Application of cucurbitacin in the preparation of anti-atherosclerotic drugs
A technology for atherosclerosis and cucurbit tea, applied in the field of medicine, can solve the problems of less research on the prevention and treatment of cardiovascular and cerebrovascular diseases, and achieve the effects of less toxic and side effects, definite curative effect and clear ingredients.
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Embodiment 1
[0031] Preparation of Cucurbitacin
[0032] Gourd tea medicinal material, add 8000ml of ethanol with a volume concentration of 40-95% and heat and reflux to extract, recover the ethanol from the extract until it has no alcohol smell, extract the alcohol extract with petroleum ether, and obtain a petroleum ether layer and a water layer; extract the water layer with ethyl acetate , to obtain an ethyl acetate layer and a water layer; the water layer was extracted with n-butanol to obtain a n-butanol extract, which was recovered and dried to obtain 50 g of cucurbit tea n-butanol extract. Take the n-butanol extract of gourd tea, dissolve it in an organic solvent such as methanol, ethanol, etc., mix it with column chromatography silica gel (200-300 mesh), and perform silica gel column chromatography separation. After the silica gel column chromatography is filled, use dichloromethane-acetone or methanol gradient elution (100:0; 90:10; 80:20), and prepare three gradient elution fract...
Embodiment 2
[0036] Weigh 10 g of cucurbitacin in Example 1, add 6 g of starch, 4 g of microcrystalline cellulose, and 0.1 g of magnesium stearate, mix well, and prepare tablets, capsules or granules by methods known in the art.
Embodiment 3
[0038] Weigh 10 g of cucurbitacin in Example 1, dissolve it in 12 ml of ethanol, add 2 g of hydroxypropyl methylcellulose (HPMC), stir and evaporate the solvent on a water bath, dry in a vacuum oven at 50° C. for 3 h, pulverize, and pass 30 Mesh sieve, fill capsules to obtain capsules.
[0039] Pharmacodynamic study of cucurbitacin on atherosclerosis
[0040] ApoE- / - mice were operated on the left common carotid artery, and were divided into 5 groups at 28 days:
[0041] (1) AS model group (high-fat diet operation group, n=12)
[0042] (2) AS model + low concentration cucurbitacin group (administration of 5 mg / kg after surgery with a high-fat diet, n=12)
[0043] (3) AS model + medium-concentration cucurbitacin group (20 mg / kg administered after surgery with high-fat diet, n=12)
[0044] (4) AS model + high-concentration cucurbitacin group (administration of 80 mg / kg after surgery with high-fat diet, n=12)
[0045](5) AS model + atorvastatin group (20 mg / kg administered af...
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