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Mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold, curcumin-carrying scaffold, as well as preparation methods and applications

A technology of mesoporous calcium magnesium silicate and wheat protein, which is used in medical science, prosthesis, tissue regeneration, etc., can solve the problems of excessive degradation rate, low mechanical strength, and influence on osteogenic performance, and achieves good repair effect. The effect of good biological activity and suitable degradation rate

Inactive Publication Date: 2017-12-05
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing mesoporous calcium magnesium silicate / wheat protein composite material that the mechanical strength is still low and the degradation rate is too fast, which affects the osteogenic performance, and provides a mesoporous calcium silicate Magnesium-genipin cross-linked wheat protein (m-GCWG) scaffold, curcumin-loaded scaffold, preparation method and application

Method used

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  • Mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold, curcumin-carrying scaffold, as well as preparation methods and applications
  • Mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold, curcumin-carrying scaffold, as well as preparation methods and applications
  • Mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold, curcumin-carrying scaffold, as well as preparation methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] 1. Preparation of mesoporous calcium magnesium silicate (m-MCS)

[0087] The m-MCS of this embodiment is prepared by the following method:

[0088] S1. Weigh 6g template agent P123 triblock copolymer, add 217mL deionized water and 36mL ethanol, and keep stirring until the solution becomes clear;

[0089] S2. In a water bath at 38°C, 17mL HCl (2.0mol / L) and 12.9mL TEOS were slowly added dropwise to the solution, and then 7.9g Mg(NO 3 ) 2 ·6H 2 O, 7.3g Ca(NO 3 ) 2 4H 2 0 and 30mL deionized water, during this process, continue to keep the water bath at 38°C, and continue to stir the solution for 5h to react to form a sol;

[0090] S3, aging the obtained sol at 95° C. for 3 days to precipitate the gel substance;

[0091] S4. The obtained precipitate was washed successively with deionized water and absolute ethanol, and then calcined at 600°C to remove the template agent P123 tri-block copolymer to obtain final product.

[0092] 2. Preparation of Mesoporous Calcium M...

Embodiment 2

[0098] 1. Preparation of mesoporous calcium magnesium silicate (m-MCS)

[0099] The preparation method of m-MCS in this example is the same as that in Example 1.

[0100] 2. Preparation of Mesoporous Calcium Magnesium Silicate-Genipin Crosslinked Wheat Protein (m-GCWG) Scaffold

[0101] The m-GCWG scaffold of this embodiment is prepared by the following preparation method, and the schematic diagram of the preparation process can be found in the attached figure 1 :

[0102] (1) Mix m-MCS and WG according to the mass ratio of 40:60, and add porogen NaCl particles (d≈400-500nm) that are 6 times the total mass of m-MCS and WG, mix and stir evenly, Obtain solid mixture;

[0103] (2) Place the obtained solid mixture in a stainless steel mold (Φ12×2mm), press it with a powder tablet press under a pressure of 2MPa, and keep the pressure for 3min, and then put each formed sample into a 24-hole plate, Add a genipin solution with a concentration of 10 mmol / L and a volume of 1 mL to e...

Embodiment 3

[0106] 1. Preparation of mesoporous calcium magnesium silicate (m-MCS)

[0107] The m-MCS of this embodiment is prepared by the following method:

[0108] S1. Weigh 5g template agent P123 triblock copolymer, add 170mL deionized water and 29mL ethanol, and keep stirring until the solution becomes clear;

[0109] S2. In a water bath at 35°C, slowly add 14mL HCl (1.5mol / L) and 10mL TEOS dropwise into the solution, and then add 6.5g Mg(NO 3 ) 2 ·6H 2 O, 5.8g Ca(NO 3 ) 2 4H 20 and 25mL deionized water, during this process, continue to keep the water bath at 35°C, and continue to stir the solution for 4h to react to form a sol;

[0110] S3, aging the obtained sol at 90° C. for 3 days to precipitate the gel substance;

[0111] S4. The obtained precipitate was washed successively with deionized water and absolute ethanol, and then calcined at 500°C to remove the template agent P123 tri-block copolymer, to obtain final product.

[0112] 2. Preparation of Mesoporous Calcium Magn...

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Abstract

The invention discloses a mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold, a curcumin-carrying scaffold as well as preparation methods and applications. The preparation method of the mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold comprises the following steps: (1) mixing mesoporous calcium silicate magnesium, wheat protein and a pore-forming agent to obtain a solid mixed material; (2) carrying out compression molding on the solid mixed material, carrying out mixing crosslinking with genipin solution with the concentration being 6-14 mmol / L, thus obtaining a precursor; and (3) soaking the precursor in a solvent to remove the pore-forming agent, and carrying out freeze-drying, thus obtaining the mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold. The preparation method of the curcumin-carrying scaffold comprises the following step: immersing the scaffold in curcumin solution for adsorbing curcumin, thus obtaining the curcumin-carrying scaffold. The mesoporous calcium magnesium silicate-genipin-wheat protein cross-linked scaffold provided by the invention has the excellent biological activity and degradability, can promote cell adhesion, proliferation and differentiation, and has good mechanical property and bone repair effect, after curcumin is loaded, the scaffold has certain antibacterial performance, and meanwhile, no obvious inhibition effect is caused to osteogenesis.

Description

technical field [0001] The invention relates to a mesoporous calcium magnesium silicate-genipin crosslinked wheat protein scaffold, a curcumin-loaded scaffold, a preparation method and an application. Background technique [0002] The main components in bone are collagen and apatite, of which 10-30% are hard and porous outer structure (cortical bone), and 30-90% are elastic porous inner structure (cancellous bone). The performance varies widely, so designing a scaffold with natural bone structure is a very difficult task. Among them, scaffold materials play an extremely important role in the treatment of bone defects. [0003] There are usually two types of materials for repairing bone defects in the past: the first type is bioinert ceramics (Al 2 o 3 , Si 3 N 4 Ceramics, etc.) and medical-grade metals and alloys, which do not or only have weak chemical reactions in the body; the second category is bioactive materials, which can induce new bone tissue and reduce immune ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/54A61L27/56
CPCA61L27/446A61L27/54A61L27/56A61L2300/216A61L2300/404A61L2300/412A61L2300/604A61L2430/02C08L89/00
Inventor 魏杰魏武高超梅师奇胡兴龙韩航蔡亮苏佳灿冯世鹏
Owner EAST CHINA UNIV OF SCI & TECH