Preparation method of multi-functional microorganism fish feed

Abstract

The invention relates to a preparation method of a multi-functional microorganism fish feed. The preparation method comprises the following steps of inoculating a liquid nutrient medium with saccharomyces cerevisiae containing recombinant bovine lactoferrin N-terminal peptide plasmid, performing shake culture for 10-24h, then inoculating a fresh culture medium with the cultured saccharomyces cerevisiae, when OD600 is 1.5 to 2.5, inoculating a fermenter with the saccharomyces cerevisiae, and performing fermentation for 60-72h; after the fermentation is finished, performing spray drying on fermentation liquor with a spray drying machine, so as to obtain bovine lactoferricin fermented powder; uniformly mixing fermented cottonseed powder with animal bone powder, corn leaf powder, hoof gelatine powder, shell powder, water and composite microorganisms to obtain a mixture, putting the mixed in a fermenting tank, performing fermentation to obtain a material A, placing the material A in a drying chamber, enabling the material A to stand for 2-5 days, performing crushing and performing screening; uniformly mixing the crushed material A with the bovine lactoferricin fermented powder, trace elements and citric acid on a stirrer to obtain a material B; performing conditioning on the material B with steam of which the pressure is 2-4.5kg until the temperature is 60-66 DEG C, and performing granulation with a granulation machine. The microorganism fish feed provides rich nutrient raw materials for breeding products, can improve water quality and increase the content of dissolved oxygen in water.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a preparation method of multifunctional microbial fish fertilizer. Background technique [0002] Fertilization is the same as traditional pond fish farming in our country, but for fish farming, the purpose of fertilization is mainly to increase the fertility of water, promote the growth and reproduction of various bait organisms, and provide more nutrients for farmed fish. Foodstuffs. Although the existing fish fertilizer has a long-lasting fertilizer effect after application, it decomposes slowly and consumes a large amount of oxygen during decomposition. Improper application may easily cause the death of aquatic animals due to hypoxia. Beneficial microbial preparations have a more obvious promotion effect on water surface fertilization, and they can play a synergistic effect when used at the same time as special fish fertilizer for aquatic products. Some products curren...

AI Technical Summary

Problems solved by technology

The density of fish in the pond is so high that the growth rate of the plankton cannot keep up with the rate at which the fish can eat the biological food, so eventually the plankton cannot grow normally

And the function of existing fish fertilizer is relatively single, a large amount of application will pollute the water quality, can not inhibit the reproduction and growth of pathogenic bacteria in the water body, therefore, lack the effective prevention and treatment of fish disease; at the same time, apply the above-mentioned fertilizer in the fish pond, the water temperature is affected by the external weather change Large, such as "cold in late spring", the water quality is unstable, it is difficult for seedlings to adapt, and the emergency response is strong

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