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Bacterial cellulose/polyvinyl alcohol medical composite material and preparation method thereof
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A technology of bacterial cellulose and polyvinyl alcohol, applied in the field of preparation of biomedical materials, can solve the problems of destroying the three-dimensional network structure of bacterial cellulose, complex process, complicated steps, etc., and achieve enhanced phase interface interaction and strong biological stability , the effect of low fermentation temperature
Inactive Publication Date: 2017-11-21
EAST CHINA UNIV OF SCI & TECH
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The pouring method needs to crush the bacterial cellulose in advance, the process is complicated, and it will destroy the three-dimensional network structure of the bacterial cellulose; the current research results mainly focus on the impregnation method.
Jie hua wang et al. (Wang J, Gao C, Zhang Y, et al. Preparation and in vitro characterization of BC / PVA hydrogel composite for its potential use as artificial corneabiomaterial[J]. Materials Science & Engineering C, 2010, 30(1) : 214-218.) Immerse the prepared bacterial cellulose wet film in the polyvinyl alcohol aqueous solution, and freeze-thaw and cross-link it to make the content of water and the cornea close to the bacterial cellulose / polyvinyl alcohol composite material, but complicated steps
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Embodiment 1
[0025] (1) take by weighing 2g polyvinyl alcohol, dissolve with deionized water at 80 DEG C,
[0026] (2) Add 7.0g of glucose and 1.5g of yeast extract into the beaker, stir well to dissolve, add 1.0mL of absolute ethanol, set the volume to 100mL, and adjust the pH of the solution to 6.8. Put it into an Erlenmeyer flask, seal it, and sterilize at 121°C for 20 minutes. After inoculation, culture it at a constant temperature at 20°C for 10 days to obtain a bacterial cellulose / polyvinyl alcohol composite material wet film. Put the composite membrane in 0.1M NaOH solution, let it stand at room temperature for 24 hours, wash it with a large amount of deionized water until the surface of the fiber is neutral,
[0027] (3) drying the composite material film obtained in step (2) in an oven at 30° C. to obtain a bacterial cellulose / polyvinyl alcohol medical composite dry film.
[0028] According to the light transmittance test, when the concentration of polyvinyl alcohol is 20g / L, the...
Embodiment 2
[0030] Weigh 3g of polyvinyl alcohol, dissolve it with deionized water at 80°C, and then prepare the bacterial cellulose / polyvinyl alcohol composite material with steps (2) and (3) in Example 1.
Embodiment 3
[0032] (1) Take 2g polyvinyl alcohol, dissolve it with deionized water at 80°C,
[0033] (2) Add 7.0g of glucose and 1.5g of yeast extract into the beaker, stir well to dissolve, add 1.0mL of absolute ethanol, set the volume to 100mL, and adjust the pH of the solution to 6.8. Put it into an Erlenmeyer flask, seal it, and sterilize at 121°C for 20 minutes. After inoculation, culture it at a constant temperature at 30°C for 10 days to obtain a bacterial cellulose / polyvinyl alcohol composite material wet film. Put the membrane in 0.1M NaOH solution, let it stand at room temperature for 24 hours, wash it with a large amount of deionized water until the surface of the fiber is neutral,
[0034] (3) Dry the composite material film obtained in step (2) in an oven at 40° C. to obtain a bacterial cellulose / polyvinyl alcohol medical composite dry film.
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technical field [0001] The invention relates to the preparation of a biomedical material, in particular to a bacterial cellulose / polyvinyl alcohol medical composite material and a preparation method thereof. Background technique [0002] Bacterial Cellulose (BC for short) is a natural cellulose produced by microbial metabolism, and its chemical structure is the same as that produced by plants and seaweed. Bacterial cellulose has high mechanical strength, high crystallinity, high water holding capacity, good biocompatibility, biodegradability, does not contain any impurities and has ultra-fine nanofiber network. Thanks to the many excellent properties of bacterial cellulose, bacterial cellulose is widely used in many fields. In terms of biomedical materials, bacterial cellulose can be used as medical dressings, artificial skin, artificial blood vessels, tissue engineering scaffolds, artificial cartilage, etc., and has Good application prospects. Although bacterial cellulose...
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