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Preparation method of chitin porous hemostatic microsphere

A chitin and microsphere technology, applied in the fields of medical science, surgery, surgical adhesives, etc., can solve the problems of large consumption of organic solvents and surfactants, high preparation cost, and achieve the effect of fast preparation speed and uniform particle size.

Inactive Publication Date: 2017-09-15
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This preparation method needs to consume a large amount of organic solvents and surfactants, so its preparation cost is high

Method used

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  • Preparation method of chitin porous hemostatic microsphere
  • Preparation method of chitin porous hemostatic microsphere
  • Preparation method of chitin porous hemostatic microsphere

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Preparation of 1.5% chitin solution: Dissolve 11g of sodium hydroxide and 4g of urea in 83.5g of water, then add 1.5g of chitin into the solvent, and stir for 5 minutes at room temperature. The mixed solution was frozen at -30°C for 2 hours, then taken out and stirred until uniform, and then continued to freeze at -30°C. After 4h and 8h, repeat the stirring / freezing process until it dissolves, filter to obtain a clear and transparent liquid, add 0.1g Tween, stir evenly, and store at 4°C for later use.

[0028] 2. Preparation of coagulation solution: Dissolve 25mL of sulfuric acid in 225mL of hydroalcoholic solution (V water: V ethanol = 1:1) as the coagulation solution.

[0029] 3. Preparation of microspheres: inject 20 mL of chitin solution into a 20 mL syringe, and obtain chitin microspheres by electrostatic spraying. Electrostatic spraying conditions are: feed rate: 60 μL / min, voltage: 14 kV.

[0030] 4. Washing and drying: collect the above-mentioned chitin mic...

Embodiment 2

[0033] 1. Preparation of 1.5% chitin solution: Dissolve 11g of sodium hydroxide and 4g of urea in 83.5g of water, then add 1.5g of chitin into the solvent, and stir for 5 minutes at room temperature. The mixed solution was frozen at -30°C for 2 hours, then taken out and stirred until uniform, and then continued to freeze at -30°C. Repeat the stirring / freezing process after 4h and 8h until dissolved, filter to obtain a clear liquid, and store at 4°C for later use.

[0034] 2. Preparation of coagulation solution: Dissolve 25mL of sulfuric acid in 225mL of hydroalcoholic solution (V water: V ethanol = 1:1) as the coagulation solution.

[0035] 3. Preparation of microspheres: inject 20 mL of chitin solution into a 20 mL syringe, and obtain chitin microspheres by electrostatic spraying. Electrostatic spraying conditions are: feed rate: 60 μL / min, voltage: 10 kV.

[0036] 4. Washing and drying: collect the above-mentioned chitin microspheres by filtration, put them into 1M 150mL h...

Embodiment 3

[0038] 1. Preparation of 2.0% chitin solution: Dissolve 11g of sodium hydroxide and 4g of urea in 83g of water, then add 2g of chitin into the solvent, and stir for 5 minutes at room temperature. The mixed solution was frozen at -30°C for 2 hours, then taken out and stirred until uniform, and then continued to freeze at -30°C. After 4h and 8h, repeat the stirring / freezing process until dissolved, filter to obtain a clear liquid, add Tween-80 to make its concentration reach 0.05%, and stir evenly. Store at 4°C for later use.

[0039] 2. Preparation of coagulation solution: Dissolve 25mL of sulfuric acid in 225mL of hydroalcoholic solution (V water: V ethanol = 1:1) as the coagulation solution.

[0040] 3. Preparation of microspheres: inject 20 mL of chitin solution into a 20 mL syringe, and obtain chitin microspheres by electrostatic spraying. Electrostatic spraying conditions are: feed rate: 60 μL / min, voltage: 10 kV.

[0041] 4. Washing and drying: collect the above-mentio...

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Abstract

The invention provides a preparation method of a chitin porous hemostatic microsphere. The method comprises the following steps of spraying a chitin-sodium hydroxide-urea water solution into a solidification liquid through an electrostatic spraying method; carrying out solidification regeneration molding; and carrying out washing and supercritical CO2 drying to obtain the chitin porous hemostatic microsphere, wherein the electrostatic spraying voltage is 5-20kV. Compared with the prior art, the method has the beneficial effects that (1) an organic solvent is not needed, and the method is high in preparation speed, green, environment-friendly and uniform in particle size; and (2) the chitin microsphere has porous structure characteristics, the porosity is 40-60%, the water absorption rate can reach 900-2300%, the specific surface area is 50-130m<2> / g, and the chitin porous hemostatic microsphere can be taken as a fast hemostatic for massive hemorrhage traumatic wounds.

Description

technical field [0001] The invention relates to a preparation method of chitin porous hemostatic microspheres, and belongs to the technical field of application of natural polymer materials. Background technique [0002] Chitin is a renewable natural polymer second only to cellulose in the earth's content. It exists in various crustaceans, molluscs, insects and some algae. As a natural polymer, chitin has excellent characteristics such as biorenewability, biocompatibility, biodegradability and non-toxicity. Compared with its derivative chitosan, it has better structural stability and does not require Deacetylation processing saves energy and is more environmentally friendly. Chitin is widely used in the field of bioengineering, such as three-dimensional cell culture scaffolds, hemostatic membrane / sponge, drug coating and release, anhydrous treatment and food processing, etc., among which, as a hemostatic material, it can significantly reduce the hemostasis time; as a drug p...

Claims

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Application Information

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IPC IPC(8): A61L24/08A61L24/00
Inventor 刘海清涂建炳陈雨枫
Owner FUJIAN NORMAL UNIV
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