Biogas residue decomposing agent with biogas output synergistic effect and application thereof
A technology of synergistic effect and decomposer, applied in microorganism-based methods, immobilized on/in organic carriers, biochemical equipment and methods, etc. Fermentation efficiency and service life and other issues, to achieve the effect of complete decomposition, shorten the fermentation cycle, and improve the utilization rate
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[0026] In the present invention, the preparation method of the biogas residue decomposing agent with the synergistic effect of biogas production preferably includes the following steps:
[0027] 1) Mix cellulase and xylanase to obtain a compound enzyme;
[0028] 2) Mixing the compound enzyme in step 1) with Bacillus subtilis and Bacillus licheniformis to obtain an enzyme-bacterial complex;
[0029] 3) Mixing the enzyme-bacteria complex in the step 2) with the carrier to obtain a biogas residue decomposing agent with a synergistic effect on biogas production.
[0030] In the present invention, the mixing method is not particularly limited, and a mixing scheme well known to those skilled in the art may be used.
[0031] The invention provides the application of the biogas residue decomposer with the synergistic effect of biogas production in improving biogas production and / or biogas residue degradation efficiency in biogas fermentation.
[0032] In the present invention, the raw material f...
Example Embodiment
[0035] Example 1
[0036] The original powder of Bacillus licheniformis and Bacillus subtilis, β-1,4-glucan-4-glucanohydrolase and β-1,4-endo-wood will be purchased from Shandong Gaolong Biotechnology Co., Ltd. Glycanase and β-xylosidase are mixed, and then defatted rice bran is added and mixed thoroughly so that the content of each component is 2.0 glucan-4-glucan hydrolase activity per gram of defatted rice bran ×10 5 U, β-1,4-endoxylanase and β-xylosidase are 1.0×10 5 U, the number of viable bacteria of Bacillus subtilis is 20 billion CFU, and the number of viable bacteria of Bacillus licheniformis is 10 billion CFU, and a biogas residue decomposing agent with a synergistic effect on biogas production is obtained.
Example Embodiment
[0037] Example 2
[0038] Mix the raw powder of Bacillus licheniformis and Bacillus subtilis purchased from Shandong Gaolong Biotechnology Co., Ltd. with β-1,4-glucan-4-glucanohydrolase and α-L-arabinosidase, Then add defatted rice bran and mix thoroughly so that the content of each component is the β-1,4-glucan-4-glucanohydrolase activity of 1.8×10 per gram of defatted rice bran 5 U, α-L-arabinosidase activity is 0.9×10 5 U, the number of viable bacteria of Bacillus subtilis is 18 billion CFU, and the number of viable bacteria of Bacillus licheniformis is 9 billion CFU, and a biogas residue decomposing agent with a synergistic effect on biogas production is obtained.
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