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Silver nanometer material and biological preparation method and application thereof

A bio-preparation and silver nanotechnology, applied in the field of bio-preparation of silver nanomaterials, can solve problems such as difficult to control slow-release, secondary pollution, poor stability, etc., to achieve good application prospects, good antibacterial effect, particle size Narrow distribution effect

Active Publication Date: 2017-11-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of organic antibacterial materials are poor stability, easy hydrolysis, secondary pollution, toxicity, and difficulty in controlling the slow release

Method used

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  • Silver nanometer material and biological preparation method and application thereof
  • Silver nanometer material and biological preparation method and application thereof
  • Silver nanometer material and biological preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The biological preparation of silver nanomaterials, the preparation process is as follows:

[0040] Fusarium solani D07 strain activation→seed culture solution→fermentation culture→suction filtration to obtain mycelium→mycelia culture in sterile deionized water→suction filtration to obtain filtrate→add AgNO 3 Reaction → material acquisition.

[0041] Concrete preparation process comprises the following steps:

[0042] (1) Take the endophytic fungal strain Fusarium solani D07 of Dendrobium officinale, pick a small amount of hyphae with an inoculation needle under sterile conditions, insert it into a sterilized solid PDA medium test tube, and activate it at 28±1°C 72 hours;

[0043] (2) Take the strain after activation and culture, transfer it into the sterilized liquid PDB seed medium under aseptic conditions, and cultivate it on a shaker at 28±1° C. and 120 rpm for 72 hours to obtain the seed liquid;

[0044] (3) Under sterile conditions, insert 10v% (volume percentage...

Embodiment 2

[0054] The nanomaterial obtained in Example 1 and Comparative Example 1 is carried out to the test observation of antibacterial activity, and the evaluation of antibacterial activity adopts the filter paper method, and the flow process is as follows:

[0055] Activation of pathogenic bacteria strains → seed liquid cultivation → plate coating inoculation → silver nano filter paper sheet inoculation → cultivation → antibacterial effect observation.

[0056] Bacteriostatic activity observation specifically includes the following steps:

[0057] (1) Take 7 kinds of common pathogenic bacteria selected for use: Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella, Bacillus cereus, Candida tropicalis and Alcaligenes strains, under sterile conditions, use Pick up a small number of colonies with the inoculation needle, insert them into a test tube of sterilized beef extract peptone medium (solid state), and activate at 37±1°C for 24 hours;

[0058] (2) Take the strai...

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Abstract

The invention discloses a silver nanometer material and a biological preparation method and application thereof. According to the method, endophytic fungi Fusarium solani D07 (the preservation number is CCTCC No.M2017145) which are isolated from the root of dendrobium candidum are adopted to compound the silver nanometer material in a biological extracellular mode, the reaction condition is mild and easy to control, poisonous or radioactive reagents in chemical and physical methods are avoided, and the advantages of reliability and environmental friendliness are achieved. The silver nanometer material is a round or oval-shaped nanometer particle material which is good in dispersibility and narrow in particle size distribution, the particle diameter is 16-46 nm, and the good antibacterial effect is achieved. By utilizing the good antibacterial activity of the silver nanometer material, the silver nanometer material is applied to preparation of antibacterial materials and has good application prospects.

Description

technical field [0001] The invention relates to the field of preparation and application of silver nanomaterials, in particular to a biological preparation method and application of silver nanomaterials. Background technique [0002] Due to their potential applications in optoelectronics, catalysis, sensors and biology, metal nanomaterials have become one of the important research directions in the past ten years. Among them, nano-silver, as an emerging functional material, has attracted much attention in the research fields of nano-optoelectronic devices, biomarkers, surface-enhanced Raman and biomedical composite materials due to its unique physical and chemical properties. [0003] Antibacterial materials can be divided into three types: natural, organic and inorganic antibacterial materials. Natural biological antibacterial materials are derived from natural extracts, are non-toxic or low-toxic, but have poor persistence and are limited by processing conditions, so they...

Claims

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Application Information

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IPC IPC(8): C12P3/00A01N59/16A01P1/00C12R1/77
CPCA01N59/16C12P3/00
Inventor 娄文勇王洪峰曾英杰宗敏华蒲磊
Owner SOUTH CHINA UNIV OF TECH
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