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Preparation method of pure coenzyme Q10 product

A coenzyme and pure product technology, applied in the field of preparation of pure coenzyme Q10, can solve the problems of high production cost in large-scale production applications, expensive ultrasonic equipment, limited processing capacity, etc., to achieve a simple method, reduce the intensity of environmental protection treatment, and be operable strong effect

Active Publication Date: 2017-11-10
NINGXIA TAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this process, a large amount of heat is released during the ultrasonic treatment process, and freezing and cooling equipment is required. The energy of the ultrasonic wave decays rapidly with the increase of the thickness of the treatment layer, which limits the processing capacity. In addition, the value of ultrasonic equipment is expensive, and the production cost is high for large-scale production applications, so it is not suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 100L of coenzyme Q10 fermentation broth (fermentation unit 2900u / ml), filter it into a ceramic membrane microfiltration membrane (pore size 0.1um), the feed temperature is about 25°C, the dialysis ratio is 1:1.5, and the concentration factor is 1.8 times to obtain 139L of membrane slurry ( Titer 2050u / ml), yield 98.18%.

[0034] The above membrane slurry was spray-dried to control the inlet air temperature of 140-160°C and the outlet air temperature of 60-70°C to obtain 307g of crude coenzyme Q10 with a water content of 9.2% and a yield of 97.90%.

[0035] Spray-dry the above to obtain the crude product, add it to a distiller, add acetone (921ml) 3 times the volume of the crude product, add 0.5‰ (w / v) zeolite, slowly raise the temperature to 49°C, reflux for 2.0hr, and pass through the ceramic while it is hot. The filter element is filtered (pore size 0.5um), and the filter residue is leached again, and the leaching conditions remain unchanged. Collect the leachin...

Embodiment 2

[0039] Take 100L of coenzyme Q10 fermentation broth (fermentation unit 2900u / ml), filter it into a ceramic membrane microfiltration membrane (pore size 0.1um), the feed temperature is about 27°C, the dialysis ratio is 1:1.7, and the concentration factor is 2.0 times to obtain 135L of membrane slurry ( Titer 2130u / ml), yield 99.16%.

[0040] The above film slurry was spray-dried to control the inlet air temperature of 140-160°C and the outlet air temperature of 60-70°C to obtain 308g of crude coenzyme Q10 with a water content of 7.6% and a yield of 98.97%.

[0041] Spray-dry the above to get the crude product, add it to the distiller, add 3.5 times the volume of acetone (1078ml) of the crude product weight, add 0.6‰ (w / v) zeolite, slowly raise the temperature to 50°C, reflux for 2.2 hours, and pass through the ceramic filter element while it is hot Filtrate with a filter (pore size 0.5um), and repeat the leaching of the filter residue once again, and the leaching conditions rem...

Embodiment 3

[0045] Take 100L of coenzyme Q10 fermentation broth (fermentation unit 2900u / ml), filter it into a ceramic membrane microfiltration membrane (pore size 0.22um), the feed temperature is about 29°C, the dialysis ratio is 1:1.8, and the concentration factor is 2.2 times to obtain 127L of membrane slurry ( Titer 2230u / ml), yield 97.87%.

[0046] The above membrane slurry was spray-dried to control the inlet air temperature of 140-160°C and the outlet air temperature of 60-70°C to obtain 301g of crude coenzyme Q10 with a water content of 8.9% and a yield of 96.61%.

[0047] Spray-dry the above to get the crude product, add it to the distiller, add acetone (1204ml) 4 times the volume of the crude product weight, add 0.7‰ (w / v) zeolite, slowly raise the temperature to 51°C, reflux for 2.0hr, and pass through the ceramic while it is hot. Filter the filter element (pore size 0.5um), and repeat the leaching of the filter residue once again, and the leaching conditions remain unchanged. ...

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Abstract

The invention relates to a preparation method of a pure coenzyme Q10 product. The method comprises the following steps: carrying out ceramic membrane microfiltration and spray drying on a coenzyme Q10 fermentation liquid to obtain a crude product, extracting the crude product with acetone, carrying out phase separation and reduced pressure concentration on the obtained extract liquid to obtain a coenzyme Q10 extract concentrate, extracting the coenzyme Q10 extract concentrate with petroleum ether, carrying out silica gel column chromatography, carrying out reduced pressure distillation concentration on the above obtained eluate, adding coenzyme Q10 crystal seeds to crystallize the obtained eluate concentrate, and carrying out reduced pressure drying to obtain the pure coenzyme Q10. Mycelia are crushed through ceramic membrane microfiltration and spray drying, acetone refluxing extraction and ceramic membrane filter separation are carried out to realize wall breaking and separation of mycelium cells, and the coenzyme Q10 is enriched, so the method increases the extraction yield by 7% or more, and facilitates the increase of the economic benefit; the method is simple, and is suitable for industrial production; and the method reduces the wastewater discharge amount, and facilitates environmental protection treatment.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation extraction, in particular to a preparation method of pure coenzyme Q10. Background technique [0002] Coenzyme Q10 (CoenzymeQ10), also known as ubiquinone, molecular formula C 59 h 90 o 4， Molecular weight 863.4, with quinone structure, is a kind of vitamin. Coenzyme Q10 has the functions of scavenging free radicals, improving intracellular respiration, and enhancing immunity. It can be used clinically as an adjuvant therapy for cancer; for the treatment of various heart diseases such as coronary heart disease, heart failure, and arrhythmia; for acute and chronic viral hepatitis, diabetes, loose teeth, muscle weakness, aplastic anemia, gastroduodenal Ulcers, Parkinson's syndrome and other diseases also have auxiliary effects. With the continuous deepening of its application research, coenzyme Q10 is widely used in medicine, food additives, health care and other fields. At pre...

Claims

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Application Information

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IPC IPC(8): C07C46/10C07C50/28
CPCC07C46/10C07C50/28
Inventor 裴立忠李谦张延梅周志伟
Owner NINGXIA TAISHENG BIOTECH CO LTD
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