Method for extracting anthocyanin from plant

An anthocyanin and plant technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of uncoordinated use of solvents, difficulties in separation and purification, and large differences in organic solvents, and achieve atom utilization 100%, the raw material is cheap and easy to get, and the effect of increasing the adsorption effect

Active Publication Date: 2017-11-24
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complex preparation steps, low extraction efficiency, large differences in the organic solvents used by anthocyanins of different polarities, and solvent toxicity and volatility, it is very easy to cause environmental pollution and organic solvent residues in the extract.
[0004] The emergence of ionic liquids was once considered to be a good substitute for traditional organic solvents, but with the deepening of research, people gradually found that these solvents were in some irreconcilable contradictions in the pro

Method used

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  • Method for extracting anthocyanin from plant
  • Method for extracting anthocyanin from plant
  • Method for extracting anthocyanin from plant

Examples

Experimental program
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Embodiment approach 1

[0027] This embodiment provides a method for extracting anthocyanins from mulberries. Take fresh mulberry fruits, remove the stems, beat them into a homogenate with a mixer, take 10 g of the homogenate and use the deep eutectic solvent choline chloride-lactic acid (molar ratio 1: 3, water content 0%wt) as the extraction solvent, extract for 10 minutes under the conditions of solid-to-liquid ratio 1g: 30mL, ultrasonic power 100W, electrostatic field strength 4kV, extraction temperature 50°C to obtain anthocyanin extract; add the above anthocyanin extract Add 5g of attapulgite-loaded chitosan cross-linked cyclodextrin complex, shake it on a shaking table at 30°C and 100r / min for 4 hours, take the precipitate after centrifugation, wash it with deionized water ultrasonically for 3 times, and centrifuge to get the adsorbed on the attapulgite Anthocyanin precipitation on the soil-loaded chitosan cross-linked cyclodextrin complex; add 80% ethanol to the anthocyanin precipitation for u...

Embodiment approach 2

[0039] This embodiment provides a method for extracting anthocyanins from mulberries. Take fresh mulberry fruits, remove the stems, beat them into a homogenate with a mixer, take 10 g of the homogenate and use the deep eutectic solvent choline chloride-acetic acid (molar ratio 2: 3, the water content is 15%wt) as the extraction solvent, and the solid-to-liquid ratio is 1g: 10mL, the ultrasonic power is 300W, the electrostatic field strength is 5kV, and the extraction temperature is 50°C, and the anthocyanin extract is obtained for 20min; the above anthocyanin extract Add 3 g of attapulgite-loaded chitosan cross-linked cyclodextrin complex, shake it on a shaking table at 30°C and 100 r / min for 5 hours, centrifuge to get the precipitate, wash it with deionized water ultrasonically for 3 times, and centrifuge to get the adsorbed on the attapulgite Anthocyanin precipitation on the soil-loaded chitosan cross-linked cyclodextrin complex; add 60% ethanol to the anthocyanin precipitati...

Embodiment approach 3

[0042] This embodiment provides a method for extracting anthocyanins in mulberries. Take fresh mulberry fruits, remove the stems, beat them into a homogenate with a mixer, take 10 g of the homogenate and use the deep eutectic solvent choline chloride-lactic acid-acetic acid (molar ratio 1:2:1, water content 30%wt) as the extraction solvent, under the conditions of solid-liquid ratio 1g:20mL, ultrasonic power 400W, electrostatic field strength 9kV, extraction temperature 60°C for 30min to obtain anthocyanin extract; Add 2 g of attapulgite-loaded chitosan cross-linked cyclodextrin complex to the obtained anthocyanin extract, shake it on a shaking table at 30°C and 100 r / min for 5 hours, centrifuge to collect the precipitate, and wash it with deionized water ultrasonically for 3 times. Centrifuge to obtain the anthocyanin precipitate adsorbed on the attapulgite-loaded chitosan cross-linked cyclodextrin complex; add ethanol with a mass fraction of 70% to the anthocyanin precipitate...

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Abstract

The invention relates to the field of edible pigment and discloses a method for extracting anthocyanin from a plant. The method comprises the following steps: pulverizing the plant containing the anthocyani and adding a deep-eutectic solvent; extracting anthocyanin extracting liquid by an electrostatic field ultrasonic extraction method; adding an attapulgite loaded chitosan crosslinked cyclodextrin compound into the anthocyanin extracting liquid to obtain an anthocyanin compound precipitate adsorbed on the attapulgite loaded chitosan crosslinked cyclodextrin compound; dissolving the anthocyanin compound precipitate in an organic solvent to perform desorption and centrifugation to obtain anthocyanin supernatant containing the organic solvent; enabling the anthocyanin supernatant to pass through a microporous filtration membrane to obtain a purified anthocyanin solution containing the organic solvent; and removing the organic solvent from the anthocyanin solution and removing the redundant water to obtain a target product anthocyanin. The deep-eutectic solvent serves as an extracting solvent, so the method is green and environment-friendly, and the extraction rate is high; and the anthocyanin is purified and separated by the attapulgite loaded chitosan crosslinked cyclodextrin compound, so the process is simple and the purification and separation efficiency is high.

Description

technical field [0001] The invention relates to the technical field of edible anthocyanins extraction, in particular to a method for extracting plant red / purple pigments. Background technique [0002] Anthocyanin (Anthocyanin) is a kind of natural pigment widely present in the cell fluid of plant roots, stems, leaves, fruits and other organs. flavonoid polyphenols. As a natural pigment, anthocyanins are safe, non-toxic, rich in resources, and have many health care effects on people, such as scavenging free radicals in the body, scavenging DPPH, anti-inflammatory, anti-cancer, delaying aging, preventing diabetes, and protecting eyesight. Therefore, anthocyanins are now widely used in medical, beauty, food and other industries. [0003] Anthocyanins, which constitute natural plant pigments, are often composed of multiple complexes with different polarity and physical and chemical properties, so it is difficult to prepare them in one step with a single solvent in the traditio...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/065C09B61/00B01J20/24B01J20/30
CPCB01J20/24C07H1/08C07H17/065C09B61/00
Inventor 王朝宇丁成新张文静毕艳红杨荣玲
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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