Preparation of interface self assembled carbonyl reductase and application thereof to S-eslicarbazepine synthesis

Abstract

The invention discloses preparation of interface self assembled carbonyl reductase and application thereof to S-eslicarbazepine synthesis. The interface self assembled carbonyl reductase is prepared by the following method that carbonyl reductase is added into a buffer solution; then, a methylbenzene solution with dissolved polystyrene is added; oscillation reaction is performed for 2 hours in a shaking bed under the conditions of dark environment, 80r / min and 30 DEG C; centrifugation is performed; a reaction system with the upper layer being an organic phase, the interface self assembled carbonyl reductase distributed in the oil water interface, and the lower layer being a water phase is obtained; the interface self assembled carbonyl reductase is obtained; the interface self assembled carbonyl reductase has a good catalysis function in the oil and water two-phase interface; the oxcarbazepine can be converted into S-eslicarbazepine; the enantiometric excess value of the S-eslicarbazepine is greater than 98 percent. In the water phase, the coenzyme NADH can smoothly realize the in-situ regeneration; the substrate conversion rate can be favorably improved. The interface self assembled enzyme can realize the multi-time repeated utilization, the advantage is incomparable by free enzymes.

Description

(1) Technical field

[0001] The invention relates to the preparation of a microbial-derived interface self-assembly carbonyl reductase and its application in the synthesis of S-licarbazepine, a key chiral intermediate of the antiepileptic drug eslicarbazepine acetate. (2) Background technology

[0002] Interfacial self-assembly enzymes are polymer-enzyme complexes, which combine enzymes with macromolecular polymers to make the modified enzymes have an amphiphilic structure, which is similar to protein-based surfactants, containing both hydrophilic The protein head also contains a hydrophobic polymer tail. When it is placed in an oil-water system, the polymer part of the polymer-enzyme extends to the oil phase, while the enzyme part is embedded in the water phase, immobilizing the enzyme in the oil phase. - Catalyzes chemical reactions in the oil-water two-phase at the water interface. Combining carbonyl reductase and polymer at a suitable oil-water interface to form an inter...

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