A kind of cross-linked polyglutamic acid gel microsphere suspension for injection and preparation method thereof
A technology of polyglutamic acid gel and polyglutamic acid, which is applied in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of high injection resistance, high solution viscosity, and bursting of syringes in filling injections, and achieve bolus injection The effect of small resistance, small particle size, and reduced injection resistance
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Embodiment 1
[0022] Weigh 5 g of polyglutamic acid, add 100 mL of distilled water to dissolve to obtain polyglutamic acid aqueous solution. Take 10mL of Span 80 and 30ml of Tween 80 respectively, put them into 160mL of liquid paraffin, stir and mix well. Add polyglutamic acid aqueous solution into liquid paraffin containing mixed emulsifier, and make polyglutamic acid microspheres through membrane emulsification. Observation by oil microscope, the polyglutamic acid gel microspheres are spherical. When more than 90% of the microspheres have a particle size of <5 μm, add 0.25 mL polyethylene glycol glycidyl ether, heat at 45 °C and stir at 50 rpm for 12 hours to cross-link. The liquid layer was removed by centrifugation, washed successively with petroleum ether, acetone and ethanol, and finally the cross-linked polyglutamic acid microspheres were suspended in 0.9% saline to obtain a suspension of cross-linked polyglutamic acid gel microspheres, which could be Injects smoothly through a 30G ...
Embodiment 2
[0024] Weigh 25 g polyglutamic acid, add 100mL distilled water to dissolve to obtain polyglutamic acid aqueous solution. Take 30mL of Span80 and 10mL of Tween 80 respectively to make emulsifier. Take 1000mL of liquid paraffin and 60mL of petroleum ether with a boiling range of 60°C to 90°C, then add the prepared emulsifier, stir and mix well. The polyglutamic acid aqueous solution is added to the oil phase containing emulsifier, and the polyglutamic acid microspheres are prepared by emulsifying through a homogenizer. Observation by oil microscope, the polyglutamic acid gel microspheres are spherical, when more than 90% of the microspheres have a particle size <5 μm, add 10 mL of ethylene glycol glycidyl ether, heat at 75 °C and stir at 500 rpm for 4 h to cross-link. The liquid layer was removed by centrifugation, washed with chloroform and ethanol in turn, and finally the cross-linked polyglutamic acid microspheres were suspended in physiological saline containing 0.5% procai...
Embodiment 3
[0026] Weigh 12g polyglutamic acid, add 100mL distilled water to dissolve to obtain polyglutamic acid aqueous solution. Take 30mL of Span 80 and 30mL of Tween 80 respectively to make emulsifier. Take 1000mL of liquid paraffin, add 40mL of petroleum ether with a boiling range of 60°C to 90°C, then add the prepared emulsifier, stir and mix well. The polyglutamic acid aqueous solution is added to the oil phase containing emulsifier, and the polyglutamic acid microspheres are prepared by emulsifying through a homogenizer. Observation by oil microscope, the polyglutamic acid gel microspheres are spherical, when more than 90% of the microspheres have a particle size of <5 μm, add 2.4mL ethylene glycol glycidyl ether, heat at 55°C and stir at 100rmp for 10h to cross-link. The liquid layer was removed by centrifugation, washed with chloroform and ethanol in turn, and finally the cross-linked polyglutamic acid microspheres were suspended in physiological saline containing 0.5% procain...
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