Celebral dura mater sealing hydrogel and a preparation method and application thereof
A gel and mixture technology, applied in the field of albumin-encapsulated gel and its preparation, can solve the problems of brain tissue compression, low bursting strength, and difficulty in observation, and achieve the effect of improving reaction speed and mechanical properties
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0051] Cerebrospinal fluid leaks are a common complication after head and spine surgery. Because the nerves near the cranium and spine are sensitive to compression from tissue inflammation or swelling from surgically implanted materials such as artificial dura mater, hydrogels with low swelling properties are required to minimize tissue compression . The preparation method of the sealing hydrogel for preventing cerebrospinal fluid leakage of the present invention is as follows:
[0052] Weigh 2.5 g of human serum albumin (25%, w / v) and dissolve it in 10 mL of phosphate buffer (10 mM, pH=9.2) prepared from deionized water. Stir intermittently at 37°C for 1 hour to dissolve the protein. After the protein is completely dissolved and becomes a transparent liquid, use a vacuum pump for 30 minutes to quickly eliminate the bubbles in the solution.
[0053] Weigh 2 g of 4-arm polyethylene glycol succinimide glutarate (4-arm-PEG-SG, molecular weight 10,000 Da) and 5 mg of FD&C blue #...
Embodiment 2
[0056] Weigh 2 g of human serum albumin (20%, w / v) and dissolve it in 10 mL of phosphate buffer (10 mM, pH=9.2) prepared from deionized water. Stir intermittently at 37°C for 1 hour to dissolve the protein. After the protein is completely dissolved and becomes a transparent liquid, use a vacuum pump for 30 minutes to quickly eliminate the bubbles in the solution.
[0057] Weigh 2 g of 4-arm polyethylene glycol succinimide glutarate (4-arm-PEG-SG, molecular weight 20,000 Da) and 10 mg of FD&C blue #2 visualization reagent, dissolve in 10 mL of phosphoric acid prepared by deionized water Salt buffer solution (10mM, pH=7.4), vortex to dissolve it into a transparent mixture liquid.
[0058] Fill human serum albumin solution with 4-arm-PEG-SG and FD&C blue #2 visualization reagent mixture solution into two-component syringes, spray and cross-link to form albumin-sealing gel. The gelation time after mixing was 2 seconds, the swelling degree of the prepared serum albumin gel was 138...
Embodiment 3
[0060] Weigh 2.5 g (25%, w / v) of plasma-derived human serum albumin and dissolve it in 10 ml of borate buffer (10 mM, pH 9.0) prepared with deionized water. Stir intermittently at 37°C for 1 hour to dissolve the protein. After the protein is completely dissolved and becomes a transparent liquid, use a vacuum pump for 30 minutes to quickly eliminate the bubbles in the solution.
[0061] Weigh 2 g of 4-arm polyethylene glycol ether tetrasuccinimide sebacate (4-arm-PEG-SSeb, molecular weight 12000D) and 15 mg of methylene blue visualization reagent, dissolve in 10 ml of phosphate buffer prepared by deionized water solution (10mM, pH=6.0), vortex to dissolve it into a transparent mixture liquid.
[0062] Plasma-derived human serum albumin solution, 4-arm-PEG-SSeb and methylene blue visualization reagent mixture solution were respectively filled into two-component syringes, sprayed and cross-linked to form serum albumin gel. The gelling time after mixing was 18 seconds, the swelli...
PUM
Property | Measurement | Unit |
---|---|---|
Bursting strength | aaaaa | aaaaa |
Bursting strength | aaaaa | aaaaa |
Bursting strength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com