Preparation and application of a fluorescent sensor capable of detecting endogenous glutathione

Abstract

The invention discloses preparation and application of a water-soluble fluorescence sensor for detecting endogenous glutathione (GSH) in a targeting manner based on cancer cells (Hela cells). The water-soluble fluorescence sensor for detecting endogenous glutathione in a targeting manner based on the cancer cells is prepared from a manganese dioxide nanosheet, a block polymer and a dye, wherein the manganese dioxide nanosheet and the block polymer are prepared according to the prior art, and the dye has aggregation-induced emission (AIE) performance. By utilizing the water-soluble fluorescence sensor, the high-selectivity and high-sensitivity detection of endogenous glutathione in the cancer cells can be realized. Compared with an existing fluorescence detection technique, the water-soluble fluorescence sensor has the advantages that the high-selectivity rapid response to glutathione is realized, the cytotoxicity is low, the water dispersibility is excellent, the input cost is relatively low, the synthetic route is simple, and the like; and the water-soluble fluorescence sensor is applicable to amplification synthesis and practical production application and has huge application prospects in the technical fields of analytical chemistry, life sciences and the like.

Description

Technical field [0001] The invention relates to the preparation and application of a fluorescent sensor that can be used to detect glutathione, in particular to the preparation and application of a water-soluble fluorescent sensor that can detect endogenous glutathione in cancer cells, and belongs to The field of chemical material preparation and analysis and testing. Background technique [0002] Glutathione (GSH) is a very important reducing species widely found in biological cells. It is also one of the essential physiologically functional amino acids of the human body. It plays an important role in maintaining the redox process of cells. Abnormal biological GSH is associated with many diseases, such as cancer, liver damage, aging, diabetes, HIV, AIDS, Parkinson's disease, and children with autism. Especially in cancer cells, the level of GSH is 2 to 4 times higher than that in normal cells. Therefore, it is of great significance to be able to perform imaging analysis and de...

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Problems solved by technology

[0004] In addition, for glutathione (GSH), many specific oxidation fluorescent sensors based on manganese dioxide nanosheets have been prepared, such as carbon quantum dots (Liu, J, et al. J Agric Food Chem 2016, 64(1), 371-380; Das, K.et al.ACS Appl Mater Interfaces 2016, 8(39), 25691-25701; CN104597019 A), graphene quantum dots (Yan, X.et al.ACS Appl Mater Interfaces 2016,8(34),21990-21996.), small organic molecules (Dong,Z.et al.Nanoscale 2017,9(14),4677-4682.), silicon nanoparticles (Meng,H.M.et al.Anal Chem 2014,86(24),12321-12326; Meng,H.M.et al.AnalChem 2015,87(8),4448-4454.), upconversion nanoparticles (Yuan,J.et al.ACSAppl.Mater.Interfaces 2015 ,7,10548-10555), but there are still several problems, firstly, these sensors that have been reported are all simple cell imaging, and do not target endogenous GSH imaging and detection of cancer cells; secondly, whether carbon quantum dots , silicon nanoparticles, or small molecules, all have a common defect, that is, when the concentration increases to a certain level, there will be aggregation-induced fluorescence quenching effect (ACQ); for these materials, they cannot be normalized by organisms in vivo. Metabolism, a potential threat to normal organisms

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