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Regeneration utilization method of amino acid production wastewater

A technology for producing wastewater and amino acids, which is applied in microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost of culture medium raw materials, inability to produce fertilizers, and limited use of wastewater. The ecological and economic benefits are remarkable, the utilization efficiency of waste liquid is improved, and the utilization rate is high.

Active Publication Date: 2017-10-03
齐齐哈尔龙江阜丰生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The applicant's previous research was to use waste water as fertilizer, such as the patented technology "Method for preparing fertilizer from amino acid production waste liquid". This technology is suitable for enterprises with fertilizer production qualifications and capabilities, but for other amino acid production enterprises, it cannot The purpose of fertilizer production; and in order to meet the needs of enterprise product diversification, we also need to study other uses of lysine fermentation wastewater
[0004] The Chinese patented technology "Method for producing Debaria hansenii for feeding by fermenting high-salt amino acid wastewater" discloses a method for recycling and utilizing amino acid wastewater to ferment and produce bacterial protein. The basic components are, high-salt amino acid sewage: 125g / L-330g / L, carbon source: 30-100g / L, potassium dihydrogen phosphate (KH 2 PO 4 ): 5-15g / L, yeast powder: 0-10g / L, magnesium sulfate (MgSO 4 ): 3-9g / L, calcium carbonate (CaCO 3 ): 0.75-2g / L, ferrous sulfate (FeSO 4 ): 1.5-4g / L, and the pH of the medium is 3.5-7.5. This method effectively treats wastewater and prepares bacterial protein; however, this method also has some defects: the cost of medium raw materials is high, and the amount of wastewater used is limited, etc. defect

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  • Regeneration utilization method of amino acid production wastewater

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Effect test

Embodiment 1

[0026] A method for recycling waste water from amino acid production, comprising the steps of:

[0027] Step 1) Adjust the degree of acid hydrolysis: first, discharge the waste water from amino acid production into the acid-base adjustment pool, and adjust the pH of the waste water to 6.8;

[0028] Step 2) Prepare acclimatization medium: Take 60g of glucose, 40g of molasses, 40g of yeast powder, MgSO 4 10g, K 2 HPO 4 5g, KH 2 PO 4 5g, MnSO 4 1g, FeSO 4 1g and CaCO 3 1g, add to the wastewater obtained in step 1), adjust the pH to 6.8, and set the volume to 1L;

[0029] Step 3) Microbial acclimatization: Chlorella (1×10 5 individuals / ml) according to 10% (volume ratio) inoculation amount into the acclimatization medium, and cultured at 30°C for 12h; then the Bacillus subtilis seed liquid (1×10 8 individual / ml) and Bacillus licheniformis seed solution (1×10 8 Individuals / ml) were mixed according to the volume ratio of 3:1, and then transferred to the acclimatizati...

Embodiment 2

[0033] A method for recycling waste water from amino acid production, comprising the steps of:

[0034] Step 1) Adjust the degree of acid hydrolysis: first, discharge the waste water from amino acid production into the acid-base adjustment pool, and adjust the pH of the waste water to 6.8;

[0035] Step 2) Prepare acclimatization medium: Take 60g of glucose, 40g of molasses, 40g of yeast powder, MgSO 4 10g, K 2 HPO 4 5g, KH 2 PO 4 5g, MnSO 4 1g, FeSO4 1g and CaCO 3 1g, add to the wastewater obtained in step 1), adjust the pH to 6.8, and set the volume to 1L;

[0036] Step 3) Microbial acclimatization: Chlorella (1×10 5 individuals / ml) according to 10% (volume ratio) inoculation amount into the acclimatization medium, and cultured at 30°C for 12h; then the Bacillus subtilis seed liquid (1×10 8 individual / ml) and Bacillus licheniformis seed solution (1×10 8 Individuals / ml) were mixed according to the volume ratio of 4:1, and then transferred to the acclimatization...

Embodiment 3

[0040] The content of the main components of the amino acid production wastewater used in the present invention is: sugar 18g / L, fatty substance 3g / L, crude protein 15g / L, COD 1600mg / L, ammonia nitrogen 264mg / L and SS 139mg / L.

[0041] Taking Example 1 as an example, the components in the water after the bacteria and algae are collected are: COD 23.4 mg / L, ammonia nitrogen 19.1 mg / L and SS 8.6 mg / L.

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Abstract

The invention belongs to the field of wastewater treatment, and discloses a regeneration utilization method of amino acid production wastewater. The method comprises the following steps of 1, regulating the acidolysis degree; 2, preparing a domestication culture medium; 3, performing microbe domestication; 4, preparing a microbe culture medium; 5, performing culture, filtering and regeneration. The method has the advantages that the degrading capability on various contaminants in the wastewater is good; the yield of microbe protein is greatly improved; the industrial added values are improved.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment, and in particular relates to a recycling method for amino acid production waste water. Background technique [0002] At present, bio-fermentation enterprises produce a large amount of wastewater in the process of fermentation and production of various amino acids, because the fermentation amino acid wastewater contains COD, SS and NH 3 -High N content and strong acidity, etc. In addition, wastewater also contains a large amount of bacterial protein, reducing sugar, etc. If it is discharged randomly, it will not only cause serious environmental pollution, but also waste valuable resources. If the waste water is treated, the investment is huge, and it does not meet the requirements of circular economy development. The comprehensive utilization of amino acid wastewater produced by fermentation is of great strategic significance for the sustainable development of bio-fermentation amino...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12N1/12C02F9/14C12R1/89C12R1/125C12R1/10
CPCC02F1/441C02F1/444C02F1/66C02F3/325C02F9/00C12N1/12C12N1/20C12N1/36
Inventor 唐永强王峰胡俊礼
Owner 齐齐哈尔龙江阜丰生物科技有限公司