulsion provided by the invention is a faint yellow transparent clarified liquid, is good in stability, has a remarkable bacteriostatic effect on staphylococcus aureus and escherichia coli, solves the problems of poor water solubility, poor stability and strong oral irritation of the oregano essential oil, and provides a good basis for the application of the oregano essential oil as an oral preparation.

The invention relates to a flora prediction model for detecting premature delivery risk and a kit and application thereof. Through early-stage mNGS data collection and analysis, premature related strains are obtained, and through a large number of experimental screening and optimization, a set of optimal primer probe group is finally determined. The invention relates to a premature delivery risk calculation method, which comprises the following steps of: selecting 12 floras, namely lactobacillus crispatus, lactobacillus gasseri, lactobacillus inertus, lactobacillus jensenii, escherichia coli, enterococcus faecalis, enterobacter aerogenes, group B hemolytic streptococcus, ureaplasma parvum, chlamydia trachomatis, diplococcus gonorrhoeae and gardnerella vaginalis, and establishing a fluorescent quantitative PCR (Polymerase Chain Reaction) method and a premature delivery risk calculation model aiming at the detection of the 12 floras. Compared with an existing detection method, the method is simple, convenient, rapid, high in sensitivity and high in specificity, the false positive and false negative risk is reduced, and the purpose of batch detection is achieved. Clinically, the change of microbial flora in the reproductive system of a pregnant woman can be rapidly detected, corresponding treatment measures are taken, the risk of PTB occurrence is reduced, and the kit has a good application prospect.

This invention belongs to the field of microbial technology, specifically relating to the multifunctional Lactobacillus curvatureii and its application in the preparation of health products. The Lactobacillus curvatureii (… Lactobacillus crispatus Lactobacillus crispatus The accession number for MCOlact00102 is CGMCC No. 34381. The described *Lactobacillus curvatureensis* (MCOlact00102) is... Lactobacillus crispatus ​ The two strains, MCOlact00126 (CGMCC No. 34382), exhibit significant inhibitory effects against bacterial vaginosis (BV) and infections caused by Gardnerella vaginalis, Group B Streptococcus (GBS), Escherichia coli, or Candida albicans. The safety of these strains was evaluated using a 3D vaginal epithelial organoid model, showing no irritant effect on cells. Furthermore, hemolytic activity tests, antibiotic resistance gene detection, and functional validation all demonstrated their good safety profile. In addition, the aerobic acclimation process significantly improved the stability and bioactivity of these two strains in actual production and clinical applications, laying a solid foundation for their industrialization and application.

The application discloses a solubilization-stable liquid preparation of Trollius chinensis Bunge and a preparation method thereof. Anionic surfactant is used as a micelle carrier. Through a stable micelle structure formed in the water phase, the poorly soluble active ingredients in Trollius chinensis Bunge extract are dissolved and uniformly dispersed in the water phase, so that the solubility and system stability are significantly improved, and the bacteriostatic effect is improved. Through screening of formula components and optimization and adjustment of the composition ratio, the application successfully prepares a clear liquid preparation with excellent bacteriostatic activity from the poorly soluble substances of Trollius chinensis Bunge. The inhibition rate of the preparation on Escherichia coli, Staphylococcus aureus and Candida albicans is more than 90% after 30s of contact. The preparation has good stability after being placed at room temperature for 24 months, and the inhibition rate on the three test bacteria is still more than 80%. The concentration of Trollius chinensis Bunge extract required to achieve the same or even better bacteriostatic effect is far lower than 1 / 16 of the Trollius chinensis Bunge liquid preparation assisted by dimethyl sulfoxide (DMSO) organic solvent.

This invention discloses an antimicrobial 10-peptide, characterized by the sequence: w-k-r-w-r-r-w-w-r-r(dTrp-dLys-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dArg), where all amino acids are D-type amino acids. The antimicrobial peptide provided by this invention exhibits good antimicrobial activity against a variety of pathogenic bacteria. This antimicrobial peptide can be synthesized using the Fmoc solid-phase chemical method, which is easy to synthesize and exhibits good in vivo antimicrobial activity. This antimicrobial peptide exhibits broad-spectrum killing activity against multidrug-resistant Acinetobacter baumannii, carbapenem-resistant Pseudomonas aeruginosa, as well as standard strains of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, methicillin-resistant Staphylococcus aureus, and Staphylococcus aureus. It has low hemolytic toxicity, and at low to medium concentrations (2 μg / mL to 32 μg / mL), the cell survival rate can reach more than 50%. It has no toxic effect on cells, good stability, strong operability, and low cost.

The invention relates to the technical field of biology, in particular to a rapid detection kit for bacterial food-borne pathogenic microorganisms and application of the rapid detection kit. The invention develops a primer probe combination kit for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens through optimization of primers, optimization of hydrogel components and a system and the like. The primer probe combination kit is used for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens. The kit is used for detecting salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, Escherichia coli O157 and clostridium perfringens. The kit is high in sensitivity and specificity, strong in anti-interference capability, excellent in stability and portability, rapid and efficient in detection, simple and convenient to operate and low in cost, and can realize result visualization and relative quantification, so that the kit has wide application prospects and values.

The invention discloses a lactobacillus reuteri and a preparation method and application of a microbial inoculum of the lactobacillus reuteri, the lactobacillus reuteri subsp.reuteri OH114 is preserved in Guangdong Province Microbial Culture Collection Center (GDMCC), the preservation address is No.59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou, the preservation number is GDMCC No.66528, and the preservation time is June 16, 2025. The lactobacillus reuteri newly researched and developed by the invention can effectively inhibit the growth of a plurality of enteropathogenic bacteria such as staphylococcus aureus, escherichia coli, salmonella typhimurium, clostridium difficile, clostridium perfringens, klebsiella pneumoniae, pseudomonas aeruginosa and candida albicans, regulate the balance of intestinal flora, prevent and treat adult diarrhea, and prevent and treat diarrhea. The invention provides a new scheme and way for preventing and treating infectious diarrhea, antibiotic-related diarrhea or irritable bowel syndrome diarrhea.

The present application relates to the technical field of conjugative transfer engineering bacteria, and relates to a conjugative transfer donor bacterium, a construction method and application thereof, the conjugative transfer donor bacterium is Escherichia fergusonii EFmg-sy, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2025310;Or, the conjugative transfer donor bacterium is Escherichia fergusonii EFmg-cj, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M20251730.The conjugative transfer donor bacterium provided by the present application is stable in property, simple in operation as a conjugative transfer donor, can be quickly detected and distinguished from the receptor bacterium that has received plasmid through the method of fluorescence detection, and the detection of the plasmid horizontal transfer ability of Escherichia fergusonii is realized.

This invention discloses a method for preparing a strain of Lactobacillus saccharidus and its application in the preservation of chilled fresh meat, including the following steps: Step (1) Initial screening: Take 1.0 g of environmental sample, add 9 mL of sterile physiological saline, shake at 37℃ and 200 r / min for 30 min, sonicate for 10 min, then serially dilute to 10⁻⁴-10⁻⁶, take 100 μL and spread it on MRS solid medium, incubate at 37℃ for 48 h, pick single colonies and purify 3 times; Step (2) Antibacterial screening: Use 8 strains of putrefactive bacteria such as Enterobacter and Escherichia coli as indicator bacteria. The preparation method of this Lactobacillus saccharidus strain is based on natural sources, ensuring high safety and making it a suitable alternative to chemical preservatives. It exhibits significant inhibitory effects on major spoilage bacteria (such as Pseudomonas and Enterobacteriaceae) and pathogenic bacteria in chilled meat. Its low-temperature resistance (4℃) and acid and alkali resistance make it suitable for the storage environment of chilled meat. After application, it can reduce the total bacterial count, TVB-N content, pH value, and drip loss rate, delay color changes, and effectively extend the shelf life of chilled meat.

The present application relates to the technical field of microfluidics, and particularly relates to a kit for rapidly detecting 15 respiratory pathogens and a use method thereof.The kit comprises a plurality of reaction cavities, each of which has a primer group corresponding to 15 respiratory pathogens; the primer group comprises inner primers, outer primers and loop primers for PCR amplification of Mycoplasma pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Haemophilus influenzae, Streptococcus pneumoniae, Escherichia coli, Legionella pneumophila, Burkholderia, Staphylococcus aureus, Corynebacterium striatum, Aspergillus, Pneumocystis jirovecii and Candida albicans.The present application can simultaneously detect 15 respiratory pathogens by adding samples once, avoids adding liquid reagents to multiple detection tubes, reduces the intensity of manual operation and the risk of mismatch.

The application provides a chaetomium globosum, a method for separating secondary metabolites of the chaetomium globosum and application, and belongs to the technical field of biological medicines. Arcopilus cupreus DGR-7 has been preserved in Guangdong Microbial Culture Collection Center on October 10, 2025, with a preservation number of GDMCC No: 67068; the application first utilizes rice medium to ferment A. cupreus strains, and 8 compounds are separated and purified from the strains, wherein 6 compounds are anthraquinones. In-vitro antibacterial experiments show that the chaetomium globosum Arcopilus cupreus The secondary metabolites of DGR-7, anthraquinones, have good bioactivity in inhibiting the growth of staphylococcus aureus, methicillin-resistant staphylococcus aureus, streptococcus salivarius, klebsiella pneumoniae, bauman acinetobacter, pseudomonas aeruginosa and escherichia coli.

The invention provides chaetomium hordeum, a method for separating secondary metabolites from chaetomium hordeum and application of the chaetomium hordeum, and belongs to the technical field of biological medicine. The chaetomium cupreum DGR-7 is preserved in the Guangdong Microbial Culture Collection Center on October 10, 2025, and the preservation number of the chaetomium cupreum DGR-7 is GDMCC No: 67068; according to the invention, the rice culture medium is used for fermenting the A.cupreus strain for the first time, and the A.cupreus strain is separated and purified to obtain 8 compounds, including 6 anthraquinone compounds. An in-vitro antibacterial experiment shows that the secondary metabolite anthraquinone compound of the Arcoporus cupreus DGR-7 has good biological activity in the aspect of inhibiting the growth of staphylococcus aureus, methoxyl-resistant staphylococcus aureus, streptococcus salivarius, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa and escherichia coli.