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AuNCs@521-MOF (metal-organic framework) nano-sheet compound, preparation method thereof and application

A technology of nanosheets and composites, applied in nanotechnology, nanotechnology, nanotechnology for sensing, etc., can solve problems such as difficult to use on-site testing, complicated operation, expensive equipment, etc., and achieve good physical and chemical stability and Effects of electrochemical activity, good repeatability, and strong bioaffinity

Inactive Publication Date: 2017-10-17
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Several methods have been devised to achieve sensitive detection of cocaine, including surface-enhanced Raman scattering (SERS) spectroscopy, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), enzyme-linked immunosorbent assay ( ELISA), fluorescent immunoassay 5 and rolling circle amplification (RCA). Although these conventional methods can accurately and sensitively detect cocaine, the above methods have several disadvantages, such as complicated operation, troublesome and time-consuming, and expensive equipment so that it is difficult to use in field tests

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  • AuNCs@521-MOF (metal-organic framework) nano-sheet compound, preparation method thereof and application
  • AuNCs@521-MOF (metal-organic framework) nano-sheet compound, preparation method thereof and application
  • AuNCs@521-MOF (metal-organic framework) nano-sheet compound, preparation method thereof and application

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preparation example Construction

[0073] On the one hand, the present invention also provides a preparation method of AuNCs@two-dimensional Zr-MOF nanosheet composite, including:

[0074] uniformly mixing zirconium oxychloride hydrate, trifluoroacetic acid and N,N'-diethylformamide to form a zirconium salt solution;

[0075] 4', 4"', 4"'"-nitrilotris(1,1'-biphenyl-4-carboxylic acid) and N,N'-diethylformamide were uniformly mixed to form an organic solution;

[0076] Mix the zirconium salt solution, organic solution, polyvinylpyrrolidone and ethanol, and stir at 45-60°C for 2-3 days, then separate the solids and wash them, and the two-dimensional zirconium-based metal-organic framework nanosheets are obtained;

[0077] Disperse two-dimensional zirconium-based metal-organic framework nanosheets evenly in DMF, add gold nanoclusters, and stir at 45-60°C for 2-3 days to obtain the AuNCs@two-dimensional Zr-MOF nanosheet composite;

[0078] Preferably, the mass ratio of zirconium oxychloride hydrate, trifluoroacetic...

Embodiment 1

[0106] Preparation of PVP-stabilized two-dimensional 521-MOF

[0107] Zirconia dichloride hydrate (ZrOCl 2 8H 2 O) (0.10 mmol, 35.0 mg) was dissolved in N,N′-diethylformamide (DEF) (1.5 mL) and trifluoroacetic acid (TFA) (0.6 mL) in a 10.0 mL screw cap vial, and the mixture was sonicated for 10 min. Then, 40.0 mg of 4', 4"', 4"'"-nitrilotris(1,1'-biphenyl-4-carboxylic acid) (H 3 A solution of NBB) (0.05mmol) was ultrasonically dissolved in DEF (1.5mL) for 10 minutes; the zirconium salt solution was added to the organic solution to obtain a mixed solution, and then 384.0mg of polyvinylpyrrolidone (PVP) was added to the obtained mixed solution (Mw ≈ 24,000) and sonicate in 3.0 mL of ethanol for 10 min. The above mixture was sonicated for 30 minutes and then stirred at 45-60 °C for 2-3 days. The resulting yellow suspension was centrifuged at 10000 rpm for 3 minutes to pellet it. Finally, the sample was washed with DMF to remove excess precursor, and centrifuged at 10,000 rpm ...

Embodiment 2

[0115] Au electrodes (AE) with a diameter of 3 mm (available from Aida Hengsheng Technology Co., Ltd. (Tianjin, China)) were used and cleaned before use. First, AE was polished using 0.05 mm alumina slurry and a P1 gold polisher, rinsed with ultrapure water (≥18.2Ω.cm) five times, and then washed with piranha solution (H 2 SO 4 :H 2 o 2 volume ratio = 7:3; warning: piranha wash reacts violently with organic solvents) wash AE for 15 min, then wash thoroughly with ultrapure water and dry under nitrogen; finally, in 0.5M H 2 SO 4 AEs were electrochemically washed in solution and under potential cycling conditions between −0.2 and 1.6 V until reproducible cyclic voltammograms were observed, then the activated electrodes were rinsed with ultrapure water and dried under nitrogen .

[0116] Add 5.0 μL of AuNCs@521-MOF nanosheets (prepared in Example 1) aqueous suspension (0.1 mg·mL) dropwise on the activated AE surface -1 ), and then with ultrapure N 2 It was dried; then, the ...

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Abstract

The invention discloses an AuNCs@521-MOF (metal-organic framework) nano-sheet compound, a preparation method thereof and an application. The AuNCs@521-MOF nano-sheet compound comprises a plurality of aggregates formed by two-dimensional zirconium-based metal-organic framework nano-sheets and a plurality of Au nano-clusters distributed in the aggregates. The 2D (two-dimensional) AuNCs@521-MOF nano-sheet compound has high specific surface area, good physicochemical stability and electrochemical activity and high biological affinity for phosphate groups of biological molecules. The invention further discloses an electrochemical aptasensor. The electrochemical aptasensor can simply and conveniently detect cocaine and has the advantages of high selectivity, good repeatability and stability, simplicity in operation and the like, the AuNCs@521-MOF nano-sheet compound can build a new platform for bio-sensing, and the application range of MOFs materials is widened.

Description

technical field [0001] The invention relates to an electrochemical aptamer sensor, in particular to an AuNCs@521-MOF nanosheet complex and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] Because of the urgent need for rapid cocaine detection in law enforcement and clinical diagnostics, cocaine is often used as a representative model target to explore new analytical techniques. As a result, biomedical and psychosocial issues associated with cocaine remain the focus of public attention, and its illicit use remains a major concern for police officers. Several methods have been devised to achieve sensitive detection of cocaine, including surface-enhanced Raman scattering (SERS) spectroscopy, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), enzyme-linked immunosorbent assay ( ELISA), fluorescent immunoassay 5 and rolling circle amplification (RCA). Although these conventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L87/00C08K3/08C08G83/00G01N27/327G01N27/416B82Y30/00B82Y15/00
CPCB82Y15/00B82Y30/00C08G83/008C08K3/08C08K2201/003C08K2201/011G01N27/3277G01N27/3278G01N27/416C08L87/00
Inventor 刘春森张治红杜淼苏方方田稼越赵辉
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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