Preparation method of composite preparation for preventing and treating poultry respiratory diseases
A compound preparation and respiratory technology, applied in the direction of respiratory diseases, antiviral agents, medical preparations containing active ingredients, etc., can solve the problems of poor clinical immune prevention effect and no specific drugs for treatment, and achieve the prevention of infectious bronchial inflammation, reduce drug residues, and ensure safety
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[0032] Example 1: Preparation of extracts and powders of Chinese veterinary medicine
[0033] The inventors screened and clinically validated Chinese veterinary medicines for the treatment of respiratory diseases in poultry, and determined that the basic prescription is Yemazhu, and most preferably Yemazhu, Isatis indigotica, Ephedra, Platycodon grandiflorum, Andrographis paniculata, Houttuynia cordata, Astragalus, Tingliazi and Poria cocos The mass ratio is 1-10:1:1:1:1:1:1:1:1. Ultrasonic technology and vacuum freeze-drying process were used to concentrate and extract the components of Chinese medicine to prepare Chinese veterinary medicine extracts.
[0034] After the wild horse is dried, pulverized with a micro pulverizer and mixed, placed in a cork with a cork, add 5 times the volume of the extraction solvent ethyl acetate, and then use ultrasonic to extract its active ingredients, soaking time before extraction is 60- 90min. The ultrasonic frequency is 20-40kHz, ultrasonic...
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[0038] Example 2: Preparation of chicken egg yolk immunoglobulin powder
[0039] Choose healthy laying hens and use commercially-available or self-made respiratory pathogenic microbial monovalent or multivalent vaccines (such as Newcastle disease-infectious bronchitis-avian influenza triple inactivated vaccine or Newcastle disease-infectious bursa-infectious bronchitis triple (Vaccine) Multiple injections into the chest muscles of laying hens, 0.5 mL / bird, immunized once a week, after three immunizations, after the immunoglobulin level reaches the high titer, collect the eggs.
[0040] To extract egg yolk immunoglobulin (taking the acetic acid-sodium acetate buffer method as an example), the eggs are first washed with water to remove the dirt on the surface of the egg shell, immersed in 0.5% neocervical solution, soaked for 10 minutes, and then rinsed with 75% alcohol. Break the eggshell aseptically, pour it into a sterilized container, separate the egg yolk with a sterilization s...
Example Embodiment
[0043] Example 3: Selection of the best ratio of Chinese veterinary drug yolk immunoglobulin compound preparation
[0044] According to different ratios, the best ratio of Chinese veterinary medicine and chicken egg yolk immunoglobulin was studied.
[0045] According to a completely random design, 200 5-week-old unimmunized AA broilers were randomly divided into 4 test groups and 1 control group, with 40 chickens in each group. According to the different proportions (mass ratio) of the Chinese veterinary medicine extract powder described in Example 1 and the egg yolk immunoglobulin described in Example 2, they were divided into test groups I, II, III and IV, and the proportions were 1:1. , 1:0.8, 1:0.5, 1:0.2. Each chicken in the experimental group was administrated with 1.5 ml of the aqueous solution of the Chinese veterinary drug yolk immunoglobulin compound preparation, and the control group was administrated with 1.5 ml of normal saline as a placebo.
[0046] The experiment sh...
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