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Preparation method of hard straw fermentation preparation

The technology of a fermenting bacterial agent and preparation method is applied in the field of preparation of hard straw fermenting bacterial agents to achieve the effect of increasing the fermentation speed

Pending Publication Date: 2017-09-08
GUIZHOU TOBACCO CORP QIANXINAN CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no patent application for hard straw fermentation bacteria

Method used

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  • Preparation method of hard straw fermentation preparation
  • Preparation method of hard straw fermentation preparation
  • Preparation method of hard straw fermentation preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: preparation is used for hard stalk fermentation bacterial agent

[0057] The fungus white rot fungus and the bacteria Bacillus subtilis were selected as the raw material strains for the preparation of hard straw fermentation inoculum; the fungal white rot fungus was purchased from China General Microbiological Culture Collection Center (CGMCC); The bacterium Bacillus subtilis is self-cultured;

[0058] For the selected raw material strains, antagonism tests were carried out on tobacco black shank (Phytophthora parasiticavar.nicotiana) and tobacco bacterial wilt (Pseudomonas solanacearum) to determine their antagonism against major tobacco pathogens; through PCR amplification, DNA sequence determination and analysis Identify the selected active strain molecules; determine its feasibility for the preparation of mixed bacterial agents.

[0059] Then carry out the cultivation of bacteria:

[0060] (1) Cultivation of fungal white rot fungus

[0061] ①Cultiva...

Embodiment 2

[0069] Embodiment 2: one of the effect cases of implementing the hard straw fermentation bacterial agent of the present invention

[0070] The laboratory of the Tobacco and Health Research Center of the University of Science and Technology of China in Hefei, Anhui Province used a fermentation box to verify the implementation effect of white rot fungus: Bacillus subtilis (2:3) on decomposed hard straw (bagasse).

[0071] the experiment shows:

[0072] 1. When white rot fungus: Bacillus subtilis (2:3) is used for the fermentation of a kind of hard straw (bagasse) organic fertilizer, the average fermentation temperature is 33.3°C, which is higher than that of Tiandiyuan fermentation bacteria (powder) (32.4 ℃), and the number of days with a temperature higher than 50 ℃ reaches 15 days, which is 2 days more than Tianjiyuan Fermentation Bacteria (powder).

[0073] 2. White rot fungus: The fermentation agent of Bacillus subtilis (2:3) can promote the growth of fungi, bacteria, actin...

Embodiment 3

[0080] Embodiment 3 Implementation of the Hard Straw Fermentation Bacteria Effect Case Two of the present invention

[0081] In 2016, at the Zhuchangping Organic Fertilizer Factory in Xingyi City, Guizhou Province, large-scale on-site composting of organic fertilizers was used to verify the organic growth of white rot fungus: Bacillus subtilis (2:3) on decomposed hard straw (a mixture of bagasse and tobacco stems). The implementation effect in composting (compared with the commercial bacteria agent with better effect in current production).

[0082] Scale demonstration composting shows that:

[0083] ⑴ During the large-scale composting and fermentation of hard straw (bagasse and tobacco stalks) organic fertilizers, white rot fungus: Bacillus subtilis (2:3) can increase the fermentation temperature (especially the early fermentation temperature) and higher than 50°C The number of days of temperature is conducive to the decomposition and maturity of materials; the average ferme...

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Abstract

The invention discloses a preparation method of a hard strawfermentation preparation. The method comprises the following steps: step one, selecting strains; step two, culturing the strains, includingculturingwhite rot fungi and culturingbacillus subtilis; step three, uniformly mixing the white rot fungi subjected to enlarged culture and the bacillus subtilis subjected to enlarged culture in a mass ratio of 2:3, and loading the white rot fungi subjected to enlarged culture and the bacillus subtilis subjected to enlarged culture into a container subjected to disinfection and sterilization in advance, so as to obtain the hard strawfermentation preparation. A mixed agent obtained through the method is applied to the hard strawfermentation preparation, and can effectively improve the fermentation rate of hard straws, thereby being applied to manufacture of organic fertilizer and servicing agricultural production, and is applicable to recycling of straw in agricultural production.

Description

technical field [0001] The present invention relates to microorganisms, in particular to a fermenting bacterial agent, and further to a straw fermenting bacterial agent. [0002] technical background [0003] Crop straw has high organic matter content, rich in nutrients needed for crop growth, and almost free of impurities. It is a better raw material for organic fertilizer, and the products produced by it have high agricultural value. However, straw has a high carbon content and a high C / N ratio, so it is not suitable as a raw material for organic fertilizer alone, but needs to be used in conjunction with manure with a low carbon content and a low C / N ratio. Generally, the content of lignin, cellulose, and hemicellulose in soft crop straw is relatively low, and it is easy to decompose, such as rice, wheat, rape, corn, etc. The hard straw, such as chaff, sawdust and bark, has a high content of lignin, cellulose and hemicellulose, and the C / N is above 100. It is not easy to d...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20C05F11/08C12R1/645C12R1/125
CPCC12N1/14C12N1/20C05F11/08
Inventor 郭亚利李章海彭宇马莹马骏丁婷齐永霞武丽戴辉刘光快
Owner GUIZHOU TOBACCO CORP QIANXINAN CORP
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