Electrochemical sensor for detecting tyrosine as well as preparation method and application thereof

A quantitative detection method, tyrosine technology, applied in the field of electrochemical sensors for detection of tyrosine and its preparation, can solve the problem that the sensitivity and detection limit cannot meet the requirements of trace detection, low electrocatalytic activity, electrochemical response Weak signal and other issues, to achieve the effect of excellent electrocatalytic activity, simple operation, and low cost

Active Publication Date: 2017-09-12
HENGYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the working electrode used in the electrochemical determination method is used directly without modification, its low electrocatalytic activity wil

Method used

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  • Electrochemical sensor for detecting tyrosine as well as preparation method and application thereof
  • Electrochemical sensor for detecting tyrosine as well as preparation method and application thereof
  • Electrochemical sensor for detecting tyrosine as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of a chemically modified electrode for detecting tyrosine

[0041] (1) Take 50 mg of nitrogen-doped graphene (N-RGO), add 50 mL of water and ultrasonically disperse for 20 minutes to form a solution 1;

[0042] (2) Take 10.423g K respectively 4 [Fe(CN) 6 ] and 0.7455g KCl were added into 10mL HCl and stirred and dissolved to form a solution 2;

[0043] (3) Take 0.162g FeCl respectively 3 and 0.7455g KCl were added into 10mL HCl and stirred to dissolve to form solution 3;

[0044] (4) In a water bath at 35°C, add solution 2 and solution 3 dropwise to solution 1 at a rate of 500 μL / min at the same time, and continue stirring for 30 minutes after the addition is complete;

[0045] (5) Take out the above reaction solution and centrifuge, wash with water 3 times and centrifuge again, dry in an oven at 60°C for 10 hours to get the Prussian blue / nitrogen-doped graphene nanocomposite (PB / N-RGO nanocomposite) ;

[0046] (6) After ultrasonically disper...

Embodiment 2

[0048] Example 2 An electrochemical sensor for detecting tyrosine

[0049] The PB / N-RGO chemically modified electrode prepared in Example 1 was used as a working electrode, Ag-AgCl was used as a reference electrode, and a platinum wire electrode was used as an auxiliary electrode to assemble a three-electrode test system, and connected to an electrochemical workstation for detection Electrochemical sensor of tyrosine.

Embodiment 3

[0050] Example 3 Electrical Performance Test of Chemically Modified Electrodes or Electrochemical Sensors Used to Detect Tyrosine

[0051] (1) Comparison of electron transfer performance of different electrodes

[0052] In the three-electrode test system prepared as in Example 2, the bare glassy carbon electrode (a), the N-RGO modified electrode (b) and the PB / N-RGO chemically modified electrode (c) prepared in Example 1 were used respectively For the working electrode at 5.0mmol / L K 3 [Fe(CN) 6 ] The AC impedance and cyclic voltammetry tests were carried out in the bottom liquid mixed with 0.1mmol / L KCl. AC impedance test conditions are: frequency range 10 5 ~0.1HZ, amplitude 5mV, potential 0.17V; cyclic voltammetry test conditions are: potential range -0.2~0.6V, scanning speed 0.1V / s, the test results are as follows figure 2 . From figure 2 (A) It can be seen that the electrochemical impedance is 1800Ω, 500Ω and 150Ω on the bare electrode, N-RGO modified electrode an...

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Abstract

The invention relates to the technical field of electrochemical detection, and in particular relates to an electrochemical sensor for detecting tyrosine as well as a preparation method and application thereof. The electrochemical sensor adopts a chemically modified electrode for detecting the tyrosine as a working electrode, Ag-AgCl as a reference electrode, and a platinum wire electrode as an auxiliary electrode. An electrode test system is connected with an electrochemical working station to form the electrochemical sensor for detecting the tyrosine after the electrode test system is assembled by utilizing a three-electrode method. The chemically modified electrode or the electrochemical sensor for quantitatively detecting the tyrosine has extremely low detection limit and a wider detection range as well as good stability, anti-interference ability and reproducibility.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to an electrochemical sensor for detecting tyrosine and its preparation method and application. Background technique [0002] Tyrosine is an aromatic amino acid, and it is a raw material for the synthesis of various products in organisms. Although tyrosine is a non-essential amino acid for the human body, it can be transformed into different physiological substances through different metabolic pathways in the body. big help. Tyrosine plays an important role in many fields. For example, in medicine, it can be used to treat symptoms such as polio and thyroid dysfunction, and it can also be used to treat anxiety, depression, skin allergies, headaches, etc., and help melanin The production of vitiligo, reduce symptoms such as vitiligo; at the same time help to prevent cell aging and maintain good health. In the food field, it is often used as a nutritional supplemen...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 李俊华刘梦琴许志锋张复兴耿弘吏贺灵芝
Owner HENGYANG NORMAL UNIV
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